Serum antibody titers to <i>Bacteroides forsythus</i> in elderly subjects with gingivitis or periodontitis

Persson, G. Rutger; Schlegel-Bregenzer, Bettina; Chung, Whasun O.; Houston, L. L.; Oswald, Teresa K.; Roberts, Marilyn C.

doi:10.1034/j.1600-051x.2000.027011839.x

Cited by 11 publications

(11 citation statements)

References 21 publications

(30 reference statements)

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“…Even though this finding does not demonstrate the role of the S-layer as a virulence factor, it verifies that the S-layer interacts with the host to elicit a humoral immune response. Despite recent data confirming that B. forsythus is a predominant microorganism isolated from periodontal lesions (Chandad et al, 1997;Lai et al, 1987;Meurman et al, 1997;Moncla et al, 1991), it has been observed that not all B. forsythus strains induce strong immune responses in patients (Califano et al, 1997;Persson et al, 2000). The decreased immune reactivity to B. forsythus observed in some patients may not be attributed to the weak immunogenicity of B. forsythus, since B. forsythus was able to mount significant immune responses in rabbit and mice (Bird et al, 2001).…”

Section: Discussionmentioning

confidence: 87%

The surface (S-) layer is a virulence factor of Bacteroides forsythus

et al. 2003

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Bacteroides forsythus has emerged as a crucial periodontal pathogen with possible implications for systemic disease. The aim of this study was to isolate the S-layer from B. forsythus and examine its virulence potential as a part of efforts to characterize virulence factors of B. forsythus. The role of the S-layer in the haemagglutinating and adherent/invasive activities was evaluated. It was observed that the S-layer alone was able to mediate haemagglutination. In adherent and invasive studies, transmission electron microscopy clearly revealed that B. forsythus cells were able to attach to and invade KB cells, showing the formation of a microvillus-like extension around adherent and intracellular bacteria. The quantitative analysis showed that five different B. forsythus strains exhibited attachment (1?9-2?3 %) and invasion (0?4-0?7 %) capabilities. It was also observed through antibody inhibition assays that adherent/invasive activities of B. forsythus are mediated by the S-layer. Furthermore, an in vivo immunization study adopting a murine abscess model was used to prove that the S-layer is involved in the infectious process of abscess formation. While mice immunized with purified S-layer and B. forsythus whole cells did not develop any abscesses when challenged with viable B. forsythus cells, unimmunized mice developed abscesses. Collectively, the data obtained from these studies indicate that the S-layer of B. forsythus is a virulence factor.

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Section: Discussionmentioning

confidence: 87%

The surface (S-) layer is a virulence factor of Bacteroides forsythus

et al. 2003

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“…50 High antibody titers to T forsythensis have also been reported to be significantly higher in periodontitis patients. 51 Finally, although the role of antibodies to periodontal organisms in host immune protection against infections by periodontal organisms is not fully elucidated, it appears that antibody level is not a good quantitative indicator for periodontal disease. Nonetheless, antibody to periodontopathic organisms appears to be a reliable index measure for systemic exposure to these organisms.…”

Section: Discussionmentioning

confidence: 99%

Periodontal Disease and Coronary Heart Disease

et al. 2005

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Background-Results from studies relating periodontal disease to cardiovascular disease have been mixed. Residual confounding by smoking and use of clinical measures of periodontal disease rather than measures of infection have been 2 major criticisms. The aims of this study were to investigate relationships between prevalent coronary heart disease (CHD) and 2 exposures, (1) clinical periodontal disease and (2) IgG antibodies to 17 oral organisms, and to evaluate the role of smoking in these relationships. Methods and Results-Our study is based on a subset of participants in the Atherosclerosis Risk in Communities (ARIC)Study, who received a complete periodontal examination during visit 4 (1996 -1998). The exposures were periodontal status and serum IgG antibody levels against 17 periodontal organisms, and the outcome was prevalent CHD at visit 4. Multivariable analyses indicate that periodontal status is not significantly associated with CHD in either ever smokers or never smokers. Similar analyses evaluating antibodies indicate that high antibodies (above the median) to Treponema denticola (odds ratio [OR]ϭ1.7; 95% CI, 1.2 to 2.3), Prevotella intermedia (ORϭ1.5; 95% CI, 1.1 to 2.0), Capnocytophaga ochracea (ORϭ1.5; 95% CI, 1.1 to 2.1), and Veillonella parvula (ORϭ1.7; 95% CI, 1.2 to 2.3) are significantly associated with CHD among ever smokers, whereas Prevotella nigrescens (ORϭ1.7; 95% CI, 1.1 to 2.6), Actinobacillus actinomycetemcomitans (ORϭ1.7; 95% CI, 1.2 to 2.7), and Capnocytophaga ochracea (ORϭ2.0; 95% CI, 1.3 to 3.0) were associated with CHD among never smokers. Conclusions-Clinical signs of periodontal disease were not associated with CHD, whereas systemic antibody response was associated with CHD in ever smokers and never smokers. These findings indicate that the quality and quantity of the host response to oral bacteria may be an exposure more relevant to systemic atherothrombotic coronary events than clinical measures.

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“…the 44 and 27 kDa proteins) (Sims et al 1999). Studies of clinical isolates of T. forsythia have also demonstrated a large and subject‐defined genotypic variation for T. forsythia (Persson et al 2000). P. gingivalis FDC 381 possesses a 53 kDa protein antigen (Ag53) on its outer membrane, which evokes a strong humoral immune response in many patients with periodontal disease, but the humoral immune responses to Ag53 differ greatly among patients and are a result of major B‐cell epitopes (Oyaizu et al 2001).…”

Section: Vaccine Candidate Antigens Of P Gingivalismentioning

confidence: 99%