Serum and follicular fluid levels of soluble Fas, soluble Fas ligand and apoptosis of luteinized granulosa cells in PCOS patients undergoing IVF

Önalan, Göğşen; Selam, Belgin; Baran, Yusuf; Cıncık, Mehmet; Önalan, Reşide; Gündüz, Ufuk; Ural, Ali Uğur; Pabuçcu, Recai

doi:10.1093/humrep/dei068

Cited by 43 publications

(33 citation statements)

References 23 publications

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“…3), indicates significant apoptosis in Groups A and B. Apoptotic changes in the present study are further supported by the significant increase in DNA damage observed in Group A and B compared to Group C. These findings are in agreement with reports of Saito et al [2002] where they have documented increased levels of apoptotic cells in endometriosis. In another study, excessive luteinized DNA fragmentation in GC of PCOS patients undergoing IVF cycles is documented [Onalan et al 2005]. IVF outcome parameters including fertilization rate, good quality embryo formation rate, and pregnancy outcome were adversely affected in Group B (Table 2).…”

Section: Discussionmentioning

confidence: 93%

Oxidative status in granulosa cells of infertile women undergoing IVF

Karuputhula

Chattopadhyay

Chakravarty

et al. 2013

Systems Biology in Reproductive Medicine

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Studies on elevated reactive oxygen species (ROS) levels in granulosa cells (GC) and its subsequent effect on fertilization are limited. Oxidative stress (OS) mediated alterations in GC of infertile women undergoing in vitro fertilization (IVF) and embryo transfer (ET) was investigated. GC were obtained from 28 women with endometriosis (Group A), 26 women with polycystic ovary syndrome (PCOS) (Group B), and 32 women with tubal factor infertility (Group C). GC characteristics including cell count, viability, morphology and number of oocytes retrieved, and oocyte quality were assessed. OS parameters such as ROS, mitochondrial membrane potential (MMP), and DNA fragmentation were also studied and IVF outcome parameters assessed. An ∼20 fold increase in GC ROS generation was observed in Group B as compared to Group C. Though not as high as Group B, Group A also showed significantly high ROS levels compared with Group C. More than 100-fold decrease in MMP in Group B compared with Group C was observed. A similar trend was observed in Group A, where MMP decreased 7 fold. Significant apoptosis was evident in Groups A and B supported by depolarization of MMP and significant increase in DNA damage. IVF outcome parameters including fertilization rate, good quality embryo formation rate, and pregnancy outcome were adversely affected in Group B. It is hypothesized that ∼20 fold increase in ROS generation in GC of PCOS women plays an adverse role in affecting the IVF success rate. It was of note that the IVF outcome parameters of women with endometriosis were not affected.

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Section: Discussionmentioning

confidence: 93%

Oxidative status in granulosa cells of infertile women undergoing IVF

Karuputhula

Chattopadhyay

Chakravarty

et al. 2013

Systems Biology in Reproductive Medicine

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“…They think that low levels in this apoptotic marker imply increased apoptosis and possibly a downregulation of the immune system of patients. Onalan et al reported that low levels of serum sFas in patients with PCOS compared to male factor infertility group [20]. But they didn't found any significant difference in terms ofFF of sFas and sFasL, serum sFasL in these groups.…”

Section: Discussionmentioning

confidence: 93%

Changes of sFas and sFasL, oxidative stress markers in serum and follicular fluid of patients undergoing IVF

