Serum antibody response of Castellana sheep to Haemonchus contortus infection and challenge:

Gómez-Muñoz, María Teresa; Cuquerella, Montserrat; Gómez-Iglesias, L.A; Méndez, Susana; Fernández-Pérez, Francisco; Fuente, Concepción de la; Alunda, José María

doi:10.1016/s0304-4017(98)00260-x

Cited by 41 publications

(20 citation statements)

References 28 publications

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“…But in contrast to studies in Texel and Castellana sheep showing a stronger IgG and IgA response in animals previously exposed to infection (Schallig et al, 1995;Gomez-Munoz et al, 1999), in Creole kids naturally parasitized on pasture before the experimental infection, a weak response was observed except for IgA anti-ESP which showed amounts similar to primo-infected sheep. Previous studies in sheep suggest that IgA may be the major mechanism controlling fecundity of H. contortus and worm length and fecundity of Teladorsagia cicumcincta (Stear et al, 1995b;Strain et al, 2002 (Amarante et al, 2005).…”

Section: Discussioncontrasting

confidence: 94%

Serum antibody responses in Creole kids experimentally infected with Haemonchus contortus

Bambou¹,

Chevrotière²,

Varo³

et al. 2008

Veterinary Parasitology

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The objective of this study was to evaluate the relationship of parasite-specific serum antibodies with the resistance status of Creole kids. The average breeding values on egg output predicted in a context of natural infection at 11 months of age were distant of 1.07 genetic standard deviation between resistant and susceptible animals. After drenching the animals were maintained worm-free during 1 month until experimental infection with 10,000 Haemonchus contortus infective larvae (L3). Enzyme-linked immunosorbent assay was carried out in serum samples to determine the level of IgG, IgA and IgE anti-H. contortus L3 crude extracts and adult excretion/secretion products (ESP). Parasitological and blood immunological parameters were measured on the 2 extreme groups. Despite the absence of any typical signs of haemonchosis, susceptible kids had more than 11 times higher faecal egg counts (FEC) at 35 days post-infection (d.p.i.) than resistant kids had. Levels of immunoglobulin against H. contortus L3 and ESP increased significantly after infection in both groups. However, no difference in the host immune response mediated by immunoglobulin against H. contortus was evidenced between groups. This finding suggests that, in goats previously infected by H. contortus, a degree of protection occurred and the phenotypic and genetic segregation in resistant and susceptible animals were not related to the humoral immune response. The correlation coefficients between FEC and IgE anti-ESP (r = 0.593; P < 0.05 was significant in both resistant and susceptible animals. Such correlation suggesting a hypersensitivity reaction dependent on worm prolificacy has never been described. This result needs further studies to understand the mechanisms underlying this observation. #

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Section: Discussioncontrasting

confidence: 94%

Serum antibody responses in Creole kids experimentally infected with Haemonchus contortus

Bambou¹,

Chevrotière²,

Varo³

et al. 2008

Veterinary Parasitology

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“…Eosinophil, mast cell, and globule leukocyte recruitments were apparent at 7, 15 and 28 dpc respectively in primaryinfected animals while these cells were still present in previously-infected lambs at 3 dpc. Antibody responses were delayed and low in primary-infected lambs and rapid and higher in previously-infected ones as previously shown by Schallig et al [30] and Gomez-Munoz et al [16]. Consequently, while final response intensities were similar in both infected groups, recruitment of cells and secretion of immunoglobulins in previously-infected sheep were observed before their occurrence in primary-infected sheep.…”

Section: Discussionsupporting

confidence: 81%

Haemonchus contortus(Nematoda: Trichostrongylidae) infection in lambs elicits an unequivocal Th2 immune response

