Serum antibodies to oral Bacteroides asaccharolyticus (Bacteroides gingivalis): relationship to age and periondontal disease

The influence of periodontal therapy on serum antibody titers to selected periodontal disease-associated microorganisms was assessed in 23 patients having chronic inflammatory periodontal disease (CIPD). The immunoglobulin G (IgG) titers were determined by the microELISA technique in serum samples obtained prior to treatment; following a hygienic phase which included scaling, root planing, and oral hygiene instruction; following surgical treatment; and one year and two years following hygienic phase (maintenance phase). Considerable individual variability existed in the magnitude of immune response to specific bacterial preparations. Significant reductions in the mean antibody titers were seen to A. viscosus, S. sanguis, F. nucleatum, S. sputigena, B. gingivalis, B. intermedius, B. melaninogenicus, T. vincentii, and T. denticola by the end of the second year of maintenance. There was no consistent response to Capnocytophaga. When individual patient responses were examined, 6 of the 23 were found to have elevated titers to at least one of the microorganisms in the interval between pretreatment and the end of the hygienic phase; however, in all but one case, the titers at the end of the second year of maintenance were below pretreatment levels. Antibody levels to bacteria such as S. sanguis were modified during therapy. This would indicate that immune responses to microbes not generally considered to be "periodontal pathogens" may be modified by adjuvant activity associated with subgingival plaque or changes in the environment of the sulcus and that subsequent changes in titer do not necessarily reflect a role of that microorganism in the disease process.

Section: Discussionmentioning

confidence: 99%

“…Hofstad 1984, Naito et al 1985, Tew et al 1985a. Other studies have shown that antibodies to many of these species appear normally in the sera during maturation from the neonate to the adolescent (Mouton et al 1981). Thus, ••preinfection" sera do not usually exist and "normal" antibody titers to these microorganisms vary considerably.…”

mentioning

confidence: 99%

The effects of periodontal therapy on serum antibody (IgG) levels to plaque microorganisms*

Aukhil

Lopatinct

Syed

et al. 1988

“…As discussed by Ebersole et al (1985), a major consideration of many studies is that the diseases are treated as static phenomena and do nol consider the apparent cyclical nature of disease progression described by Goodson et al (1982). Mouton et al (1981) described the increase in serum antibody reactive with B. gingivalis in adult periodontitis patients and in patients with the generalized form of juvenile periodontitis. Tew et al (1985) found that subjects with severe periodontitis were, most often, seropositive for Eubacterium brachy.…”

Section: Discussionmentioning

confidence: 99%

“…Most attempts to date have centered on elucidation of humoral immunity by studying antibody levels in diseased patients when compared to healthy controls (Mouton et al 1981. Ranney et al, 1982, Schonfeld & Kagan 1982.…”

mentioning

confidence: 99%

Effect of periodontal therapy on specific antibody responses to suspected periodontopathogens*

Vincent

Falkler

Cornett

et al. 1987

The effects of clinically successful periodontal therapy were studied in juvenile periodontitis (JP) and rapidly progressive periodontitis (RP) patients and compared with periodontally healthy subjects (HS). Serum samples were obtained in 35 HS prior to the study and in 12 of these subjects 3-4 years later. Serum samples were obtained from 50 JP patients initially, 9 subjects immediately following surgical therapy and 29 of these subjects 3-4 years later. RP patients provided 46 initial serum samples, 9 following therapy and 27 samples 3-4 years later. Antibody levels were determined utilizing a standardized enzyme-linked immunosorbent assay with Bacteroides gingivalis, B. ochracea, Fusobacterium nucleatum and Actinobacillus actinomycetemcomitans serving as antigens. The JP patients showed an initial rise in antibody levels immediately following therapy followed by a significant decrease in antibody levels 3 to 4 years later. The RP patients did not show an early change in antibody levels but by 3 to 4 years post-therapy, antibody levels had significantly decreased. However, during this study, the antibody levels of JP and RP patients remained significantly higher when compared with HS patients.

“…Bacteriological studies revealed that certain gram-negative bacteria are related to the etiology and pathophysioiogy of various types of periodontitis (Socransky 1977, Slots 1979, Jacob et al 1982, Tew et al 1985b, Page & Schroder 1982, Furthermore, immunological studies using various methods have been carried out, based on an assumption that local bacterial infection elicits the host immune responses being involved in the etiology of periodontal diseases (Altman 1982), Mouton et al, (1981) and Ebersole et al (1980) first applied the enzyme-linked immunosorbent assay (ELISA), which was developed by Fngvall and Perlemann (1972), to the field of periodontics. Using this technique, studies of serum antibodies against periodontopathic bacteria have been performed.…”

mentioning

confidence: 99%

Effect of periodontal treatments on serum IgG antibody titers against periodontopathic bacteria

Horibe

Watanabe

Ishikawa

1995

Serum IgG antibody titers to 7 periodontopathic bacteria in periodontitis patients were measured at the 1st visit and after various periodontal treatments with clinically successful improvement, in order to evaluate what kind of factors are associated with changes of serum antibody titers. 20 patients (10 male and 10 female from 23 to 61 years old) with adult, rapidly progressive periodontitis were enrolled in this study. All patients received initial preparation and most of them also underwent surgical procedure. After the treatments, the mean probing pocket depths decreased from 3.72 mm to 1.56 mm. Serum samples were collected from patients at the initial and final examinations. Serum IgG antibody titers against sonicated antigens of Porphyromonas gingivalis FDC 381, Prevotella intermedia ATCC 25611, Prevotella loescheii ATCC 15930, Fusobacterium nucleatum subspecies nucleatum ATCC 25586, Actinobacillus actinomycetemcomitans FDC Y4, Eikenella corrodens FDC 1073 and Capnocytophaga ochracea # M 12 were determined by enzyme-linked immunosorbent assay. The mean antibody titers to P. gingivalis and P. intermedia decreased significantly after the treatment as compared to their pretreatment levels. The antibody titer to P. gingivalis, especially, decreased in all of the patients examined. A significant relationship was found between the decreased antibody titer to P. gingivalis and the number of teeth which received periodontal surgery, as well as treatment length, and the relationship between the decreased antibody titer to P. intermedia and the number of extracted teeth was also significant. These results suggest that the changes of serum IgG titers against P. gingivalis and P. intermedia are related to the suppression of such pathogens in subgingival plague.