Serum and abomasal antibody response of sheep to infections with Haemonchus contortus

Charley-Poulain, Joelle; Luffau, G.; Pery, P.

doi:10.1016/0304-4017(84)90119-5

Cited by 28 publications

(8 citation statements)

References 18 publications

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“…In resistant sheep, the peak lgGl and IgA responses were 2 to 4 times greater than those detected in random-bred sheep. These results are broadly similar to the observations of others who reported significant increases in parasite-specific IgG and/or IgA antibodies in the serum and/or abomasal mucosa of immune sheep following challenge infection (Duncan et al 1978, Smith & Christie 1978, Charley-Poulain et al 1984. It is interesting to note, however, that the kinetics of faecal IgA and IgG1 in the present study were different from those observed in sera.…”

Section: Parasite Immunologysupporting

confidence: 92%

“…However, the kinetics of appearances of antibody isotypes induced following infection have not been identified. Increased concentrations of IgG in the serum and local IgA and/or IgG antibodies have been associated with resistance to gastrointestinal nematodes in outbred sheep (Smith & Christie 1978, Duncan, Smith & Dargie 1978, Charley-Poulain, Luffau & Perry 1984.…”

Section: Introductionmentioning

confidence: 99%

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Isotype‐specific antibody responses to Haemonchus contortus in genetically resistant sheep

Gill

Gray

Watson

et al. 1993

Parasite Immunology

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The kinetics of anti-Haemonchus antibody responses in serum and faecal extracts of pasture-reared, genetically resistant and random-bred sheep infected with Haemonchus contortus were examined using an isotype-specific ELISA. Anti-Haemonchus antibodies of IgA, IgG1, IgG2 and IgM isotypes were detected in serum and faecal extracts of both resistant and random-bred sheep after challenge infection. Serum IgG1 and IgA levels in resistant sheep were significantly higher than in random-bred sheep between 10 and 31 days after infection. However, there were no differences in IgG2 and IgM antibody responses between the two genotypes. Faecal antibody responses to H. contortus showed a clear genetic effect with resistant sheep exhibiting higher IgA levels throughout infection and higher IgG1 levels between 24 and 31 days after infection. Furthermore, serum IgG1 and IgA, and faecal IgA responses were negatively correlated with faecal egg counts in both genotypes on 17, 24 and 31 days after infection. Together, these results are taken to indicate that anti-parasite IgA and IgG1 antibodies may play an important role in genetically determined resistance of sheep to haemonchosis.

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Section: Parasite Immunologysupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Isotype‐specific antibody responses to Haemonchus contortus in genetically resistant sheep

Gill

Gray

Watson

et al. 1993

Parasite Immunology

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“…When Texel sheep sera were probed in the immunoassay with fraction A41, 17 of the 20 H. contortusinfected animals (lanes [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] clearly recognized the peptide (Fig. 2).…”

Section: Diagnostic Value Of A41 To Test H Contortus Infections In Smentioning

confidence: 99%

Serodiagnosis of Haemonchosis with a Somatic Antigen (Hc26) in Several Breeds of Sheep

