Serum bile acids profiling by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and its application on pediatric liver and intestinal diseases

Fu, Xiaowei; Xiao, Yingying; Golden, Jamie; Niu, Sizhe; Gayer, Christopher P.

doi:10.1515/cclm-2019-0354

Cited by 19 publications

(14 citation statements)

References 39 publications

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“…The majority of HPLC-MS/MS methods aimed at the analysis of major five BAs (i.e., CA, CDCA, DCA, LCA, UDCA) and their glycine and taurine conjugates with analysis times under 20 min and limits of detection in the nanomolar range [30,35,47,67,80]. Scherer et al [67] developed a rapid method (runtime of 6.5 min) for quantification of 18 BAs (free and conjugated).…”

Section: Hplc-msmentioning

confidence: 99%

Analysis of bile acids in human biological samples by microcolumn separation techniques: A review

2020

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Bile acids are a group of compounds essential for lipid digestion and absorption with a steroid skeleton and a carboxylate side chain usually conjugated to glycine or taurine. Bile acids are regulatory molecules for a number of metabolic processes and can be used as biomarkers of various disorders. Since the middle of the twentieth century, the detection of bile acids has evolved from simple qualitative analysis to accurate quantification in complicated mixtures. Advanced methods are required to characterize and quantify individual bile acids in these mixtures. This article overviews the literature from the last two decades (2000-2020) and focuses on bile acid analysis in various human biological samples. The methods for sample preparation, including the sample treatment of conventional (blood plasma, blood serum, and urine) and unconventional samples (bile, saliva, duodenal/gastric juice, feces, etc.) are shortly discussed. Eventually, the focus is on novel analytical approaches and methods for each particular biological sample, providing an overview of the microcolumn separation techniques, such as high-performance liquid chromatography, gas chromatography, and capillary electrophoresis, used in their analysis. This is followed by a discussion on selected clinical applications.

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Section: Hplc-msmentioning

confidence: 99%

Analysis of bile acids in human biological samples by microcolumn separation techniques: A review

2020

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“…Due to the wide-ranging and important functions of BAs, BA pro le analysis is garnering increasing attention in medicine and zootechnics (Fu et al 2020;Lin et al 2020). However, neither the reason for nor the effect of changes in BA pro les has received su cient attention in sh.…”

Section: Discussionmentioning

confidence: 99%

“…Even a slight difference in BA hydroxylation patterns may exert drastically different biological responses (Li and Chiang 2009;Song et al, 2015). Therefore, BA pro le assessments are garnering increasing interest to screen potential biomarkers for the early diagnosis of human liver and intestinal diseases (Fu et al 2020;Slopianka et al 2017), malnutrition (Lin et al 2020), and healing processes (Roda et al 2019). It was noticed that the BA pro les of sh could be in uenced by diet (Zhang et al 2017), however, it was unclear about the agent in diet that exerted the effect.…”

Section: Introductionmentioning

confidence: 99%

Dietary pectin caused greatly changes in bile acid profiles of Pelteobagrus fulvidraco

Cao¹,

Ren²,

Ni³

et al. 2021

Preprint

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To reveal the impact of dietary fiber (DF) on the bile acid (BA) profiles of fish, yellow catfish (Pelteobagrus fulvidraco) were fed diet containing 300 g kg-1 dextrin (CON diet, control) or pectin (a type of soluble DF, PEC diet) for 7 days, and then the BA profiles were analyzed by UHPLC-MS/MS. A total of 26 individuals of BAs were detected in the fish body, with 8, 10, 14, and 22 individuals of BAs were detected in the liver, serum, bile and hindgut digesta, respectively. The conjugated BAs (CBAs) of fish were dominated by taurine-CBAs (TCBAs). The concentrations of free BAs (FBAs) and the values of FBAs/CBAs in the bile of fish fed the PEC diet were nearly 5 and 7 times higher, respectively, than those in fish fed the CON diet. The values of glycine-CBAs/TCBAs in the liver, serum and bile of fish fed the PEC diet were significantly lower, and in the hindgut digesta was higher than that of fish fed the CON diet (P < 0.05). These results suggested that dietary pectin greatly changed the BA profiles of Pelteobagrus fulvidraco, attributed to inhibition of reabsorption of BAs. Therefore, attention should be paid to the impact to BA homeostasis when replacing fishmeal with DF-rich plant ingredients in the fish diet.

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“…The most abundant fragment ions were chosen for quantification purpose. Due to the lack of specific fragment ions, quantification of some unconjugated BA (UDCA, HDCA, CDCA, DCA) was accomplished by monitoring the same m/z values for both the precursor ion and the fragment ion, consistent with published literature [19]. LC conditions were optimized to achieve an optimal separation of three isobar groups of BA ( Figure 2).…”

Section: In Vivo Studymentioning

confidence: 99%

Bile Acids Quantification by Liquid Chromatography–Tandem Mass Spectrometry: Method Validation, Reference Range, and Interference Study

et al. 2020

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Bile acids (BA) play a pivotal role in cholesterol metabolism. Their blood concentration has also been proposed as new prognostic and diagnostic indicator of hepatobiliary, intestinal, and cardiovascular disease. Liquid chromatography tandem mass spectrometry (LC–MS/MS) currently represents the gold standard for analysis of BA profile in biological samples. We report here development and validation of a LC–MS/MS technique for simultaneously quantifying 15 BA species in serum samples. We also established a reference range for adult healthy subjects (n = 130) and performed a preliminary evaluation of in vitro and in vivo interference. The method displayed good linearity, with high regression coefficients (>0.99) over a range of 5 ng/mL (lower limit of quantification, LLOQ) and 5000 ng/mL for all analytes tested. The accuracies were between 85–115%. Both intra- and inter-assay imprecision was <10%. The recoveries ranged between 92–110%. Each of the tested BA species (assessed on three concentrations) were stable for 15 days at room temperature, 4 °C, and −20 °C. The in vitro study did not reveal any interference from triglycerides, bilirubin, or cell-free hemoglobin. The in vivo interference study showed that pools obtained from hyper-cholesterolemic patients and hyper-bilirubinemic patients due to post-hepatic jaundice for benign cholestasis, cholangiocarcinoma and pancreatic head tumors had clearly distinct patterns of BA concentrations compared with a pool obtained from samples of healthy subjects. In conclusion, this study proposes a new suitable candidate method for identification and quantitation of BA in biological samples and provides new insight into a number of variables that should be taken into account when investigating pathophysiological changes of BA in human diseases.

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Serum bile acids profiling by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and its application on pediatric liver and intestinal diseases

Cited by 19 publications

References 39 publications

Analysis of bile acids in human biological samples by microcolumn separation techniques: A review

Analysis of bile acids in human biological samples by microcolumn separation techniques: A review

Dietary pectin caused greatly changes in bile acid profiles of Pelteobagrus fulvidraco

Bile Acids Quantification by Liquid Chromatography–Tandem Mass Spectrometry: Method Validation, Reference Range, and Interference Study

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