Serum antibody responses of Texel sheep experimentally infected with Haemonchus contortus

Schallig, Henk D. F. H.; Leeuwen, M. A. W. Van; Bernadina, W.E.; Hendrikx, W.M.L.

doi:10.1016/0034-5288(94)90083-3

Cited by 23 publications

(12 citation statements)

References 28 publications

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“…Schalling et al (1994) observed in Texel sheep that there is an induction of the antibody response by challenge, producing a more rapid and lasting response, which suggests an immune memory. The increase in serum antibodies observed in the present and previous studies does not necessarily indicate that they are responsible for resistance, since the location of H. contortus larvae and adults is only in the abomasum.…”

Section: Discussionmentioning

confidence: 99%

“…Most studies performed to assess the response to haemonchosis have used single infections with a large number of H. contortus larvae (Schalling et al 1994;Gómez-Muñoz et al 1999;Terefe et al 2005) or natural infections (Bricarello et al 2003). The natural ingestion of larvae is performed gradually; thus, results generated from massive single infections probably do not reflect the pattern of a natural infection (Mugambi et al 1996).…”

Section: Discussionmentioning

confidence: 99%

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Correlation of parasitological and immunological parameters in sheep with high and low resistance to haemonchosis

Muñoz-Guzmán¹,

Cuéllar-Ordaz²,

Valdivia-Anda³

et al. 2006

Can. J. Anim. Sci.

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Correlation of parasitological immunological parameters in sheep with high and low resistance to haemonchosis. Can. J. Anim. Sci. 86: 363-371. Parasitological and immunological parameters of Blackbelly (Bb), a haemonchosis high-resistance sheep breed, and Columbia (Cb) a haemonchosis low-resistance breed were compared. Two experiments were performed: Bb and Cb lambs were infected weekly with 1000 third-stage larvae (L3) of Haemonchus contortus for 6 wk. In the first experiment, faecal egg counts (FEC) and blood eosinophils (BE) were determined for 15 wk. In the second experiment, the following parameters were measured weekly: FEC, plasma proteins, serum IgG to H. contortus L3 levels by ELISA and L3 antigens recognized by Western blot; animals were euthanized after 15 weeks and autopsies were performed to evaluate the total adult phases (AP), eosinophils and mast cells in the fundic abomasal region (FAR), pyloric abomasal region (PAR) and abomasal lymph node (ALN).Fecal egg counts and AP were significantly higher (P < 0.05) in Cb lambs. BE showed a significant increase (P < 0.05) in both breeds at week 9 and, subsequently, Cb lambs presented a sudden decrease in these levels. The Bb lambs revealed a higher amount (P < 0.05) of antibodies between weeks 9 and 15 of the experiment, and Cb lambs showed a reduction in antibody levels at week 9. The Western blot revealed 11 bands of H. contortus L3 antigens; no differences were observed between breeds. The number of tissue eosinophils per cm 2 in PAR and ALN was higher (P < 0.05) in the Bb lambs. No differences were found (P > 0.05) in the number of mast cells per cm 2 in the sampled regions of both infected groups. The increase and maintenance of serum antibodies and circulating and local eosinophils in resistant animals agrees with the development and maintenance of a Th 2 type immune response. The initial increase and further reduction of serum antibodies and circulating and local eosinophils in the low-resistance lamb breed suggest an initial Th 2 type response and a subsequent change to a Th 1 type response. Les animaux ont été sacrifiés au bout de 15 semaines et on a pratiqué une autopsie pour évaluer le nombre total de phases adultes du parasite (PA), d'éosinophiles et de mastocytes dans la région gastrique (RG), la région pylorique (RPC) et les ganglions lymphatiques (GL) de la caillette. Le nombre d'oeufs et de PA dans les fèces était significativement plus élevé (P < 0,05) chez les agneaux Cb. Les auteurs ont relevé une hausse significative (P < 0,05) du NES chez les deux races la neuvième semaine; par la suite, cette concentration baissait subitement chez les agneaux Cb. Les agneaux Bb présentaient une concentration plus élevée (P < 0,05) d'anticorps entre la 9 e et la 15 e semaine de l'expérience alors que cette concentration était plus basse chez les agneaux Cb, la neuvième semaine. Le transfert de Western révèle 11 bandes pour les antigènes des L3 de H. contortus; aucune variation n'a été relevée à cet égard entre les deux races. Les agneaux Bb présentaie...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Correlation of parasitological and immunological parameters in sheep with high and low resistance to haemonchosis

