Serum alkylresorcinols as biomarkers of dietary gluten exposure in coeliac disease

Abstract

Serum alkylresorcinol concentrations could be a useful marker for dietary gluten in coeliac disease.

Randomised controlled trial: effects of gluten‐free diet on symptoms and the gut microenvironment in irritable bowel syndrome

“…Although the use of serum alkylresorcinols as a biomarker for a gluten‐free diet remains to be validated in larger studies, several studies have shown its potential 7–9 . The findings in our sample that serum alkylresorcinols were lower after the gluten‐free intervention compared with the gluten‐containing intervention support this, and indicates good compliance to the interventions, which we consider as a strength.…”

“…Although the use of serum alkylresorcinols as a biomarker for a gluten-free diet remains to be validated in larger studies, several studies have shown its potential. [7][8][9] The findings in our sample that serum alkylresorcinols were lower after the gluten-free intervention compared with the gluten-containing intervention support this, and indicates good compliance to the interventions, which we consider as a strength. Another strength of this study is the adequate blinding of the participants and investigators, and confounding factors were minimised by using the randomised crossover design, 23 and the strict in-and exclusion criteria.…”

“…After both interventions, serum samples were taken and serum alkylresorcinols were analysed using liquid chromatography/mass‐spectrometry (LC–MS) (Food and Nutrition Science, Chalmers Technical University, Gothenburg, Sweden) for assessment of compliance to both gluten‐free and gluten‐containing interventions. Alkylresorcinols are long‐chain phenolic lipids present in gluten‐containing grains, and both epidemiological and intervention studies have shown the potential of alkylresorcinols as a biomarker for both gluten intake and a gluten‐free diet 7–9 …”

Community-Based Study of Celiac Disease Autoimmunity Progression in Adults

“…Serum AR concentrations, which can be used as a biomarker for gluten-containing diet in prior investigations, 22,23 were measured using the normal-phase liquid chromatographytandem mass spectrometry (LC-MS/MS), as described elsewhere 24 with some modifications for instrumentation. Briefly, 100 μL of serum was loaded on HybridSPE Plus phospholipid removal 96-well plates (Sigma-Aldrich, St Louis, MO, USA) and recovered with acetone.…”

Editorial: gluten‐free but not pain‐free in IBS—authors' reply

“…Finally, adherence to diet in intervention studies is always a matter of worry. Even if our measures of serum alkylresorcinols 9 as a biomarker for adhering to a GFD remains to be validated, numerous studies, including a gluten challenge study in healthy volunteers, 10 have shown its potential.…”