Nguyen²,

et al. 2006

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-Selection of resistant animals and host immunization have been proposed as alternative methods for the control of gastrointestinal nematode parasites. However, a better knowledge of the mechanisms involved in protective immunity against these parasites is required for the development of optimal strategies. In this study, 3 month old INRA 401 lambs (n = 81) were allocated into three groups: uninfected control, challenged either once (primary-infected animals) or twice (previouslyinfected animals) with 10 000 Haemonchus contortus L 3 . Uninfected control and challenged animals were sequentially sacrificed at 3, 7, 15 and 28 days post challenge. In both challenged groups, a clear Th 2 -oriented immune response was observed in the abomasal lymph node and mucosa. IL-4 and IL-13 mRNA over-expression, recruitment of eosinophils, mast cells and globule leukocytes and production of specific systemic IgG and mucosal IgA were observed earlier in previously-infected animals than in primary-infected ones. At 28 days post infection, no differences between intensities of these responses were observed between the challenged groups. Worm establishment rates were similar in previously-infected and primary-infected lambs. However, reductions of worm development, female fecundity and fecal egg output were observed in previously-infected sheep. We conclude that H. contortus infection in young INRA 401 lambs induced an unequivocal Th 2 immune response resulting in the regulation of worm egg production without affecting their establishment.Haemonchus contortus / sheep / Th 2 immune response / quantitative PCR / antibody and cellular response

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“…The finding of this experiment is in contrast to previous studies in sheep that there is normally an increase in peripheral antibodies in animals previously exposed to GIN infection (Schallig et al, 1995;Gómez-Muñoz et al, 1999). The discrepancy is likely due to goats and sheep having developed their own strategies to regulate GIN infection, based on immune mechanism and feeding behaviours .…”

Section: Discussioncontrasting

confidence: 56%

Effects of dietary protein supplementation and plane of nutrition on the resistance and the resilience of Boer goats against artificial Haemonchus contortus infection under confined conditions

Can¹

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The effects of protein and/ or energy supplementation have been well studied in sheep. However, the few pen studies conducted with goats have failed to identify the role of protein supplementation and combinations with energy supplements against H. contortus infection.The general objective of this thesis was to investigate the effects of protein supplementation and plane of nutrition on resistance and resilience of Boer goats to infection from Haemonchus contortus. To achieve this the first experiment was designed to determine the appropriate amount of additional dietary protein (by having three groups of mature goats effectively fed three different levels of dietary protein) required by nematode free animals given a single relatively high dose of infective H. contortus larvae, to not become infected or at least develop resistance and/or resilience. It was assumed, based on the scientific literature, that a single relatively high dose of infective larvae would elicit strong pathogenic effects where additional dietary protein would give some assistance to the does with their defense to Haemonchus. As often is the case with research on goats the results were not unequivocally clear.Based on these results it was clear that a single dose of infective larvae was insufficient to become established and that these animals had already developed some level of immune response and thus it was assumed, again based on the scientific literature, to elicit strong pathogenic effects the animals tested needed to be younger (more naïve to Haemonchus) and with greater i.e. continued exposure to infective larvae as would happen under grazing conditions (trickle infection) such that different quantities of dietary protein would give these animals different responses to infection by Haemonchus. Again the responses by the goats only indicated subtle differences in immune responses against trickle H. contortus L3 infection. These results, again unexpected, indicated that level of intake (or more specifically plane of nutrition, prior to or during exposure) may be at least as important as protein supplementation in reasonably well fed animals; and or that the method of infection (oral drenching with L3 larvae) was possibly an unreliable method of infection requiring a more consistent method of infection. As such the third experiment involved nematode free mature animals (the only animals available at that time) fed on very different planes of nutrition preceding and during the experiment given relatively high trickle doses of H. contortus larvae via intra-ruminal infection.Overlaid across these experiments were constraints imposed on the amount of infective L3 larvae used to achieve infection; 100 L3 larvae per kg live-weight as a single dose was the maximum initially approved by the University Animal Ethics committee, as they had concerns about animals dying, they also stipulated the use of oral drenching for infective larvae. It was only after these methods failed ii to elicit a strong response was approval given by the Univers...

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Serum antibody response of Castellana sheep to Haemonchus contortus infection and challenge:

Cited by 41 publications

References 28 publications

Serum antibody responses in Creole kids experimentally infected with Haemonchus contortus

Serum antibody responses in Creole kids experimentally infected with Haemonchus contortus

Haemonchus contortus(Nematoda: Trichostrongylidae) infection in lambs elicits an unequivocal Th2 immune response

Effects of dietary protein supplementation and plane of nutrition on the resistance and the resilience of Boer goats against artificial Haemonchus contortus infection under confined conditions

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