SERUM ANTIBODY RESPONSE AND NASAL LYMPHOID TISSUE (NALT) STRUCTURE IN THE ABSENCE OF IL-4 OR IFN-γ

Andoh, A; Masuda, Arata; Kumazawa, Yoshio; Kasajima, Takeshi

doi:10.1006/cyto.2002.1980

Cited by 4 publications

(6 citation statements)

References 16 publications

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“…Sera antibody responses accompanying these immunization regimes were predominantly IgG (81, 82). Similar findings were obtained in gene knockout mice (92). IgA was not detectable in sera or gut washes but was found readily in nasal washes (81, 82).…”

Section: Immune Responsessupporting

confidence: 86%

“…In other experiments, IN antigen exposure elicited IgA responses in salivary, respiratory, intestinal, and vaginal secretions (86, 94). IN infection with influenza virus (92) or respiratory syncytial virus (93) elicited antigen‐specific IgA‐secreting cells in the NALT and cervical lymph nodes, again suggesting NALT cell traffic. These findings are consistent with NALT cell trafficking capability, especially to distant mucosae and to peripheral lymph nodes (82, 91), but this finding has yet to be confirmed by specific cell trafficking studies.…”

Section: Immune Responsesmentioning

confidence: 99%

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Bronchus‐ and nasal‐associated lymphoid tissues

Bienenstock

McDermott

2005

Immunological Reviews

175

142

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The bronchus-associated lymphoid tissue (BALT) and the nasal-associated lymphoid tissue (NALT) constitute organized lymphoid aggregates that are capable of T- and B-cell responses to inhaled antigens. BALT, located mostly at bifurcations of the bronchus in animals and humans, is present in the fetus and develops rapidly following birth, especially in the presence of antigens. Humoral immune responses elicited by BALT are primarily immunoglobulin A secretion both locally and by BALT-derived B cells that have trafficked to distant mucosal sites. Similarly located T-cell responses have been noted. On the basis of these findings, the BALT can be thought of as functionally analogous to mucosal lymphoid aggregates in the intestine and is deemed a member of the common mucosal immunologic system. NALT has been described principally in the rodent nasal passage as two separate lymphoid aggregates. It develops after birth, likely in response to antigen, and B- and T-cell responses parallel those that occur in BALT. It is not known whether NALT cells traffic to distant mucosal sites, although mucosal responses have been detected after nasal immunization. NALT appears from many studies to be a functionally distinct lymphoid aggregate when compared with BALT and Peyer's patches. It may exist, however, in humans as a diffuse collection of isolated lymphoid follicles.

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Section: Immune Responsessupporting

confidence: 86%

Section: Immune Responsesmentioning

confidence: 99%

Bronchus‐ and nasal‐associated lymphoid tissues

Bienenstock

McDermott

2005

Immunological Reviews

175

142

View full text Add to dashboard Cite

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“…Construction, amplification, purification of nonreplicative recombinant adenovirus expressing the human TSHR A subunit (Ad‐TSHR289) and determination of the viral particle concentration were performed as previously described [7]. Female wild type (wt) BALB/c mice (eight to 12 weeks old; Charles River Laboratory Inc., Tokyo, Japan) and IFN‐ γ KO and IL‐4 KO BALB/c mice (Jackson Laboratory Inc., Bar Harbor, ME, USA) [8] were injected in the quadriceps with 100 µ l PBS containing 10 10 particles of Ad‐TSHR289 twice at a three‐week‐interval. Blood, spleens and thyroid tissues were obtained two weeks after the second immunization.…”

Section: Methodsmentioning

confidence: 99%

TSH receptor-adenovirus-induced Graves’ hyperthyroidism is attenuated in both interferon-γand interleukin-4 knockout mice; implications for the Th1/Th2 paradigm

Nagayama

Saitoh

McLachlan

et al. 2004

Clinical and Experimental Immunology

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SUMMARYThe role of the Th1/Th2 balance in the pathogenesis of murine Graves' hyperthyroidism is controversial. In BALB/c mice injected with adenovirus expressing TSH receptor (TSHR-adeno model), we found that suppression of TSHR-specific Th1 immune responses by exogenous interleukin-4 (IL-4), agalactosylceramide or helminth ( Schistosoma mansoni ) infection was associated with inhibition of hyperthyroidism, indicating the critical role for Th1 cytokines. In contrast, BALB/c IL-4 knockout (KO), but not interferon-g (IFN-g ) KO mice failed to develop Graves' hyperthyroidism when injected with TSHR-expressing M12 B lymphoma cells (TSHR-M12 model), suggesting the importance of Th2 cytokine IL-4. To reconcile differences in these two models, we used IL-4 KO and IFN-g KO BALB/c mice in the TSHR-adeno model. Unlike wild-type (wt) BALB/c mice in which 60% developed hyperthyroidism, only 13 and 7% of IL-4 KO and IFN-g KO mice, respectively, became hyperthyroid. Thyroid stimulating antibodies were positive in most hyperthyroid mice. TSHR antibody titres determined by TSH binding inhibition and ELISA were comparable in all three groups. IgG1 and IgG2a TSHR antibody titres were similar in IFN-g KO and wt mice, whereas IgG1 TSHR antibody titres and TSHRspecific splenocyte IFN-g secretion were lower in IL-4 KO than in IFN-g KO and wt mice, respectively. Our results clearly implicate both IFN-g and IL-4 in development of hyperthyroidism in the TSHRadeno model. These data, together with the previous report, also indicate different cytokine requirements in these two Graves' models, with IFN-g being more important in the TSHR-adeno than the TSHR-M12 model. Moreover, our previous and present observations indicate a difference in the role of exogenous versus endogenous IL-4 in TSHR-adenovirus induced Graves' hyperthyroidism.

