Serum Agar Cultures of Blood and Bone Marrow Cells

Woodliff, H. J.

doi:10.1159/000206546

Cited by 4 publications

(2 citation statements)

References 4 publications

(7 reference statements)

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“…The use of in vitro culture is an obvious way to investigate this problem, but until the early 1 960s the majority of research workers found the culture of normal and abnormal haemopoietic cells to be very difficult (Woodliff, 1964). In a variety of systems (Woodliff, 1964;Woodliff, 1958;Woodliff, 1961;Osgood and Brooke, 1958;Lewis and Lewis, 1911;Carrel and Burrows, 1911) cells only survived a few days and showed little evidence of multiplication or differentiation.…”

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confidence: 99%

Diagnostic and prognostic significance of peripheral blood cultural characteristics in adult acute leukaemia

Balkwill¹,

Oliver²

1976

Br J Cancer

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Summary.-A simple liquid culture technique has been used to study peripheral blood from patients with acute myelogenous leukaemia. Evidence is presented that cells from morphologically identical types of leukaemia have differing capacity for " differentiation " from free floating blast cells into plastic-adherent, phagocytic, trypsin-resistant macrophage-like cells with Fc and C 3 receptors. Preliminary analysis suggests that patients whose cells have the greatest capacity for " differentiation " have a better chance of achieving complete remission.

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confidence: 99%

Diagnostic and prognostic significance of peripheral blood cultural characteristics in adult acute leukaemia

Balkwill¹,

Oliver²

1976

Br J Cancer

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“…Numerous studies of bone marrow cultured in nutrient media have been made since Carrel and Burrows [2] and L ew is and Lewis [7], W oodliff [19] has cultured bone marrow particles in agar in the pres ence of normal human serum and noted the following sequence of events: Fig. 4.…”

Section: Discussionmentioning

confidence: 99%

An <i>in vitro</i> Demonstration of the Ability of Human Bone Marrow Stromal Elements to Sustain Granulocytopoiesis

Altman¹

1977

Acta Haematol

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The ability of human bone marrow particles to produce a microenvironment conducive to granulocytopoiesis was tested by culturing them in vitro without an exogenous source of colony stimulating activity (CSA). Granulocytopoiesis in this system was confirmed by the following observations: (1) presence of mitotic figures in promyelocytes and myelocytes; (2) early disappearance of mature granulocytes, followed by their reemergence after 4 days in culture, and (3) presence of immature granulocytes even after 10–14 days in culture. Although no exogenous source of CSA was added to the culture plates, a probable endogenous source was the dense accumulation of stromal elements in the core of particles; these cells may generate sufficiently high local levels of CSA to stimulate and nurture granulocyte proliferation and maturation

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Viability assays for preserved cells, tissues, and organs

Pegg

1989

Cryobiology

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Serum Agar Cultures of Blood and Bone Marrow Cells

Cited by 4 publications

References 4 publications

Diagnostic and prognostic significance of peripheral blood cultural characteristics in adult acute leukaemia

Diagnostic and prognostic significance of peripheral blood cultural characteristics in adult acute leukaemia

An <i>in vitro</i> Demonstration of the Ability of Human Bone Marrow Stromal Elements to Sustain Granulocytopoiesis

Viability assays for preserved cells, tissues, and organs

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