Pekel

Gönenç

Turhan

et al. 2014

J Assist Reprod Genet

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Purpose The Fas-Fas Ligand interaction is one of the essential events for the induction of apoptosis whereas the exact role of their soluble forms in the reproductive system is still not fully understood. Also oxidative stress in the pathogenesis of infertility causing diseases in women and has been suggested as one of the important factors that negatively affect IVF outcome. In this study, our aim was to evaluate serum and follicular fluid levels of soluble Fas soluble Fas Ligand, malondialdehyde, superoxide dismutase and total antioxidant capacity in patients undergoing IVF and compared with controls. Methods This study included 109 patients. Patients were classified as unexplained infertility (N=31), PCOS (N=19), tubal factor (N=9) and endometriosis (N=10) and compared with male factor infertility (N=40) that was the control group. sFas and sFasL levels were measured by immunoassay method. MDA, SOD and TAC levels were measured by colorimetric method. Results Patients with unexplained infertility, PCOS and tubal factor had significantly lower sFas levels compared with their controls (respectively, p<0.01, p<0.05, p<0.05). However, SOD activity in unexplained infertility, PCOS and endometriosisgroupswere significantly higher than control group (p<0.01).Decreased follicular fluid TAC levels were found in all patient groups compared with controls (respectively, p<0.01, p<0.05, p<0.01, p<0.01).Patients with tubal factor had significantly higher serum sFasL (p<0.05), but lower follicular fluid sFasL levels (p<0.05) compared with unexplained infertility. Tubal factor and endometriosis groups had lowerfollicular fluid TAC levels compared to unexplained infertility and PCOSgroups (p<0.01). Conclusion(s) In this study, serum and follicular fluid sFas levels were decreased and antioxidant activity was impaired in infertility, possibly implying increased apoptosis. Especially in unexplained infertility group changes in this parametres more remarkable.

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“…Cumulus and granulosa cells are the major source of estradiol. For PCOS patients, the apoptosis level is increased in granulosa cells [26]. Moreover, PCOS granulosa cells, unlike normal granulosa cells, show a limited capacity to synthesize progesterone, either spontaneously or in response to follicle-stimulating hormone (FSH) stimulation [27].…”

Section: Introductionmentioning

confidence: 99%

Aberrant expression of long noncoding RNAs in cumulus cells isolated from PCOS patients

Huang

Hao

Bao

et al. 2015

J Assist Reprod Genet

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Purpose To describe the long noncoding RNA (lncRNA) profiles in cumulus cells isolated from polycystic ovary syndrome (PCOS) patients by employing a microarray and in-depth bioinformatics analysis. This information will help us understand the occurrence and development of PCOS. Methods In this study, we used a microarray to describe lncRNA profiles in cumulus cells isolated from ten patients (five PCOS and five normal women). Several differentially expressed lncRNAs were chosen to validate the microarray results by quantitative RT-PCR (qRT-PCR). Then, the differentially expressed lncRNAs were classified into three subgroups (HOX loci lncRNA, enhancer-like lncRNA, and lincRNA) to deduce their potential features. Furthermore, a lncRNA/mRNA co-expression network was constructed by using the Cytoscape software (V2.8.3, http://www.cytoscape.org/). Results We observed that 623 lncRNAs and 260 messenger RNAs (mRNAs) were significantly up-or down-regulated (≥2-fold change), and these differences could be used to discriminate cumulus cells of PCOS from those of normal pat i e n t s . F i v e d i f f e r e n t i a l l y e x p r e s s e d l n c R N A s (XLOC_011402, ENST00000454271, ENST00000433673, ENST00000450294, and ENST00000432431) were selected to validate the microarray results using quantitative RT-PCR (qRT-PCR). The qRT-PCR results were consistent with the microarray data. Further analysis indicated that many differentially expressed lncRNAs were transcribed from chromosome 2 and may act as enhancers to regulate their neighboring protein-coding genes. Forty-three lncRNAs and 29 mRNAs were used to construct the coding-non-coding gene coexpression network. Most pairs positively correlated, and one mRNA correlated with one or more lncRNAs. Conclusions Our study is the first to determine genome-wide lncRNA expression patterns in cumulus cells isolated from PCOS patients by microarray. The results show that clusters of lncRNAs were aberrantly expressed in cumulus cells of PCOS patients compared with those of normal women, which revealed that lncRNAs differentially expressed in PCOS and normal women may contribute to the occurrence of PCOS and affect oocyte development.

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Serum and follicular fluid levels of soluble Fas, soluble Fas ligand and apoptosis of luteinized granulosa cells in PCOS patients undergoing IVF

Cited by 43 publications

References 23 publications

Oxidative status in granulosa cells of infertile women undergoing IVF

Oxidative status in granulosa cells of infertile women undergoing IVF

Changes of sFas and sFasL, oxidative stress markers in serum and follicular fluid of patients undergoing IVF

Aberrant expression of long noncoding RNAs in cumulus cells isolated from PCOS patients

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