Serum and salivary adiponectin dynamics in septic and non-septic systemic inflammation in a canine model

Dąbrowski, Roman; Wdowiak, Anna; Contreras-Aguilar, María Dolores; Franco‐Martínez, Lorena; Muñoz-Prieto, Alberto; Szczubiał, Marek; Martı́nez-Subiela, Silvia; Cerón, José J.

doi:10.1016/j.vetimm.2019.109961

Cited by 2 publications

(1 citation statement)

References 17 publications

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“…In dogs, saliva has been usefully employed for the diagnosis of infectious (10)(11)(12) and metabolic (13) diseases, among others. Saliva composition is also known to be modulated in canine pyometra because increased and decreased concentrations of adenosine deaminase and adiponectin, respectively-biomarkers related to of inflammation-were found in bitches with pyometra in comparison to healthy ones (14,15). Therefore, a detailed overview of the possible changes in salivary protein composition could improve the understanding of the physiopathology of canine pyometra, as well as point out possible biomarkers of the disease.…”

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confidence: 99%

Changes in the Salivary Proteome Associated With Canine Pyometra

et al. 2020

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The present study evaluated for the first time changes in the saliva proteome in bitches with pyometra through a high-throughput quantitative proteomic analysis. The aims were to explore whether saliva composition could reflect the physiopathological changes occurring in canine pyometra and to identify potential biomarkers of the disease. Saliva samples from six healthy (H) and six bitches with pyometra (P) were analyzed using tandem mass tags-based approach. Additionally, 15 samples were used for the validation of changes in haptoglobin (Hp) concentration in saliva of dogs with pyometra. Proteomic analysis quantified 707 proteins in saliva. Comparison of the two groups revealed 16 unique proteins significantly modulated in saliva, with S100A calcium-binding protein 12 (S100A12), vimentin, and Hp the most up-regulated in canine pyometra. According to PANTHER (Protein Analysis Through Evolutionary Relationships) classification tool, these proteins are mainly related to proinflammatory mediators, acute-phase proteins, and sepsis. In conclusion, it can be stated that there are changes in various proteins in saliva in canine pyometra reflecting different physiopathological changes occurring in this disease. These proteins could be a source of potential non-invasive biomarkers for this disease that should be confirmed in future studies.

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