Serum and intracellular magnesium concentrations in intoxicated chronic alcoholic and control subjects

Princi, Tanja; Artero, Mary; Malusà, Noelia; Uxa, Laura; Livia, Vincenzo; Reina, Giuseppe

doi:10.1016/s0376-8716(97)00037-9

Cited by 8 publications

(8 citation statements)

References 17 publications

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“…In human medicine, the need for a more sensitive marker of magnesium deficiency was identified years ago and different methods for the determination of the intracellular magnesium concentration in multiple tissues, and blood cells have been developed (Huijgen et al, 1998;Reinhart, Marx, Haas, & Desbiens, 1987;Schwinger, Antoni, & Guder, 1987). Intracellular electrolyte measurements have been performed in platelets (Niemela, Csako, Bui, & Elin, 1997;Yoshimura et al, 1998), lymphocytes (Delva et al, 2006;Princi et al, 1997) and erythrocytes (Basso, Ubbink, & Delport, 2000). Values obtained by these techniques better reflect the actual situation in the cell, like in skeletal (Princi et al, 1997), vascular smooth muscle and cardiac muscle (Niemela et al, 1997) than does the serum magnesium concentration.…”

Section: Discussionmentioning

confidence: 99%

“…Intracellular electrolyte measurements have been performed in platelets (Niemela, Csako, Bui, & Elin, 1997;Yoshimura et al, 1998), lymphocytes (Delva et al, 2006;Princi et al, 1997) and erythrocytes (Basso, Ubbink, & Delport, 2000). Values obtained by these techniques better reflect the actual situation in the cell, like in skeletal (Princi et al, 1997), vascular smooth muscle and cardiac muscle (Niemela et al, 1997) than does the serum magnesium concentration. Of these techniques, platelet and lymphocyte magnesium measurements are time-consuming and have not been established as a routine procedure in human medicine.…”

Section: Discussionmentioning

confidence: 99%

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Intracellular free magnesium concentration in healthy horses

Winter

Sponder

Merle

et al. 2018

Animal Physiology Nutrition

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Equine metabolic syndrome (EMS) is a worldwide disease in horses that parallels human diabetes mellitus type 2. In both diseases, patients show an altered peripheral insulin sensitivity as a key feature. In humans, multiple studies have demonstrated the beneficial effect of magnesium supplementation on insulin sensitivity. However, serum magnesium levels vary and are therefore not a reliable indicator of the patients' magnesium status. Determining the intracellular free magnesium concentration appears to be a more sensitive diagnostic indicator. In this study, the free intracellular magnesium concentration was measured using mag-fura 2 spectrophotometry in blood lymphocytes in 12 healthy, non-obese horses at 9 a.m., 12 a.m. and 4 p.m. to establish reference ranges according to a protocol designed for human blood lymphocytes. Additionally, the serum magnesium concentration was measured. In all horses, the total serum magnesium concentration was within the reference range. The mean free magnesium concentration in blood lymphocytes of all horses was 0.291 ± 0.067 mmol/L with no significant difference between the time points. The reference range for the free intracellular magnesium concentration in equine lymphocytes was set at 0.16-0.42 mmol/L. The established values are slightly lower than those in healthy humans. The designed protocol for the measurement of the intracellular free magnesium concentration might be an excellent research tool to assess the cellular magnesium status and to reliably diagnose an altered magnesium homeostasis in EMS. Further studies shall elucidate possible alterations in cellular magnesium status in horses with EMS.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Intracellular free magnesium concentration in healthy horses

Winter

Sponder

Merle

et al. 2018

Animal Physiology Nutrition

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“…The dose-related increase in both alcohol. In earlier studies small di OE erences in urine Ca and Mg excretion after oral Mg loading serum Mg have been found in chronic alcoholics is an even stronger argument in favour of a as compared with controls [15,26,27 ] although disorder of Ca and Mg reabsorption at the some studies have found no di OE erences [28]. In tubular level in consumers, particularly if conview of the above, it cannot be excluded that sidering the opposite changes in urine Ca there could also have been small di OE erences at excretion after oral Mg loading in nonthe intracellular level in this study, although consumers.…”

