Serum alkaline phosphatase isoenzymes in laboratory beagle dogs detected by polyacrylamide-gel disk electrophoresis

Hatayama, Kazumi; Nishihara, Yoshito; Kimura, Sayaka; Goto, Ken; Nakamura, Daichi; Wakita, Atsushi

doi:10.2131/jts.36.653

Cited by 8 publications

(3 citation statements)

References 14 publications

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“…In the present study, we did not measure the levels of ALP and aspartate aminotransferase (AST), a liver enzyme, which are related to infections with B. canis and B. gibsoni [ 3 , 42 ]. There are three main ALP isoenzymes in canine serum [ 43 ] and the elevation of canine ALP is also associated with hepatobiliary, hepatic, and bone diseases [ 44 - 46 ]. In azotemic dogs infected with B. canis , the AST/ALT ratio decreased [ 47 ], and this ratio was not significantly different from those observed in B. vogeli infections [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

Clinicopathological and molecular profiles of Babesia vogeli infection and Ehrlichia canis coinfection

Rawangchue

Sungpradit

2020

Vet World

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Background and Aim: Canine babesiosis, a tick-borne parasitic disease, is caused by the hemoprotozoa, Babesia vogeli, and Babesia gibsoni. Infection with these parasites, which is endemic globally, leads to life-threatening immunosuppression in dogs. The merozoites invade the red blood cells (RBCs) of infected dogs. Ehrlichia canis, an intracellular bacterium that infects monocytes, is transmitted by the same tick species (Rhipicephalus sanguineus) during blood consumption and coinfection with B. vogeli and E. canis has been reported. Although the hematology and biochemistry of canine babesiosis have been studied, more studies are needed to develop a better understanding of the hematobiochemical and molecular profiles associated with cases of single infection and coinfection of canine babesiosis in Thailand. This study aimed to investigate the hematological, biochemical, and molecular profiles of B. vogeli infection and E. canis coinfection. Materials and Methods: The study included 33 B. vogeli–positive blood samples and 11 E. canis–coinfected blood samples. To exclude coinfection with Hepatozoon canis and Anaplasma platys, only dogs with B. vogeli infection and B. vogeli–E. canis coinfection were included in the study. A multiplex polymerase chain reaction (PCR) assay was conducted to detect B. vogeli, E. canis, and H. canis, and a conventional PCR assay was conducted for the detection of A. platys. Besides, the PCR assay and sequencing, comprehensive data analysis was conducted, including a microscopic blood parasite examination and hematological and biochemical data analysis. Results: The comparison of the hematobiochemical data between the B. vogeli–positive and E. canis coinfection groups identified that there were statistically significant differences in the RBC parameters, including RBC count, hemoglobin concentration, hematocrit, and RBC distribution width (p=0.001). Neither B. vogeli infection nor coinfection with E. canis was associated with the sex, breed, recorded clinical signs, geographic origin of the dog and also B. vogeli 18S rRNA gene sequencing results. Conclusion: Coinfection with E. canis increased the severity of babesiosis. The pathogenic mechanisms underlying this infection, such as destruction of RBCs, require further investigation. This study may enhance diagnosis, treatment, and prevention of canine babesiosis.

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Section: Discussionmentioning

confidence: 99%

Clinicopathological and molecular profiles of Babesia vogeli infection and Ehrlichia canis coinfection

Rawangchue

Sungpradit

2020

Vet World

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“…Irregular familiar feeding habits are considered the main cause of the dog higher phosphatase alkaline levels because of its role in the digestion and absorption of fats in the intestinal tract [61] and cannot be related to the immunization. The risk of an inflammatory response was also fully discarded by the clinical follow-up of all animals and the normality of the frequent plasma and CSF analyses.…”

Section: Discussionmentioning

confidence: 99%

Rapid Improvement of Canine Cognitive Dysfunction with Immunotherapy designed for Alzheimer's Disease

Bosch¹,

Bayon²,

Rodrı́guez³

et al. 2013

CAR

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ABSTRACT:Immunotherapy against amyloid-beta (Aβ) may improve rodent cognitive function by reducing amyloid neuropathology and is being validated in clinical trials with positive preliminary results. However, for a complete understanding of the direct and long-term immunization responses in the aged patient, and also to avoid significant side effects, several key aspects remain to be clarified. Thus, to investigate brain Aβ clearance and Th2 responses in the elderly, and the reverse inflammatory events not found in the immunized rodent, better Alzheimer's disease (AD) models are required. In the aged familiar canine with a cognitive dysfunction syndrome (CDS) we describe the rapid effectiveness and the full safety profile of a new active vaccine candidate for human AD prevention and treatment. In these aged animals, besides a weak immune system, the antibody response activated a coordinated central and peripheral Aβ clearance, that rapidly improved their cognitive function in absence of any side effects. Our results also confirm the interest to use familiar dogs to develop innovative and reliable therapies for AD.

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“…In dogs at rest, the activity of biochemical indicators of bone remodeling in serum, the differences with regard to age, sex, size, breed, the influence of hormones and daily variability have been investigated [3,[6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

The Effect of a Moderate Exercise Program on Serum Markers of Bone Metabolism in Dogs

Vrbanac

Bottegaro

Škrlin

et al. 2020

Animals

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The beneficial effect of physical activity on the musculoskeletal health in dogs is well recognized, but the level of intensity, duration, and frequency of exercise is not fully described. Measurement of serum markers of bone metabolism (bone alkaline phosphatase and osteocalcin as bone formation markers and C-terminal telopeptide as bone resorption marker) during four months of organized moderate-intensity physical training in Labrador retriever and Golden retriever dogs aged between 11.7–24.4 months, showed variations of bone metabolism. Dogs were included in treadmill running sessions for 25 min, three times per week. Blood samples were taken at the beginning of the program (baseline), after two months (mid-term) and at the end of the study after four months. The values of bone alkaline phosphatase and osteocalcin significantly decreased following two months of exercise program. Bone alkaline phosphatase increased by the end of four-month training cycle, but did not reach baseline value. Osteocalcin levels continued to decrease towards the end of the study. C-terminal telopeptide concentrations did not significantly change throughout the study duration. The results of this study show that aerobic exercise of moderate-intensity caused an initial decrease in bone formation followed by an increase of bone alkaline phosphatase and a further decrease of osteocalcin concentration. The response of two formation markers can be explained by the different stage of osteoblast activity that they express. In summary, moderate exercise resulted in no change in bone resorption, and a mild bone formation in young developing dogs.

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Serum alkaline phosphatase isoenzymes in laboratory beagle dogs detected by polyacrylamide-gel disk electrophoresis

Cited by 8 publications

References 14 publications

Clinicopathological and molecular profiles of Babesia vogeli infection and Ehrlichia canis coinfection

Clinicopathological and molecular profiles of Babesia vogeli infection and Ehrlichia canis coinfection

Rapid Improvement of Canine Cognitive Dysfunction with Immunotherapy designed for Alzheimer's Disease

The Effect of a Moderate Exercise Program on Serum Markers of Bone Metabolism in Dogs

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