Serum Amiloid-A'nın Sıçanlarda Formaldehit Kaynaklı Kupffer Hücre Apoptozundaki Rolü ve Astaksantin'in Bu Süreçteki Olası Koruyucu Etkileri

Abstract

The aim of this study is to investigate the alterations related to Serum amyloid-A in formaldehyde-induced apoptosis in Kupffer cells and to determine whether Astaxanthin has a protective effect against apoptosis. In this experiment, 32 rats were divided into 4 groups (n=8). The first group was named as control group, physiological saline was injected intraperitoneally to this group, and drinking water was given orally. In CH 2 O group, rats were injected with formaldehyde at a dose of 10 mg/kg daily intraperitoneally. The rats in CH 2 O+ATX16 and CH 2 O+ATX32 were injected with formaldehyde daily at a dose of 10 mg/kg intraperitoneally, and respectively 16 mg/kg and 32 mg/kg Astaxanthin were administered orally. Formaldehyde administration was caused by the highest and statistically significant Serum amyloid-A staining intensity (P<0.0125) and apoptotic index (P<0.05) in the CH 2 O group. Both doses of Astaxanthin administration reduced apoptosis in Kupffer cells but there were no significant differences in serum Serum amyloid-A levels between experimental groups (P>0.05). As a result, oral administration of Astaxanthin has been shown to reduce Serum Amyloid A, which increases due to exposure to formaldehyde, and possibly in this way, Kupffer cells successfully protect against formaldehyde-induced apoptosis. The subject should be examined more comprehensively.