et al. 2000

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Abstract. Sera from 53 sheep belonging to Castellano, Churro, Manchego, and Merino breeds were analyzed to test the diagnostic value of a 26-kD antigen from adult Haemonchus contortus at prepatency and early and late patency of experimental haemonchosis. Animals that received zero, 1, or 2 infections with the parasite were tested. In addition, sera from 20 experimentally infected and 10 noninfected Texel sheep were used to test the antigen. Sera from 37 infected animals at prepatency as well as at patency in primary and secondary infection were found positive with the 26-kD antigen. However, sera from 10 animals with the lowest worm burdens (second infection) did not recognize the antigen during early patency (day 28 postinfection). IgG1 was the only isotype implicated in antigen recognition because IgG2, IgA, and IgM, in the same sera, showed no reactivity with the peptide. Antigen specificity was confirmed because hyperimmune sera against infective larvae and adult stages of the most common gastrointestinal nematodes found in natural infections in sheep (Trichostrongylus colubriformis and Teladorsagia circumcincta) did not recognize this peptide. The antigen was recognized only by anti-adult H. contortus hyperimmune sera and appeared to be absent in the L3 parasite stage. In addition, the partial N-terminal amino acid sequence of the diagnostic peptide is reported.Sheep haemonchosis is a widespread parasitic disease in the temperate areas of the world. Diagnosis is based on coprological methods, which are time consuming and require specially trained personnel. A rapid and simple test with which a considerable number of samples could be processed at the same time would be advantageous. Circulating antigens could not be detected in infected animals, 22 and the assays developed to detect parasite antigens in the feces of infected animals, although occasionally able to detect surface antigens of the parasite, were not always related to the parasite burden. 10 In addition, methods used to detect specific DNA are restricted to patency and are expensive to use routinely. 6,23 The detection of specific serum antibodies against the parasite by ELISA has produced inconsistent results because of the complex nature of the antigen extract used 5 and limited value of the soluble extracts as an antigen source. 21 In addition, cross-antigenicity has been widely described among related and unrelated nematodes. 1,7,9,27,28 The use of less complex antigen mixtures, such as excretory/secretory products, has led to better results in ELISA tests, 24 but there is still some cross-reactivity between related nematodes, both in ELISA and in Western blotting. 25 From the Departamento de Patología Animal I, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain (Gó-mez-Muñoz, Domínguez, Fernández-Pérez, Méndez, de la Fuente, Alunda, Cuquerella), and the Servicio Territorial de Agricultura y Ganadería, Medina de Rioseco, 47800 Valladolid, Spain (Gómez-Iglesias).Received for publication July 31, 1998.A partially ch...

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“…IgM antibody concentrations were low in the first sampling and then rose and remained high in subsequence samplings until the end of the experiment. The decreased in IgA and IgG concentration in the serum compared to IgM is probably due to antibody-parasite antigen complexes (Charley-Poulain et al 1984). IgM concentrations in the serum were significantly higher compared to IgG and IgA, probably as it didn't play any significant role in the immune mechanism in goats against H. contortus infection.…”

Section: Immunoglobulin G a And Mmentioning

confidence: 88%

“…A previous study suggested that the IgG found in the abomasal mucous of sheep repeatedly challenged with H. contortus larvae was derived from blood whereas the IgA was locally produced (Smith 1977). In another study in sheep, the IgG, IgA and IgM antibody levels did not change materially after first infection by H. contortus and the levels rose slightly after administration of a challenge dose of infective larvae but this rise appeared later than the fall in faecal egg counts (Charley-Poulain et al 1984).…”

Section: Immunoglobulin G a And Mmentioning

confidence: 88%

Development of immunity in boer goats naturally infected with the nematode haemonchus contortus

Abdullah¹

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Haemonchus contortus (H. contortus) is a major gastrointestinal nematode that adversely affects goat production in tropical and subtropical countries leading to high economic losses due to lower growth rates, markedly reduced reproductive performance and mortality. Farmers can use different strategies including anthelmintic treatments, grazing management, vaccination, improved nutrition and selection of resistant animals in order to control or prevent their animals from infection by this nematode. This study was conducted to determine and describe the development of immunity in Boer goats kids based on their faecal egg count (FEC) and selected blood parameters in relation to specific antibody production against this nematode. Understanding the development of immunity could Many colleagues and organisations (listed below) have contributed to the success of this study, without their assistance this study will not be possible. My sincere appreciation goes to: • The Malaysia government through the Public Services Department of Malaysia for providing me the scholarship to further my study for a higher research degree. • Department of Veterinary Services, Malaysia for allowing me to go on study leave to undertake this degree.

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Serum and abomasal antibody response of sheep to infections with Haemonchus contortus

Cited by 28 publications

References 18 publications

Isotype‐specific antibody responses to Haemonchus contortus in genetically resistant sheep

Isotype‐specific antibody responses to Haemonchus contortus in genetically resistant sheep

Serodiagnosis of Haemonchosis with a Somatic Antigen (Hc26) in Several Breeds of Sheep

Development of immunity in boer goats naturally infected with the nematode haemonchus contortus

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