Serum bile acids in liver cirrhosis promote neutrophil dysfunction

Balazs, I; Horvath, Angela; Leber, Bettina; Feldbacher, Nicole; Sattler, Wolfgang; Rainer, Florian; Fauler, Günter; Vermeren, Sonja; Stadlbauer, Vanessa

doi:10.1002/ctm2.735

Cited by 10 publications

(17 citation statements)

References 10 publications

(16 reference statements)

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“…ROS production was measured essentially as previously described 21 . Human neutrophils from healthy volunteers ( n = 3) were isolated with Percoll and resuspended in PBS++ (Dulbecco's PBS supplemented with Ca 2+ and Mg 2+ , 1 g/L D‐glucose and 4‐mM sodium bicarbonate) to a concentration of 6.25 × 10 6 cells/ml.…”

Section: Methodsmentioning

confidence: 99%

“…20 2.9 | Neutrophil function in vitro ROS production was measured essentially as previously described. 21 Human neutrophils from healthy volunteers (n = 3) were isolated with Percoll and resuspended in PBS++ (Dulbecco's PBS supplemented with Ca 2+ and Mg 2+ , 1 g/L D-glucose and 4-mM sodium bicarbonate) to a concentration of 6.25 Â 10 6 cells/ml. ROS production was measured indirectly using chemiluminescence in 5 Â 10 5 freshly isolated neutrophils per well at 37 C in luminescence-grade 96 well plates (Nunc, Thermo Fisher Scientific) in a Lumistar Omega luminescence microplate reader (BMG Labtech, Offenburg, Germany).…”

Section: Neutrophil Function Ex Vivomentioning

confidence: 99%

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Effect of albumin infusion on oxidative albumin modification and albumin binding capacity in chronic liver failure

Stauber,

Paar,

Balazs

et al. 2023

Basic Clin Pharma Tox

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Oxidative albumin modification and impaired albumin binding function have been described both in chronic liver failure and for therapeutic albumin solutions. The aim of the present study was to evaluate the effect of albumin infusion on redox state and binding function of circulating albumin. We studied 20 patients with cirrhosis who routinely received albumin infusions for prevention of post‐paracentesis circulatory dysfunction or treatment of hepatorenal syndrome. We measured albumin fractions by redox state of cysteine‐34 and albumin binding properties using dansylsarcosine as site II ligand. Therapeutic albumin solutions showed high contents of human nonmercaptalbumin‐1 and human nonmercaptalbumin‐2, exceeding the respective values in our patients with decompensated cirrhosis. An initial protocol for the first nine patients sampled at baseline, 24 h and 48 h after albumin infusion revealed no significant changes of oxidized albumin species or albumin binding properties. However, a modified protocol for the remaining 11 patients sampled at baseline, <1 h after and 24 h after albumin infusion revealed short‐lived changes of oxidized albumin species while no changes in albumin binding properties were observed. In conclusion, therapeutic albumin infusion transiently changed albumin redox state but did not improve binding function of circulating albumin in chronic liver failure.

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Section: Methodsmentioning

confidence: 99%

Section: Neutrophil Function Ex Vivomentioning

confidence: 99%

Effect of albumin infusion on oxidative albumin modification and albumin binding capacity in chronic liver failure

Stauber,

Paar,

Balazs

et al. 2023

Basic Clin Pharma Tox

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“… 34 Neutrophil phagocytic capacity (number of internalised bacteria per cell) of E. coli measured in whole blood or isolated neutrophils of patients with cirrhosis is impaired 51 , 52 , 57 , 59 , 60 or unchanged. 61 , 62 The percentage of phagocytic neutrophils is decreased in the blood of patients with cirrhosis 53 , 55 , 60 , 62 , 63 as well as in those with ACLF; 63 the degree of this dysfunction increases with increasing severity of cirrhosis and is higher in active drinkers compared to abstinent patients, but is not different between the aetiologies of cirrhosis (alcohol, HCV, autoimmune and other). 53 No defect in the phagocytosis of C. albicans was observed in patients with cirrhosis.…”

Section: Features Of Neutrophil Dysfunction In Cirrhosismentioning

confidence: 98%

“…Impaired neutrophil ROS production [51] , [52] , [53] , [54] , [55] , 58 , 59 , [65] , [66] , [67] , [68] and degranulation 50 , 64 have been described in cirrhosis and might contribute to impaired bacterial killing. 50 The alteration of ROS production in cirrhosis is characterised by an elevated percentage of neutrophils with basal ROS production (so-called “resting burst”) 51 , 55 , 58 , 60 , 62 , 65 and elevated, 59 , 65 unchanged 61 , 62 or reduced 63 , 67 intracellular basal ROS production (intracellular ROS levels). An increased percentage of neutrophils with basal ROS production has also been reported in ACLF.…”

Section: Features Of Neutrophil Dysfunction In Cirrhosismentioning

confidence: 99%

Circulating neutrophil anti-pathogen dysfunction in cirrhosis

Balazs,

Stadlbauer

2023

JHEP Reports

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“…Decreased concentrations of intestinal secondary bile acids following gut dysbiosis are shown in cirrhosis [124,125]. Studies have shown that the concentrations of circulatory primary bile acids produced by hepatocytes increased [100,105,[126][127][128], while the levels of intestinal secondary bile acids produced by the gut microbiome reduced in cirrhosis [124,125]. Therefore, a bile acids imbalance may affect the BBB with two bile acid-dependent approaches in cirrhosis:…”

Section: Altered Gut Microbial Metabolites and Molecules May Disrupt ...mentioning

confidence: 99%

Gut Dysbiosis and Blood-Brain Barrier Alteration in Hepatic Encephalopathy: From Gut to Brain

et al. 2023

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A common neuropsychiatric complication of advanced liver disease, hepatic encephalopathy (HE), impacts the quality of life and length of hospital stays. There is new evidence that gut microbiota plays a significant role in brain development and cerebral homeostasis. Microbiota metabolites are providing a new avenue of therapeutic options for several neurological-related disorders. For instance, the gut microbiota composition and blood-brain barrier (BBB) integrity are altered in HE in a variety of clinical and experimental studies. Furthermore, probiotics, prebiotics, antibiotics, and fecal microbiota transplantation have been shown to positively affect BBB integrity in disease models that are potentially extendable to HE by targeting gut microbiota. However, the mechanisms that underlie microbiota dysbiosis and its effects on the BBB are still unclear in HE. To this end, the aim of this review was to summarize the clinical and experimental evidence of gut dysbiosis and BBB disruption in HE and a possible mechanism.

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Serum bile acids in liver cirrhosis promote neutrophil dysfunction

Cited by 10 publications

References 10 publications

Effect of albumin infusion on oxidative albumin modification and albumin binding capacity in chronic liver failure

Effect of albumin infusion on oxidative albumin modification and albumin binding capacity in chronic liver failure

Circulating neutrophil anti-pathogen dysfunction in cirrhosis

Gut Dysbiosis and Blood-Brain Barrier Alteration in Hepatic Encephalopathy: From Gut to Brain

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