Serum anti-Escherichia coli antibodies and urinary β-glucuronidase for the diagnosis and control of evolution of urinary infection during pregnancy

Vinacur, Jorge; Casellas, J. Gaztambide; Rubí, Raúl A.; Oneto, Edgardo P.

doi:10.1016/0002-9378(74)90586-9

Cited by 6 publications

(1 citation statement)

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“…Although the highest concentrations of urolithin phase II metabolites were observed in urine, no studies have been conducted yet regarding the impact of these compounds on ailments associated with urinary tract disorders. Uropathogenic E. coli is the main cause of urinary tract infections, which produces significant amounts of b-glucuronidase (Vinacur et al, 1974;Helander and Dahl, 2005;McLellan and Hunstad, 2016). We have shown that standard strains of E. coli, as well as clinical isolates from patients with urinary tract infections, were capable of urolithin glucuronide cleavage.…”

Section: Discussionmentioning

confidence: 98%

Phase II Conjugates of Urolithins Isolated from Human Urine and Potential Role ofβ-Glucuronidases in Their Disposition

et al. 2017

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In recent years, many xenobiotics derived from natural products have been shown to undergo extensive metabolism by gut microbiota. Ellagitannins, which are high molecular polyphenols, are metabolized to dibenzo[,]pyran-6-one derivatives-urolithins. These compounds, in contrast with their parental compounds, have good bioavailability and are found in plasma and urine at micromolar concentrations. In vivo studies conducted for ellagitannin-containing natural products indicate their beneficial health effects toward inflammation and cancer, which are associated with the formation of urolithins. However, the great majority of in vitro experiments that have revealed the molecular mechanisms responsible for the observed effects were conducted for urolithin aglycones. These studies are thus incongruent with the results of pharmacokinetic studies that clearly indicate that glucuronide conjugates are the dominant metabolites present in plasma, tissue, and urine. The aim of this study was to isolate and structurally characterize urolithin conjugates from the urine of a volunteer who ingested ellagitannin-rich natural products, and to evaluate the potential role of -glucuronidase-triggered cleavage in urolithin disposition. Glucuronides of urolithin A, iso-urolithin A, and urolithin B were isolated and shown to be cleaved by the-glucuronidases released by neutrophils from azurophilic granules upon -formylmethionine-leucyl-phenylalanine stimulation as well as by standard strains and clinical isolates from patients with urinary tract infections. These results justify the hypothesis that the selective activation of urolithin glucuronides by -glucuronidase, which are present at high concentrations at inflammation and infection sites and in the microenvironments of solid tumors, could locally increase the concentration of bioactive urolithin aglycones.

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Section: Discussionmentioning

confidence: 98%