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“…Nesse ponto, as vacinas administradas pelas vias intranasal ou oral induzem a produção de IgA local via NALT e o desenvolvimento de imunidade mediada por células específicas nos tratos respiratório e gastrointestinal, respectivamente. A IgA local protege contra a invasão de agentes infecciosos e é um benefício significativo em doenças que envolvem principalmente estas áreas (DHEIN;GORHAM, 1986;STRAVER, 1989;HALL et al, 1991;HERITAGE et al, 1997HERITAGE et al, , 1998ANDOH et al, 2002;HISHIKI et al, 2004).…”

Section: Vias De Administraçãounclassified

“…Muitos estudos identificaram que existe grande contribuição local de IgA à defesa imune do trato respiratório contra uma variedade de agentes potencialmente patogênicos associada a papel específico de tecidos linfoides associados à mucosa, como o NALT (DHEIN;GORHAM, 1986;HERITAGE et al, 1997HERITAGE et al, , 1998ANDOH et al, 2002;HISHIKI et al, 2004).…”

Section: Pesquisa De Anticorposunclassified

<b>Influência etária na resposta imunológica de bezerros à vacinação intranasal</b>

Gomes¹

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Antes de todos, a Deus e ao meu anjo da guarda, tenho a certeza que estavam segurando minha mão a cada passo. À minha família, pelo apoio moral, amoroso e muitas vezes financeiro. Aos meus tios Humberto e Cleonice, não tenho palavras para demonstrar minha gratidão por terem me acolhido por todos esses anos com atenção e carinho. Aos meus primos Marta e Thiago, obrigada pelas conversas, paciência e convívio. Marta, muito obrigada pelo carinho, companhia e ajuda com os bezerros, e por me dar soluções para problemas no biotério. Thiago te agradeço pelas inúmeras vezes que me ajudou tecnologicamente sempre que tive problemas com os eletrônicos rss. A Eunice, por ter me ajudado tanto em casa sempre que precisei. À minha orientadora Alice, aqui agradeço mais uma vez a Deus por ter te colocado no meu caminho... ou devo agradecer por eu ter me colocado no seu ao me sentar ao seu lado no ônibus e pedindo para me orientar? Você é uma pessoa sensacional e que me recebeu de coração aberto sem nem mesmo me conhecer. Obrigada por me ajudar a crescer pessoal e intelectualmente. À equipe Della Libera, Heloisa, você é uma amiga de outras vidas e minha sub-orientadora. Obrigada por ser o que é e por me ajudar sempre que pedi. Camila, apesar de sermos totalmente diferentes, nossa amizade se ajustou e se aprofundou. Agradeço muito por ter dado certo irmos juntas ao Canadá, pois nos tornamos grandes parceiras. Obrigada pela constante prontidão em ajudar e pelo carinho. Bruna, foi seu otimismo que me fez acreditar que podia entrar na USP. Gabriela, obrigada pelos momentos felizes e descontraídos. Kamila, você também é uma grande parceira e está sempre à disposição para ajudar em tudo e a todos. Fernando, nosso gênio amigo. Te agradeço imensamente pelas inúmeras ajudas e por acender muitas lâmpadas na minha cabeça. Rodrigo. Obrigada pelas suas idéias criativas e pela sua positividade. Maiara, obrigada por nos ajudar tanto, de longe e de perto. Jéssyca, nossa caçula. Obrigada por nos socorrer nos momentos em que mais precisamos.

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SERUM ANTIBODY RESPONSE AND NASAL LYMPHOID TISSUE (NALT) STRUCTURE IN THE ABSENCE OF IL-4 OR IFN-γ

Cited by 4 publications

References 16 publications

Bronchus‐ and nasal‐associated lymphoid tissues

Bronchus‐ and nasal‐associated lymphoid tissues

TSH receptor-adenovirus-induced Graves’ hyperthyroidism is attenuated in both interferon-γand interleukin-4 knockout mice; implications for the Th1/Th2 paradigm

<b>Influência etária na resposta imunológica de bezerros à vacinação intranasal</b>

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