Section: Beforementioning

confidence: 97%

Moderate alcohol consumption and urinary excretion of magnesium and calcium

Rylander¹,

Mégevand²,

Lasserre³

et al. 2001

Scandinavian Journal of Clinical and Laboratory Investigation

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The aim of this study was to evaluate the magnesium (Mg) status of male subjects consuming moderate amounts of alcohol (n = 14) in comparison with that of a group of non-consumers of alcohol (n = 10). Plasma ionized Mg levels and total erythrocyte Mg content were determined as well as the excretion of Mg in urine before and after an oral loading test. Intake of Mg via food and water was estimated using a one-week dietary records. The results showed a significantly higher, alcohol dose-related excretion of Mg and Ca (calcium) in the urine after the oral Mg load among consumers of alcohol. Although the study is based on a small number of subjects with differences in smoking habits, it is suggested that alcohol consumption even in moderate amounts could contribute to Mg deficiency.

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“…Tissue biopsies would be a more precise, but also more invasive procedure. The intracellular magnesium concentration [Mg 2+ ]i in blood lymphocytes (Princi et al 1997, Delva et al 2006, erythrocytes (Basso et al 2000) or platelets (Niemela et al 1997, Yoshimura et al 1998) is similar to the concentration in the tissue. Therefore it has been used as a preferred marker of the magnesium metabolism (Princi et al 1997).…”

Section: Introductionmentioning

confidence: 99%

“…The intracellular magnesium concentration [Mg 2+ ]i in blood lymphocytes (Princi et al 1997, Delva et al 2006, erythrocytes (Basso et al 2000) or platelets (Niemela et al 1997, Yoshimura et al 1998) is similar to the concentration in the tissue. Therefore it has been used as a preferred marker of the magnesium metabolism (Princi et al 1997). Methods to determine blood cell [Mg 2+ ]i (Reinhart et al 1987, Schwinger et al 1987 have been used in human medicine since the 1980s and reference values for [Mg 2+ ]i in healthy horses have previously been established (Winter et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Relationship between intracellular free magnesium concentration and the degree of insulin resistance in horses with equine metabolic syndrome

Winter¹,

Müller²,

Sponder³

et al. 2020

PHK

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Magnesium metabolism, especially the intracellular magnesium concentration [Mg 2+ ]i, plays an important role in human diabetes mellitus. The aim of this study was to evaluate [Mg 2+ ]i in horses with insulin resistance and to examine a possible association with other markers of insulin dysregulation. The study included 38 horses with a positive combined glucose insulin tolerance test (CGIT) and phenotypic signs of EMS. Baseline glucose (G 0), glucose curve, baseline insulin (I 0), insulin after 45 minutes (I 45), total serum magnesium concentration and [Mg 2+ ]i were measured. The reciprocal inverse square of insulin (RISQI), the modified insulin-to-glucose ratio (MIRG) and the insulin difference (I 45-0) were calculated. A t-test for independent samples was used to compare [Mg 2+ ]i in healthy and EMS horses. Linear regression analyses with uni-and multivariable models were calculated to evaluate the influence of [Mg 2+ ]i on all other blood and clinical parameters. Compared to healthy controls, [Mg 2+ ]i was significantly lower in horses with EMS (P = 0.015). Univariable linear regression models showed significant associations between I 0 (P = 0.004, b = 0.006), I 45 (P = 0.006, b = 0.001), I 45-0 (P = 0.008, b = 0.001), RISQI (P = 0.009, b =-0.158), MIRG (P = 0.001, b = 0.017) and [Mg 2+ ]i. The multivariable model revealed that MIRG significantly influenced [Mg 2+ ]i whereas the other factors had no additional influence. Horses with EMS exhibited an intracellular magnesium deficit that can be partially reversed by increased secretion of insulin. These findings substantiate the crucial role for insulin in regulating [Mg 2+ ]i and its relevance in EMS.

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Serum and intracellular magnesium concentrations in intoxicated chronic alcoholic and control subjects

Cited by 8 publications

References 17 publications

Intracellular free magnesium concentration in healthy horses

Intracellular free magnesium concentration in healthy horses

Moderate alcohol consumption and urinary excretion of magnesium and calcium

Relationship between intracellular free magnesium concentration and the degree of insulin resistance in horses with equine metabolic syndrome

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