Serum alkaline phosphatase isoenzymes in SD rats detected by polyacrylamide-gel disk electrophoresis

Hatayama, Kazumi; Ichikawa, Yuko; Nishihara, Yoshito; Goto, Ken; Nakamura, Daichi; Wakita, Atsushi; Kobayashi, Jyunichi

doi:10.3109/15376516.2011.654005

Cited by 8 publications

(5 citation statements)

References 18 publications

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“…ALP and OC are two typical indicators reflecting osteoblast activity, and their levels are related to osteoblast-regulated bone formation ( Seibel, 2006 ). Previous studies showed that the levels of serum ALP and OC were significantly increased after bone injury, reflecting increased osteoblast activity ( Hatayama et al, 2012 ; Kubo et al, 2012 ). TRAP is a common index used to evaluate the activity of osteoclasts and macrophages during bone remodeling.…”

Section: Discussionmentioning

confidence: 99%

Serum bone remodeling parameters and transcriptome profiling reveal abnormal bone metabolism associated with keel bone fractures in laying hens

Wei¹,

Bi²,

Wang³

et al. 2023

Poultry Science

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Section: Discussionmentioning

confidence: 99%

Serum bone remodeling parameters and transcriptome profiling reveal abnormal bone metabolism associated with keel bone fractures in laying hens

Wei¹,

Bi²,

Wang³

et al. 2023

Poultry Science

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“…There are three isoforms of ALP detected in rodents’ blood. In young mature Sprague Dawley rats, primarily an osteal and small intestine isoform is detected [ 55 ]; in the plasma of outbred mice, the main isoenzyme ALP is of osteal origin, but liver isoenzyme is also detected [ 56 ]. Still, the total level of ALP in rodents’ blood increases in the modeled conditions of cholestasis and directly correlates with the increase in the concentration of bile acids [ 57 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative Study of the Aftereffect of CO2 Inhalation or Tiletamine–Zolazepam–Xylazine Anesthesia on Laboratory Outbred Rats and Mice

et al. 2022

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CO2 inhalation is currently the most common method of euthanasia for laboratory rats and mice, and it is often used for further terminal blood sampling for clinical biochemical assays. Lately, this method has been criticized due to animal welfare issues associated with some processes that develop after CO2 inhalation. The stress reaction and the value of the clinical laboratory parameters significantly depend on the used anesthetics, method, and the site of blood sampling. Especially in small rodents, an acute terminal state followed by a cascade of metabolic reactions that can affect the studied biochemical profile may develop and cause unnecessary suffering of animals. The aim of this study was to compare the stability of biochemical parameters of outbred Sprague Dawley rats and CD-1 mice serum collected after CO2 inhalation or the intramuscular injection of tiletamine–zolazepam–xylazine (TZX). The serum content of total protein and albumin, cholesterol, triglycerides, aspartate aminotransferase (AST), alanine aminotr ansferase (ALT), alkaline phosphatase (ALP), total bilirubin, and creatinine was decreased by the injection of TZX in comparison with CO2 inhalation. In addition, the levels of calcium, phosphates, chlorides and potassium were lowered by TZX vs. CO2 administration, while the level of sodium increased. Finally, the level of the majority of serum clinical biochemical parameters in rats and mice tend to be overestimated after CO2 inhalation, which may lead to masking the possible effect of anti-inflammatory drugs in animal tests. Injection anesthesia for small rodents with TZX is a more feasible method for terminal blood sampling, which also reduces the suffering of animals.

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“…is derived mostly from bone and intestine. It is widely known that the amount of intestine-derived isoenzymes is high under satiation and low when fasting (Hatayama et al, 2012). Thus, the total ALP in rats is greatly affected by food intake.…”

Section: Discussionmentioning

confidence: 99%

Safety evaluation of a newly-developed dietary fiber: resistant glucan mixture

et al. 2016

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Resistant glucan mixture (RGM), a water-soluble dietary fiber produced by the random polymerization of glucose with activated carbon as a catalyst at a high temperature, has been recently developed by our group. There has been little physiological and safety research into RGM and therefore we now present our research into its safety. A reverse mutation assay indicated that RGM is not mutagenic either with or without metabolic activation. We conducted a 90-day subchronic oral toxicity study in rats. Male and female rats fed either a 3% or 5% w/w RGM diet had no muddy or watery stools, and there was no RGM-related death in any group. Although some parameters in the 3% and 5% w/w groups were significantly different from those in the control group, these changes were not due to any toxicity from RGM. The results indicated that the No Observed Adverse Effect Level (NOAEL) of RGM was 3.3 and 3.9 g/kg body weight (BW) per day in male and female rats, respectively. We then studied the gastrointestinal effects of RGM in healthy adult humans. Gastrointestinal symptoms, such as gurgling sounds, flatus and tenesmus, were mild and transient. In men and women, the maximum no-effect dose for diarrhea was more than 0.9 g RGM /kg BW. The results of our current safety assessment studies suggest that RGM is safe for human consumption.

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Serum alkaline phosphatase isoenzymes in SD rats detected by polyacrylamide-gel disk electrophoresis

Cited by 8 publications

References 18 publications

Serum bone remodeling parameters and transcriptome profiling reveal abnormal bone metabolism associated with keel bone fractures in laying hens

Serum bone remodeling parameters and transcriptome profiling reveal abnormal bone metabolism associated with keel bone fractures in laying hens

Comparative Study of the Aftereffect of CO2 Inhalation or Tiletamine–Zolazepam–Xylazine Anesthesia on Laboratory Outbred Rats and Mice

Safety evaluation of a newly-developed dietary fiber: resistant glucan mixture

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