Serum and intestinal isotype antibody responses to Wa human rotavirus in gnotobiotic pigs are modulated by maternal antibodies.

Parre√±o, V; Hodgins, Doug; Arriba, L de; Kang, Shien-Young; Yuan, Lijuan; Ward, Lucy A.; Ty, Tsu; Saif, Linda J.

doi:10.1099/0022-1317-80-6-1417

Cited by 60 publications

(70 citation statements)

References 47 publications

(27 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…IgM, IgG, and IgA HRV antibody geometric mean titers (GMTs) and total IgA concentrations in serum were measured by enzyme-linked immunosorbent assay (ELISA) pre-virHRV inoculation (PFMTD9 or PFMTD10/PID0), post-virHRV inoculation (PID9), and at the termination of the experiment (PID14) as described previously (16,21,74). The same parameters were measured in the small intestinal contents at PID14.…”

Section: Methodsmentioning

confidence: 99%

Protein Malnutrition Alters Tryptophan and Angiotensin-Converting Enzyme 2 Homeostasis and Adaptive Immune Responses in Human Rotavirus-Infected Gnotobiotic Pigs with Human Infant Fecal Microbiota Transplant

Fischer

Kandasamy

Paim

et al. 2017

Clin Vaccine Immunol

View full text Add to dashboard Cite

Malnutrition leads to increased morbidity and is evident in almost half of all deaths in children under the age of 5 years. Mortality due to rotavirus diarrhea is common in developing countries where malnutrition is prevalent; however, the relationship between malnutrition and rotavirus infection remains unclear. In this study, gnotobiotic pigs transplanted with the fecal microbiota of a healthy 2-monthold infant were fed protein-sufficient or -deficient diets and infected with virulent human rotavirus (HRV). After human rotavirus infection, protein-deficient pigs had decreased human rotavirus antibody titers and total IgA concentrations, systemic T helper (CD3 ϩ CD4 ϩ ) and cytotoxic T (CD3 ϩ CD8 ϩ ) lymphocyte frequencies, and serum tryptophan and angiotensin I-converting enzyme 2. Additionally, deficientdiet pigs had impaired tryptophan catabolism postinfection compared with sufficient-diet pigs. Tryptophan supplementation was tested as an intervention in additional groups of fecal microbiota-transplanted, rotavirus-infected, sufficientand deficient-diet pigs. Tryptophan supplementation increased the frequencies of regulatory (CD4 ϩ or CD8 ϩ CD25 ϩ FoxP3 ϩ ) T cells in pigs on both the sufficient and the deficient diets. These results suggest that a protein-deficient diet impairs activation of the adaptive immune response following HRV infection and alters tryptophan homeostasis.

show abstract

Section: Methodsmentioning

confidence: 99%

Protein Malnutrition Alters Tryptophan and Angiotensin-Converting Enzyme 2 Homeostasis and Adaptive Immune Responses in Human Rotavirus-Infected Gnotobiotic Pigs with Human Infant Fecal Microbiota Transplant

Fischer

Kandasamy

Paim

et al. 2017

Clin Vaccine Immunol

View full text Add to dashboard Cite

show abstract

“…The ELISPOT assay for measuring the total immunoglobulin-secreting cell (IgSC) response and data reporting followed a previously described protocol (16). To determine the HRV-specific IgA and IgG antibody titers in serum and intestinal contents, isotypespecific ELISA was performed according to the protocol previously described (22,23). Total IgM, IgA, and IgG antibody titers in serum and intestinal contents were assessed by following an established ELISA protocol (24).…”

Section: Methodsmentioning

confidence: 99%

Dietary Rice Bran Protects against Rotavirus Diarrhea and Promotes Th1-Type Immune Responses to Human Rotavirus Vaccine in Gnotobiotic Pigs

Yang

Wen

Tin

et al. 2014

Clin Vaccine Immunol

View full text Add to dashboard Cite

bRice bran (RB) contains a distinct stoichiometry of phytochemicals that can promote gut mucosal immune responses against enteric pathogens. The effects of RB on rotavirus diarrhea and immunogenicity of an attenuated human rotavirus (HRV) vaccine were evaluated in gnotobiotic pigs. The four treatment groups studied were RB plus vaccine, vaccine only, RB only, and mock control. Pigs in the RB groups were fed the amount of RB that replaced 10% of the pigs' total daily calorie intake from milk starting from 5 days of age until they were euthanized. Pigs in the vaccine groups were orally inoculated with two doses of the attenuated HRV vaccine. A subset of pigs from each group was orally challenged with the homologous virulent HRV on postinoculation day 28. Diarrhea and virus shedding were monitored daily from postchallenge day 0 to day 7. RB feeding significantly protected against diarrhea upon virulent HRV challenge and enhanced the protective rate of the vaccine against rotavirus diarrhea. Consistent with protection, RB significantly increased gamma interferon (IFN-␥)-producing CD4؉ and CD8 ؉ T cell responses in intestinal and systemic lymphoid tissues. Furthermore, RB also increased the number of total IgM-and IgA-secreting cells, total serum IgM, IgG, and IgA titers, and HRV-specific IgA titers in intestinal contents. RB reduced the numbers of intestinal and systemic HRV-specific IgA and IgG antibody-secreting cells and reduced serum HRV-specific IgA and IgG antibody titers before the challenge. These results demonstrate clear beneficial effects of RB in protection against rotavirus diarrhea and stimulation of nonspecific and HRV-specific immune responses, as well as its biased Th1-type adjuvant effect for the vaccine. Rice bran (RB), a globally accessible, abundant, and underutilized agricultural by-product, has a distinct stoichiometry of bioactive compounds, phytochemicals, and minerals (1). It has been studied for bioactive functions such as the prevention and treatment of chronic diseases, growth of beneficial intestinal microbes, induction of mucosal and systemic immune responses, and protection against enteric pathogens (2-5). Thus, this agricultural by-product represents a promising and practical diet-based solution for increasing the innate resistance against enteric pathogens that cause diarrhea. In particular, because of its immune stimulatory functions, it can be potentially used as a vaccine adjuvant for enteric pathogen infections. Given that RB can support colonization of gut probiotics (e.g., Lactobacillus spp.), enhance mucosal IgA production (2), and significantly reduce the enteric burden of Salmonella infection in mice (5), continued investigation of the mechanisms of dietary RB in its protection against viral pathogens that cause significant global morbidity and mortality (e.g., rotavirus) is warranted.Previous studies have shown the immunomodulatory effects of RB on both innate and adaptive immunity in vitro and in vivo (4). RB oil enhanced T and B lymphocyte proliferation, production of Th...

