Serum albumin in 2D: A Langmuir monolayer approach

Crawford, Nicholas F.; Leblanc, Roger M.

doi:10.1016/j.cis.2013.10.021

Cited by 23 publications

(20 citation statements)

References 108 publications

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“…Cellular monolayer assays are commonly used as research tools for drug screening, and are widely applied in molecular biology for the identification of different molecular pathways, among other utilizations 8 , 9 . Drug screening of compound libraries for various activities, such as anti-cancer activity, relies mainly on cytotoxicity assays, using established cancer cell lines grown in 2-dimentional (2D) cultures that exhibit rapid growth kinetics.…”

Section: Introductionmentioning

confidence: 99%

Tumor cells and their crosstalk with endothelial cells in 3D spheroids

Shoval

Karsch-Bluman

Brill-Karniely

et al. 2017

Sci Rep

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Recapitulating the tumor microenvironment is a central challenge in the development of experimental model for cancer. To provide a reliable tool for drug development and for personalized cancer therapy, it is critical to maintain key features that exist in the original tumor. Along with this effort, 3-dimentional (3D) cellular models are being extensively studied. Spheroids are self-assembled cell aggregates that possess many important components of the physiological spatial growth and cell-cell interactions. In this study we aimed to investigate the interconnection between tumor and endothelial cells (EC) in hybrid spheroids containing either tumor cell (TC) lines or patient derived cancer cells. Preparation protocols of hybrid spheroids were optimized and their morphology and tissue-like features were analyzed. Our finding show that capillary-like structures are formed upon assembly and growth of TC:EC spheroids and that spheroids’ shape and surface texture may be an indication of spatial invasiveness of cells in the extra-cellular matrix (ECM). Establishing a model of hybrid tumor/stroma spheroids has a crucial importance in the experimental approach for personalized medicine, and may offer a reliable and low-cost method for the goal of predicting drug effects.

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Section: Introductionmentioning

confidence: 99%

Tumor cells and their crosstalk with endothelial cells in 3D spheroids

Shoval

Karsch-Bluman

Brill-Karniely

et al. 2017

Sci Rep

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“…Pure DOTAP monolayer and mixed DOTAP-BSA monolayer were transferred onto freshly cleaved micas at the surface pressures of 25 mN/m (after 30 minutes) and 15 mN/m (after two hours), respectively, with a vertical pulling method 36 . The surface morphology feature of deposited monolayer was directly visualized by using an SPM-9500-J3 AFM (Shimadzu Corporation, Japan) in the contact mode.…”

Section: Methodsmentioning

confidence: 99%

The interaction between BSA and DOTAP at the air-buffer interface

Hao

Zhang

et al. 2018

Sci Rep

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In this article, the interaction between bovine serum albumin (BSA) and the cationic 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) at the air-buffer interface was investigated at different subphase’s pH values (pH = 3, 5 and 10). Surface pressure measurements (π − A) and penetration kinetics process (π − t) were carried out to reveal the interaction mechanism and the dynamical behavior. The data showed that π − A isotherms moved towards larger mean molecular area when the concentration of BSA ([BSA]) increased, the amount of BSA adsorbed onto DOTAP monolayer reached a threshold value at a [BSA] of 5 × 10−8 M, and BSA desorbed from the lipid monolayer as time goes by. The results revealed that the association of BSA with DOTAP at the air-buffer interface was affected by the subphase’s pH value. When pH = 10, the interaction mechanism between them was a combination of hydrophobic interaction and electrostatic attraction, so BSA molecules could be well separated and purified from complex mixtures. AFM images demonstrated that pH value and [BSA] could affect the morphology feature of DOTAP monolayer and the adsorption and desorption processes of BSA. So the study provides an important experimental basis and theoretical support for learning the interaction mechanism among biomolecules in separation and purification of biomolecules and biosensor.

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“…Low molecular weight amphiphiles, including (phospho)lipids and organic surfactants, are used initially for the formation of Langmuir layers due to their appropriate hydrophilichydrophobic balance, enabling the molecular orientation at the air-water interface. In the middle of the twentieth century, the idea of Langmuir monolayers was extended towards the description of the film formation of non-classical amphiphilic materials, such as (poly)peptides [44,45], proteins, [46] polysaccharides [47], nanoparticles [48], dyes [49,50], fullerenes [51][52][53] and (biodegradable) polymers [54,55].…”

Section: General Principlesmentioning

confidence: 99%

Evaluating polymeric biomaterial–environment interfaces by Langmuir monolayer techniques

Schöne

Roch

Schulz

et al. 2017

J. R. Soc. Interface.

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Polymeric biomaterials are of specific relevance in medical and pharmaceutical applications due to their wide range of tailorable properties and functionalities. The knowledge about interactions of biomaterials with their biological environment is of crucial importance for developing highly sophisticated medical devices. To achieve optimal performance, a description at the molecular level is required to gain better understanding about the surface of synthetic materials for tailoring their properties. This is still challenging and requires the comprehensive characterization of morphological structures, polymer chain arrangements and degradation behaviour. The review discusses selected aspects for evaluating polymeric biomaterial-environment interfaces by Langmuir monolayer methods as powerful techniques for studying interfacial properties, such as morphological and degradation processes. The combination of spectroscopic, microscopic and scattering methods with the Langmuir techniques adapted to polymers can substantially improve the understanding of their behaviour.

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Serum albumin in 2D: A Langmuir monolayer approach

Cited by 23 publications

References 108 publications

Tumor cells and their crosstalk with endothelial cells in 3D spheroids

Tumor cells and their crosstalk with endothelial cells in 3D spheroids

The interaction between BSA and DOTAP at the air-buffer interface

Evaluating polymeric biomaterial–environment interfaces by Langmuir monolayer techniques

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