Serum Antibody Response Elicited by a Casein Diet is Directed to Only Limited Determinants of αs1-Casein

Abstract

Feeding mice with bovine whole casein as a diet constituent elicited an anti-αs1-casein (αs1-CN) systemic humoral response. The mice fed with the casein (CN) diet responded poorly to subsequent parenteral immunization with the antigen, indicating that oral tolerance was induced in the animals. The serum antibodies elicited by feeding CN were tested for binding to a panel of synthetic peptides and are shown to recognize only a limited number of potential antigenic determinants on αs1-CN. Our results suggest that a similar mechanism may be involved in the onset of milk allergy.

Oral administration of bovine whey proteins to mice elicits opposing immunoregulatory responses and is adjuvant dependent

“…The inability to suppress antibody responses with WP feeding, as reported here, appears to be in contrast with other studies using similar milk proteins [13][14][15][28][29][30]. Various mouse strains such as CH3/HeJ, BALB/c (H-2 d ) and C57BL/6 (H-2 b ) fed as 1-casein (a milk derived protein) were susceptible to tolerance induction though effects on antibody responses were variable [13]. One study described the suppression of antibody responses to as 1-casein after a high antigen dose feed.…”

“…Suppression of specific OVA antibodies (including IgG1 and IgG2a subclasses) and DTH responses has also been reported [1,4,8,25,27]. The inability to suppress antibody responses with WP feeding, as reported here, appears to be in contrast with other studies using similar milk proteins [13–15,28–30]. Various mouse strains such as CH3/HeJ, BALB/c (H‐2 d ) and C57BL/6 (H‐2 b ) fed α s 1‐ casein (a milk derived protein) were susceptible to tolerance induction though effects on antibody responses were variable [13].…”

“…Numerous studies, whilst using different milk proteins, such as whey and caseins, have shown suppression of IgG responses after feeding [13–15]. The doses fed, though in excess of 1 mg/g body weight, confirmed a possible difference in susceptibility to suppression of cellular and antibody responses during OT induction.…”

Ingestion of Casein Hydrolysate Induces Oral Tolerance and Suppresses Subsequent Epicutaneous Sensitization and Development of Anaphylaxis Reaction to Casein in Mice

“…Moreover, studies regarding the oral tolerance induced by casein and its hydrolysates demonstrated suppression of the sensitization to casein in mice [15-17]. The oral tolerance-inducing ability of casein hydrolysate was first reported in the 1990s [16].…”

Induction of Oral Tolerance in Mice by Continuous Feeding with β-Lactoglobulin and Milk

“…In the course of their studies on oral tolerance to milk casein, Kim et al and Hachimura et al have reported that the speciˆc antibody response elicited by the casein diet was directed towards only limited determinants. 21,22) We have shown here that not only some of the major epitopes escaped oral tolerance, but also the dose of the antigen strongly aŠected the epitope speciˆcity. It was also noted that the similar dose of BLG from 0.3 W BLG and milk feeding caused a diŠerent immune response in respect of the antibody speciˆcity to BLG.…”