show abstract

“…Serum from pigs that did not shed virus and serum and intestinal contents from pigs inoculated intravenous with virulent HRV were tested by antibody-ELISA as previously described for seroconversion to immunoglobulin M (IgM), IgA, and IgG antibodies to HRV (1,24,32).…”

Section: Serum Infectivitymentioning

confidence: 99%

Viremia and Nasal and Rectal Shedding of Rotavirus in Gnotobiotic Pigs Inoculated with Wa Human Rotavirus

Azevedo

Yuan

Jeong

et al. 2005

J Virol

View full text Add to dashboard Cite

Group A rotaviruses are the most common cause of dehydrating diarrhea in infants and young children worldwide, with more than 2 million hospitalizations yearly and approximately 440,000 deaths. It is estimated that 82% of rotavirus deaths occur in children in the poorest countries (23). Rotavirus transmission occurs mainly by the fecal-oral route, although respiratory transmission has been suggested to occur (7).Rotavirus infection was thought to be limited to the gastrointestinal tract. However, respiratory symptoms and rotavirus shedding in nasopharyngeal secretions have been reported in children with and without gastrointestinal symptoms (19,26,42). Rotavirus antigen was detected in the lung of 1 of 13 experimentally infected 3-week-old conventional pigs at postinoculation day 2 (30) and in liver and kidney specimens from immunodeficient children (9). Rotavirus RNA has also been detected in cerebrospinal fluid and blood of children with central nervous system disease (20,34). Recently, Blutt and colleagues (2) detected rotavirus antigenemia in the serum of children, mice, rabbits, and calves. They further demonstrated that serum from infected mice induced rectal rotavirus antigen shedding after oral inoculation of rotavirus-negative adult mice with the serum. Previously, another enteric virus, the porcine enteric calicivirus (PEC), has also been associated with transient viremia (infectious virus in serum) after oral inoculation of gnotobiotic pigs (11).We choose gnotobiotic pigs because they constitute an animal model of HRV-induced disease. Their gastrointestinal tract physiology and their development of mucosal immunity resemble that of humans. These similarities with HRV infections of infants allow us to establish correlations which could be applied for rotavirus vaccine development (14,25) The question addressed in our study was whether an attenuated human rotavirus and virulent HRV causes upper respiratory tract infections or viremia in naïve neonatal gnotobiotic pigs after various routes of inoculation. In this study we evaluated nasal and rectal virus shedding and viremia after oral, intranasal, feeding tube (gavage), and intravenous inoculation of neonatal gnotobiotic pigs with the Wa strain of attenuated HRV or virulent HRV. The presence of infectious virus in serum of gnotobiotic pigs after oral inoculation with Wa HRV was also investigated by oral and intravenous reinoculation of gnotobiotic pigs with a pool of the HRV-positive sera. MATERIALS AND METHODSVirus. The attenuated cell culture-adapted Wa strain HRV (P1A [8]G1), derived from the 27th HRV passage in African Green monkey kidney cells (MA104) and the virulent Wa HRV from pooled intestinal contents of gnotobiotic pigs were used for inoculation of the gnotobiotic pigs at doses of 5 ϫ 10 7

show abstract

Serum and intestinal isotype antibody responses to Wa human rotavirus in gnotobiotic pigs are modulated by maternal antibodies.

Cited by 60 publications

References 47 publications

Protein Malnutrition Alters Tryptophan and Angiotensin-Converting Enzyme 2 Homeostasis and Adaptive Immune Responses in Human Rotavirus-Infected Gnotobiotic Pigs with Human Infant Fecal Microbiota Transplant

Protein Malnutrition Alters Tryptophan and Angiotensin-Converting Enzyme 2 Homeostasis and Adaptive Immune Responses in Human Rotavirus-Infected Gnotobiotic Pigs with Human Infant Fecal Microbiota Transplant

Dietary Rice Bran Protects against Rotavirus Diarrhea and Promotes Th1-Type Immune Responses to Human Rotavirus Vaccine in Gnotobiotic Pigs

Viremia and Nasal and Rectal Shedding of Rotavirus in Gnotobiotic Pigs Inoculated with Wa Human Rotavirus

Contact Info

Product

Resources

About