Muñoz-Guzmán¹,

Cuéllar-Ordaz²,

Valdivia-Anda³

et al. 2006

Can. J. Anim. Sci.

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“…The purification process was monitored by standard SDS-PAGE on a 12 . 5% gel under non-reducing conditions and subsequent Western blotting using pooled serum from sheep hyperimmunized by repeated infections with H. contortus (Schallig et al 1994b). Protein concentrations were determined using the bicinchoninic assay (Pierce, Rockford, USA).…”

Section: Parasite and Preparation Of Antigenmentioning

confidence: 99%

“…2 mg.kg ¹1 ) and subsequently challenged at week 10 as described above. This group was used to assess the degree of immunity developing as a result of H. contortus infection (Schallig et al 1994b(Schallig et al , 1995. Group 4 (n ¼ 5) was used as an adjuvant control group and was injected three times with 2 ml dimethyl dioctadecyl ammonium bromide (DDA, 2 .…”

Section: Immunization Trial and Parasitologymentioning

confidence: 99%

Protective immunity induced by vaccination with two Haemonchus contortus excretory secretory proteins in sheep

Schallig

Leeuwen

Cornelissen

1997

Parasite Immunology

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Two excretory secretory (ES) antigens of adult Haemonchus contortus with molecular weights of 15 and 24 kDa, respectively, were evaluated as protective immunogen against haemonchosis. Sheep were vaccinated three times and subsequently challenged with 20,000 infective larvae. Vaccination induced significant reduction (> 70%) in mean faecal egg counts and abomasal worm burden compared to the non-vaccinated control group or adjuvant control group. Vaccination induced ES-specific antibodies and stimulated infiltration of mast cells in the abomasal tissue.

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“…Treated and non-treated blots were incubated with pooled sera from non-infected sheep that were raised under worm-free conditions or with pooled sera from sheep hyperimmunised for H. contortus (Schallig et al 1994a). The test sera were diluted 1:100 in PBS containing 5% (w/v) skimmed milk.…”

Section: Probing Blots With Seramentioning

confidence: 99%

Carbohydrate epitopes on Haemonchus contortus antigens

Schallig

Leeuwen

1996

Parasitol Res

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Extracts of infective larvae and adults of the trichostrongylid Haemonchus contortus were studied for the presence of carbohydrate moieties. Several different lectin-binding sites were demonstrated in both stages using a panel of nine lectins. The carbohydrate specificity of the lectins used strongly suggests that alpha-D-mannose, alpha-D-glucose, and D-N-acetylglucosamine are the most important carbohydrate epitopes present on H. contortus proteins. Thus, N-linked oligosaccharides form the major part of the carbohydrate moieties on these glycoproteins. Treatment with sodium periodate was performed to investigate the immunoreactivity towards the carbohydrate moieties. This treatment resulted in a reduction in the immunoreactivity of these antigens as demonstrated by enzyme-linked immunosorbent assay (ELISA) and immunoblotting, suggesting that a substantial part of the host immune response against H. contortus is directed against the carbohydrate epitopes on the parasite antigens.

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Serum antibody responses of Texel sheep experimentally infected with Haemonchus contortus

Cited by 23 publications

References 28 publications

Correlation of parasitological and immunological parameters in sheep with high and low resistance to haemonchosis

Correlation of parasitological and immunological parameters in sheep with high and low resistance to haemonchosis

Protective immunity induced by vaccination with two Haemonchus contortus excretory secretory proteins in sheep

Carbohydrate epitopes on Haemonchus contortus antigens

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