Serum and exosomal miR‐122 and miR‐199a as a biomarker to predict therapeutic efficacy of hepatitis C patients

Jiao, Xiaoyang; Fan, Zhicheng; Chen, Huanzhu; He, Ping; Li, Yazhen; Zhang, Qiaoxin; Ke, Changwen

doi:10.1002/jmv.24829

Cited by 34 publications

(26 citation statements)

References 31 publications

(67 reference statements)

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“…However, serum exosome-derived miRNAs are different from the total serum miRNAs. A previous study determined that there were no correlations between serum miR-126 and exosomal miR-126 (28), and another study demonstrated that serum miR-122 and miR-199a were potential biomarkers that reflect antiviral therapy efficacy in patients with hepatitis C (29); however, to the best of our knowledge, there is no evidence that exosomal miR-122 and miR-199a have the same predictive effect.…”

Section: Characteristicmentioning

confidence: 82%

Circulating exosomal miR‑125a‑5p as a novel biomarker for cervical cancer

Huang³

et al. 2020

Oncol Lett

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Exosomal microRNAs (miRs/miRNAs) have been reported to be associated with cervical cancer. The aim of the present study was to investigate circulating exosomal miRNA as a biomarker for cervical cancer diagnosis. In the present study, samples from 6 patients with cervical cancer and 6 healthy control subjects were retrieved for exosomal RNA-sequencing. The results revealed that a total of 39 miRNAs were differentially expressed between patients with cervical cancer and healthy controls (P<0.001; fold-change >2.0). Exosomal miR-125a-5p was further quantified in plasma from 60 subjects, which included 22 healthy individuals and 38 patients with cervical cancer. miR-16a-5p served as the reference miRNA for quantitative PCR analysis of exosomal miR-125a-5p in patients with cervical cancer and healthy individuals. The results revealed that exosomal miR-125a-5p expression levels in the patients with cervical cancer were significantly lower than those in the healthy controls (P<0.001). Receiver operating characteristic (ROC) curve analyses were performed and the results revealed that the level of plasma exosomal miR-125a-5p was a potential marker for differentiating between non-cervical cancer and cervical cancer, with an ROC area under the curve of 0.7129. At the cut-off value of 2.537 for miR-125a-5p, cervical cancer diagnostic sensitivities and specificities were 59.1 and 84.2%, respectively. The present study provides confirmation that exosomal miR-125a-5p could potentially serve as a biomarker for cervical cancer diagnosis. The present study involved only a small number of clinical samples; more samples are required to support the conclusions of the present study.

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Section: Characteristicmentioning

confidence: 82%

Circulating exosomal miR‑125a‑5p as a novel biomarker for cervical cancer

Huang³

et al. 2020

Oncol Lett

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“…82–87 For example, serum exosomal miR-122, miR-134, miR-424-3p, miR629-5p, and miR-199a are candidate biomarkers for HCV. 82–84 and serum exosomal miR-214 is a potential biomarker for liver fibrosis. 85 Human biliary EVs contain abundant miR species, of which miR-191, miR-486-3p, and miR-1274b can be used to diagnose cholangiocarcinoma (CCA).…”

Section: Evs As Potential Biomarkers For Liver Diseasesmentioning

confidence: 99%

Decoding the role of extracellular vesicles in liver diseases

2017

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Cell-to-cell communication is a fascinating process that is essential for maintaining tissue and whole-body homeostasis. Extracellular vesicles (EVs) are cell-derived membrane-bound nanoparticles that are a means of communication between cells. Accumulating evidence indicates that EVs can render either beneficial or harmful outcomes, depending on the specific cargos (e.g. proteins, lipids, RNAs) transferred between cells. EVs also have great value as diagnostic and prognostic markers of disease because they are present in a variety of biological fluids and carry bioactive molecules from their cells or tissues of origin. Liver cells can both release and receive EVs derived from other cells and emerging evidence indicates that liver EVs play important roles in the pathogenesis of various liver diseases, including liver cancer, viral hepatitis, non-alcoholic fatty liver disease, and alcoholic liver disease. This review provides an overview of the biogenesis and secretion of EVs and summarizes the most recent advances in understanding the role of EVs in liver physiology and diseases. Additionally, we discuss potential applications of liver EVs as biomarkers and in therapeutic approaches to treat liver diseases.

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“…4a). Previous studies have shown that during HBV infection, circulating miR-122 levels correlate with liver damage [21] while liver miR-122 levels significantly decrease exhibiting a reverse relationship with inflammation and viral load [21]. Lower HBV DNA levels in both HBsAg carriers and acute HBV patients infected with HEV suggests lowering of HBV replication.…”

Section: Discussionmentioning

confidence: 97%

“…These miRNAs exhibit a consistent expression profile among healthy individuals. miR-122, the most abundant hepatic microRNA, has been evaluated mainly in chronic hepatitis B virus (HBV), hepatitis C virus (HCV) infections and hepatocellular carcinoma (HCC) [17][18][19][20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Circulating miR-122 levels in self-recovering hepatitis E patients

Haldipur

Arankalle

2019

ExRNA

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Background: Hepatitis E (HE) is prevalent in developing countries in both epidemic and sporadic forms and is characterized by high mortality during pregnancy. miR-122, the major hepatic microRNA has been shown to be modulated during liver diseases. Lack of data in HE led to investigations in non-pregnant (NPR) and pregnant (PR) patients. Results: Self-recovering NPR patients and pregnant women presenting with clinical (PR-acute) or subclinical (PR-SC) HE and respective healthy controls were studied. Serum samples were tested for miR-122 levels using qRT-PCR. Acute-phase, NPR patients circulated lower miR-122 levels, reducing further during convalescence. In contrast to previous reports, circulating miR-122 levels did not correlate with serum aminotransferase (ALT). In PR-acute patients, miR-122 levels were significantly lower that reflected pregnancy status and not HEV effect. In PR-SC patients, miR-122 levels were lower than the pregnant controls and reduced further when examined one month apart. A pregnant, fulminant HE patient circulated very high miR-122 levels that increased further during convalescence. Correlation analysis of miR-122 and circulating cytokines showed moderate correlation with CCL2 (subclinical, pregnant) and IL-6 (NPR). This first report revealed downregulation of circulating miR-122 levels in self-recovering NPR-acute patients despite liver damage (raised serum ALT) suggestive of alternate mechanism of secretion in blood. Conclusion: HEV infection during pregnancy led to differential modulation of serum miR-122 levels that correlated with clinical presentation. Utility of miR-122 as a prognostic marker for severe disease during pregnancy needs to be evaluated.

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Serum and exosomal miR‐122 and miR‐199a as a biomarker to predict therapeutic efficacy of hepatitis C patients

Cited by 34 publications

References 31 publications

Circulating exosomal miR‑125a‑5p as a novel biomarker for cervical cancer

Circulating exosomal miR‑125a‑5p as a novel biomarker for cervical cancer

Decoding the role of extracellular vesicles in liver diseases

Circulating miR-122 levels in self-recovering hepatitis E patients

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