Serum and urine profiling by high-throughput TMT-based proteomics for the investigation of renal dysfunction in canine babesiosis

Bilić, Petra; Horvatić, Anita; Kuleš, Josipa; Gelemanović, Andrea; Ljubić, Blanka Beer; Muñoz-Prieto, Alberto; Gotić, Jelena; Žubčić, Damir; Rafaj, Renata Barić; Mrljak, Vladimir

doi:10.1016/j.jprot.2022.104735

Cited by 7 publications

(6 citation statements)

References 53 publications

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“…Acute phase proteins, such as lipopolysaccharide-binding protein (LBP), leucine rich alpha-2-glycoprotein (LRG1), LTF, paraoxonase (PON3), clusterin (CLU), histidine rich glycoprotein (HRG), ceruloplasmin (CP), alpha-1-acid glycoprotein (ORM1), inter-alpha-trypsin inhibitor heavy chain 2 and 4 (ITIH2, ITIH4), were also identified in this study. Most of them have already been previously reported in canine babesiosis 32 . This is however the first time that lactotransferrin has been reported in this disease.…”

Section: Discussionmentioning

confidence: 94%

Serum proteome profiling of naturally acquired Babesia rossi infection in dogs

Kuleš

Rubić

Farkaš

et al. 2023

Sci Rep

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Babesiosis is a disease of significant medically and veterinary importance with worldwide distribution. It is caused by intra-erythrocyte protozoal parasites, with Babesia rossi causing the most severe clinical signs of all the large Babesia parasites infecting dogs. The disease can be clinically classified into uncomplicated and complicated forms with a wide range of clinical presentations from a mild, subclinical illness to complicated forms and death. The aim of this study was to assess serum proteomic profiles from dogs with babesiosis and healthy dogs using a label-based proteomics approach. Altogether 32 dogs naturally infected with B. rossi (subdivided into 18 uncomplicated cases and 14 complicated cases of babesiosis) and 20 healthy dogs were included. There were 78 proteins with significantly different abundances between the three groups of dogs. Elucidation of proteins and pathways involved in canine babesiosis caused by B. rossi have revealed key differences associated with haemostasis, innate immune system, lipid metabolism and inflammation. Shotgun proteomic profiling allowed identification of potential serum biomarkers for differentiation of disease severity in canine babesiosis caused by B. rossi. These findings may be applicable to the study of host-parasite interactions and the development of novel therapeutic targets.

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Section: Discussionmentioning

confidence: 94%

Serum proteome profiling of naturally acquired Babesia rossi infection in dogs

Kuleš

Rubić

Farkaš

et al. 2023

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Acute phase proteins, such as lipopolysaccharide-binding protein (LBP), leucine rich alpha-2-glycoprotein (LRG1), LTF, paraoxonase (PON3), clusterin (CLU), histidine rich glycoprotein (HRG), ceruloplasmin (CP), alpha-1-acid glycoprotein (ORM1), inter-alpha-trypsin inhibitor heavy chain 2 and 4 (ITIH2, ITIH4), were also identi ed in this study. Most of them have already been previously reported in canine babesiosis 32 . This is however the rst time that lactotransferrin has been reported in this disease.…”

Section: Proteomic Pro Ling Between Control Group Uncomplicated and C...mentioning

confidence: 94%

Serum proteome profiling of naturally acquired Babesia rossi infection in dogs

Kuleš

Rubić

Farkaš

et al. 2023

Preprint

Self Cite

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Babesiosis is a disease of significant veterinary importance with worldwide distribution. It is caused by intra-erythrocyte protozoal parasites, with Babesia rossi causing the most severe clinical signs of all the large Babesia parasites infecting dogs. The disease can be clinically classified into uncomplicated and complicated forms with a wide range of clinical presentations from a mild, subclinical illness to complicated forms and death. The aim of this study was to assess serum proteomic profiles from dogs with babesiosis and healthy dogs using a label-based proteomics approach. Altogether 32 dogs naturally infected with B. rossi (subdivided into 18 uncomplicated cases and 14 complicated cases of babesiosis) and 20 healthy dogs were included. There were 78 proteins with significantly different abundances between the three groups of dogs. Elucidation of proteins and pathways involved in canine babesiosis caused by B. rossi have revealed key differences associated with haemostasis, innate immune system, lipid metabolism and inflammation. Shotgun proteomic profiling allowed identification of potential serum biomarkers for differentiation of disease severity in canine babesiosis caused by B. rossi. These findings may be applicable to the study of host-parasite interactions and the development of novel therapeutic targets.

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“…All further analyses were based on this core proteome. The proteome coverage of 398 proteins is within the range of current serum proteomic studies (21,(23)(24)(25), although modern fast scanning mass spectrometers reduce the need for depletion of high abundance proteins. The "core proteome" of 398 proteins for the present study spans several orders of quantified protein intensity with a comparable range for all 84 samples (Figure 3), highlighting the successful merging of the 10 TMT label pools used with reference label channels and the corresponding MSStatsTMT algorithms.…”

Section: Proteome Coveragementioning

confidence: 99%

Serum Proteome Profiling Identifies N-Cadherin and C-Met as Early Marker Candidates of Therapeutic Response to Neoadjuvant Chemotherapy in Breast Cancer

Steenbuck,

Cosenza-Contreras,

Fröhlich

et al. 2024

Preprint

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Breast cancer remains the most common cancer in women worldwide. Neoadjuvant chemotherapy (NACT) is often preferred to adjuvant chemotherapy to achieve tumour shrinkage, monitor response to therapy and facilitate surgical removal in the absence of metastases. In addition, there is strong evidence that pathological complete remission (pCR) is associated with prolonged survival. In this study, we sought to identify candidate markers that signal response or resistance to therapy. We present a retrospective longitudinal serum proteomic study of 22 breast cancer patients (11 with pCR and 11 with non-pCR) matched with 21 healthy controls. Serum was analysed by LC-MS/MS after depletion of abundant proteins by immunoaffinity, trypsinisation, isobaric labelling and fractionation by reversed-phase HPLC. We observed an inverse behaviour of the serum proteins c-Met and N-cadherin after the second cycle of chemotherapy with a high predictive value (AUC 0.93). More pronounced changes were observed after the 6th cycle of NACT, with significant changes in the intensity of the proteins contactin-1, centrosomal protein, sex hormone-binding globuline and cholinesterase. Our study highlights the possibility of monitoring response to NACT using serum as a liquid biopsy.

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Serum and urine profiling by high-throughput TMT-based proteomics for the investigation of renal dysfunction in canine babesiosis

Cited by 7 publications

References 53 publications

Serum proteome profiling of naturally acquired Babesia rossi infection in dogs

Serum proteome profiling of naturally acquired Babesia rossi infection in dogs

Serum proteome profiling of naturally acquired Babesia rossi infection in dogs

Serum Proteome Profiling Identifies N-Cadherin and C-Met as Early Marker Candidates of Therapeutic Response to Neoadjuvant Chemotherapy in Breast Cancer

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