Serum and urine in diabetics on diagnosis and subsequent treatment, and stable insulin dependent diabetics

Whiting, P. H.; Ross, I.S.; Borthwick, L J

doi:10.1016/0009-8981(79)90227-4

Cited by 79 publications

(26 citation statements)

References 12 publications

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“…Others have found that increased Nacetyl-(j-D-glucosaminidase excretion does appear to be associated with poor glycaemic control and microalbuminuria but not in patients with retinopathy .17 Excretion of N-acetyl-(j-D-glucosaminidase in newly diagnosed diabetic patients was reduced by treatment. 18 Previous studies in diabetic children 19 • 20 suggest that poor glycaemic control is associated with increased excretion of low molecular weight proteins. Similarly, Chiarelli and colleagues!'…”

Section: Discussionmentioning

confidence: 98%

Glomerular and Tubular Proteinuria in Type 1 (Insulin-Dependent) Diabetic Patients with and without Retinopathy

et al. 1992

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SUMMARY. We compared the urinary excretion of albumin, transferrin, N-acetyl--D-glucosaminidase and o-l-microglcbulin in 78 Type 1 (insulin-dependent) diabetic patients: 39 with retinopathy and 39 without. The two groups were matched for age, sex and duration of diabetes. The patients with retinopathy had increased excretion (median and range) of albumin [1'7 (0' 3-399·1) versus 1·0 (0' 3-116'6) mg/mmol creatinine, P<0'05j, transferrin [114'2 (4'1-37126'2) versus 33·4 (1'0-4176'7) JLg/mmol creatinine, P< 0'01] and N-acetyl-~-D-glucosaminidase [23' 8 (1'1-119'1) versus 15·0 (0'1-65 '1) JLmol/h/mmol creatinine, P

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Section: Discussionmentioning

confidence: 98%

Glomerular and Tubular Proteinuria in Type 1 (Insulin-Dependent) Diabetic Patients with and without Retinopathy

et al. 1992

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“…NAG activity was determined in the plasma samples by the method of Whiting et al (1979) as described in a previous publication (Iqbal et al, 1998). Briefly, 0.1 ml of plasma was diluted with 0.4 ml of distilled water.…”

Section: Analysis Of Nagmentioning

confidence: 99%

“…However, its levels have been found to be increased in a number of diseases, such as diabetes mellitus (Whiting et al, 1979;Severini et al, 1988), liver disease (Severini et al, 1990) and cancer (Reglero et al, 1980;Severini and Aliberti, 1994;. NAG activity has also been reported to be elevated in inflammatory conditions like arthopathies and chronic obstructive pulmonary diseases (Javed et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

N-Acetyl-β-D-glucosaminidase in acute myocardial infarction

Iqbal

Kazmi²,

Jafri³

et al. 2003

Exp Mol Med

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A bstractThe objective of the study was to investigate whether the lysosom al enzym e, N -Acetyl-β-D-glucosam inidase (NAG ) activity is increased in plasm a of patients with acute m yocardial infarction (AMI) and to determ ine if there is any association betw een plasm a levels of NAG and severity of m yocardial infarction (M I). N A G activity in plasm a w as m onitored in 69 patients with AMI and 135 norm al healthy subjects using a spectrofluorim etric m ethod. A m odified Aldrich ST elevation score w as used to gauge the severity of MI in term s of size of the infarct. Plasm a NAG levels in AMI patients and norm al healthy subjects w ere found to be 10.92±7.5 U /l and 6.8±2.2 U /l, respectively. These tw o m ean value when com pared by Student's t-test were significantly different P = 0.0001. No statistically significant differences in NAG activity w ere observed in patients in term s of gender, age, location of infarct, tim e from onset of chest pain to blood sam pling in the hospital and size of the infarct.

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“…These were dialyzed against 10 mM phosphate buffer, pH 7.0 for 4 h. NAG activity in the dialyzed samples was determined by the method of Whiting et al (1979b). This procedure involves incubation of 0.1 ml of urine diluted with 0.4 ml of distilled water and 4.5 ml of 20 mM citratephosphate buff e r, pH 4.4 containing 0.2 mM methylumbelliferyl-glucosaminidine at 37˚C.…”

Section: Analysis Of Nagmentioning

confidence: 99%

“…Its levels in urine have been shown to be elevated in a number of diseases, such as diabetes mellitus (Whiting et al, 1979a;1979b;Hanseus et al, 1983;Shimajo et al, 1987;Skrha et al, 1987;Severni et al, 1988;Watts et al, 1988), renal stone disease (Javed et al, 1992) and impaired renal function disorders (Ellis et al, 1975). More r e c e n t l y, it has been reported that urinary NAG levels are elevated in adjuvant arthritis (Franch et al, 1990) and there appears to be a positive correlation between urinary NAG excretion and severity of the disease.…”

Section: Introductionmentioning

confidence: 99%

Urinary N-acetyl-β-D-glucosaminidase in rheumatoid arthritis

Iqbal

Ali²,

Waqar³

et al. 1998

Exp Mol Med

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Excretion of urinary N-acetyl -D-g l u c o s a m i n i d a s e (NAG)and its isoenzyme patterns were studied in two groups of patients with rheumatoid arthritis (RA) and in normal control subjects. Urine samples were collected from 30 seropositive RA patients, 19 seronegative RA patients, and 15 normal healthy subjects. All the patients and normal subjects were assessed to have normal liver and kidney functions. A small portion of the urine sample was dialyzed against 0.01 M phosphate buffer, pH 7.0 and NAG activity was monitored. Mean ± SD values of urinary NAG in seropositive RA patients, in seronegative RA patients and in normal healthy subjects were found to be 4.20 ± 3.73 U/g creatinine, 2.96 ± 2.11 U/gm creatinine, and 1.71 ± 0.6 U/g creatinine, r e s p e c t i v e l y. The mean urinary NAG value in RA patients was found to be significantly higher (P < 0.05) in seropositive RA compared to the mean NAG value in normal healthy subjects and patients with seronegative RA when analyzed by one way ANOVA and Tukey-HSD test. The mean proportion of isoenzyme form B to isoenzyme form A in seropositive RA patients was also found to be significantly different (P < 0.05) from the mean proportion of these forms in normal healthy subjects and seronegative RA patients. There also appears to be a correlation between the concentration of urinary NAG and severity of the disease in seropositive RA.

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Serum and urine in diabetics on diagnosis and subsequent treatment, and stable insulin dependent diabetics

Cited by 79 publications

References 12 publications

Glomerular and Tubular Proteinuria in Type 1 (Insulin-Dependent) Diabetic Patients with and without Retinopathy

Glomerular and Tubular Proteinuria in Type 1 (Insulin-Dependent) Diabetic Patients with and without Retinopathy

N-Acetyl-β-D-glucosaminidase in acute myocardial infarction

Urinary N-acetyl-β-D-glucosaminidase in rheumatoid arthritis

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