Serum and tissue expression of neuropilin 1 in precancerous and malignant vocal fold lesions

Abstract

The study was designed to evaluate the tissue expression of NRP-1 and serum level of sNRP-1 in the same patients with intraepithelial laryngeal lesions or early staged laryngeal cancer to identify the clinical significance of these biomarkers in the diagnosis of laryngeal lesions. Material and methods A prospective analysis of tissue was performed on specimens and blood samples from 49 patients, who were admitted for surgical resection due to suspicious vocal fold lesions and were diagnosed as non-dysplasia, low-grade dysplasia, high-grade dysplasia and invasive cancers. Results ELISA was conducted on 48 blood samples. The minimum level of sNRP-1 was 0.15 ng/ml and maximum-37.71 ng/ml. The Kruskal-Wallis one-way analysis of variance revealed no differences in sNRP-1 levels between different histopathological stages of vocal fold lesions (p = 0.234). IHC was conducted in 49 tissue samples. The evaluated mean scores of NRP-1 tissue expression were compared to histopathological stage of the lesion. The Kruskal-Wallis one-way analysis of variance revealed no differences in NRP-1 tissue expression between different histopathological stages of vocal fold lesions (p = 0.536). The correlation of tissue NRP-1 expression and serum levels of NRP-1 within analyzed group was insignificant. The Spearman's rank correlation coefficient was 0.076 (p = 0.606).

Clinical impact of soluble Neuropilin-1 in ovarian cancer patients and its association with its circulating ligands of the HGF/c-MET axis

“…Principal component analysis using sNRP-1, sHGF and sMET levels. controls or patients with benign vocal cord lesions, respectively (22,33).…”

“…The clinical data from the patients are summarized in Table 1. Tumor staging was documented according to the Fedeŕation Internationale de Gynećologie et d'Obstetrique (FIGO) (22), revised in 2014 (23). Hence, the revised version was used for all patients who underwent primary surgery from 2014 onwards.…”

miRNAs Involved in Esophageal Carcinogenesis and miRNA-Related Therapeutic Perspectives in Esophageal Carcinoma

“…MiR-124 is involved in EC progression by regulating the expression of its target gene NRP1 [ 59 ]. NRP1 is a receptor capable of binding many growth factors, such as TGF-β, PDGF and VEGF [ 127 ]. In EC, the down-regulation of miR-124 is responsible for NRP-1 overexpression [ 59 ] and is linked to poor prognosis, high clinical stage and cell migration [ 59 ].…”

New Biosensor for Determination of Neuropilin-1 with Detection by Surface Plasmon Resonance Imaging

“…However, detecting neuropilin-1 in tissue is invasive, complex and expensive compared with measuring the level of circulating NRP-1 in body fluids. In recent years, increasing numbers of studies on the detection of plasma NRP-1 levels have been published [15,[25][26][27]. There are also reports of NRP-1 determination in urine [28].…”