Serum Aberrant Expression of miR-24-3P and Its Diagnostic Value in Alzheimer’s Disease

Abstract

Aim: This study aimed to evaluate the effect of miR-24-3p in Alzheimer’s disease (AD). Materials & methods: A total of 198 participants were recruited in this study, including 104 AD patients and 94 healthy controls. Expression of miR-24-3p was detected using quantitative real-time PCR. Receiver-operating characteristic curve was used to assess the diagnostic value of miR-24-3p. In vitro AD model was established to evaluate the effect of miR-24-3p. The downstream target was detected by luciferase reporter gene assay. Results: Expression of miR-24-3p showed 1.6-fold increase in AD group compared with healthy controls, and a negative correlation of miR-24-3p with mini-mental state examination score was obtained. Receiver-operating characteristic curve showed satisfactory diagnostic accuracy. Downregulation of miR-24-3p promoted cell proliferation and inhibited cell apoptosis. KLF8 is a target gene of miR-24-3p. Conclusion: MiR-24-3p has a certain value in the diagnosis of AD and may be a potential biomarker.

The Relationship between the Aberrant Long Non-Coding RNA-Mediated Competitive Endogenous RNA Network and Alzheimer’s Disease Pathogenesis

“…Although Xist is closely related to sex, the influence of sex factors on the transcription of Xist in AD pathology is still on the exploration road. ENSMUSG00000098912, a significantly downregulated lncRNA, had the highest Degree value (compared to known lncRNAs) in the downregulated lncRNA-mediated ceRNA network and interacted with a wide range of miRNAs, including miR-214-3p, miR-24-3p, miR-743b-3p, etc., and shows multiple correlations with pathologies of autophagy and apoptosis in AD [ 32 , 33 ]. The GO and KEGG analysis results provide a basic understanding of the biological characteristics of the novel lncRNAs, suggesting they act as ceRNAs through the neurotrophin signaling pathway, axon guidance, and hippo signaling pathway.…”

miRNA Expression Is Increased in Serum from Patients with Semantic Variant Primary Progressive Aphasia

“…Of note, our data show a deregulation, not only of miR-223-3p that is widely researched in AD [ 18 ], but also of miR-125b-5p, miR-24-3p and miR-21-5p. These molecules that are downregulated in lvPPA patients (although not reaching statistical significance) are already associated with AD suggesting their possible role in the amyloid metabolism defect [ 19 , 20 , 21 , 22 ].…”

The Potential Role of miRNA-Regulated Autophagy in Alzheimer’s Disease

“…In human serum, the up-regulated miR-24-3 and down-regulated miR-193a-3p may promote neuronal apoptosis [ 48 , 49 ]; miR-4422-5p can target gamma-secretase-activating protein (GSAP) and beta-site APP-cleaving enzyme-1 (BACE1) to exacerbate Aβ formation [ 50 , 51 ]; the down-regulated miR-148a-3p can trigger the neurotoxicity of Aβ to the nervous system [ 52 ]; the reduced levels of miR-222, miR-223, miR-29c-3p, and miR-19b-3p in blood samples of AD patients, and the increased level of miR-501-3p, have the potential to predict the occurrence and progression of AD [ 53 , 54 , 55 , 56 ]. At the same time, miR-331-3p may have a potential neuroprotective effect in AD by inhibiting neuroinflammation [ 57 ], the elevated miR-1273g-3p can aggravate mitochondrial damage and Aβ production [ 58 ], and miR-545-3p, miR-222, miR-125b, miR-455-3p, and miR-34a-5p have been confirmed to have the potential for the diagnosis and treatment of AD [ 59 ].…”