Serum Amyloid A Gene polymorphism and its association with lipid profile in Saudi Females with osteoporosis

Abstract

Background and Objective:Osteoporosis can be defined as a systemic skeletal disease characterized by low bone mass and micro architectural decline of bone tissue. Serum amyloid A (SAA) is a family of protein that increases up to 1,000-fold in blood during inflammation. In this study, we aimed to study the relationship between SAA1 gene polymorphism (rs12218) and lipid profile and osteoporosis.Methods:The study was performed on the female students of Taibah University in Al Medina, KSA during June 2014 to April 2015. According to BMD; osteoporosis group (138 students) and control group (128 students). All groups were subjected to; BMI, BMD, calcium, phosphorus, creatinine, lipid profile and SAA. Polymerase chain reaction and Real Time were done to determine the distribution of allele and genotype frequency of SAA (rs12218) C/T polymorphism.Results:This study shows that the TT genotype of rs12218 was more frequent in osteoporosis group than control group (P<0.001). Also, TT genotype and T allel was found to be associated with plasma total cholesterol, TG, LDLc, HDLc, Tscore, Zscore and SAA1 level in osteoporosis group (P=0.000, P=0.05, and P=0.000, P=0.000, P=0.01, P=0.02, P=0.000 respectively). The logistic regression analysis with and without lipid disorders in the osteoporosis group also show that the TT genotype of rs12218 still differed significantly between these two groups (P=0.001, OR=1.814, 95% CI: 0.719-4.577).Conclusion:The results of this study shows a significant association between TT genotype of rs12218 and both lipid level and osteoporosis in Saudi female population.

SAA1 gene polymorphisms in osteoporosis patients

“…Our results suggest that the SAA1 rs12218 polymorphism is not associated with the risk of osteoporosis in a Chinese Han population involving 300 cases and 350 controls. Additionally, the CC genotype could not significantly change the SAA1 expression; this result stands in contrast with that reported by Feng et al [10] study who found and increase, as well as to that reported by Abdu-Allah et al [11], who found a decrease. We failed to find any significant association between the C allele and other clinical parameters (age, BMI, TC, LDL, HDL, and T-score).…”

“…The disparities between previous studies and ours may be attributed to clinical heterogeneity, the use of different ethnic populations, and small sample sizes. Specifically, the C-allele frequencies of Asians were 0.189 [10] and 0.383 (the present study), which were significantly lower than that in Caucasians (0.734 [11]). This result may be reflected by the difference in the allele frequency of the SAA1 rs12218 polymorphism.…”

“…This SNP was also associated with the TC, HDL, LDL levels, and the BMD in osteoporosis patients [10]. Moreover, CC genotype carriers had a lower risk of osteoporosis than TT genotype carriers in a Saudi population including 138 osteoporosis patients and 128 controls [11]. The CC genotype and C allele were associated with TC, LDL, HDL, T-score, Z-score and lower SAA1 levels in osteoporosis patients [11].…”

“…Moreover, CC genotype carriers had a lower risk of osteoporosis than TT genotype carriers in a Saudi population including 138 osteoporosis patients and 128 controls [11]. The CC genotype and C allele were associated with TC, LDL, HDL, T-score, Z-score and lower SAA1 levels in osteoporosis patients [11]. Our results suggest that the SAA1 rs12218 polymorphism is not associated with the risk of osteoporosis in a Chinese Han population involving 300 cases and 350 controls.…”

“…SAA1 is located in chromosome 11p15.1 and has four exons. Although two studies have reported an association between the SAA1 rs12218 polymorphism and the risk of osteoporosis, their findings conflicted [10,11]. Therefore, this case–control study which enrolled 300 osteoporosis patients and 350 controls was conducted to evaluate the role of SAA1 gene polymorphisms in the risk of osteoporosis in a Chinese population.…”