Serum 8-Hydroxy-2′-Deoxyguanosine Level as a Potential Biomarker of Oxidative DNA Damage Induced by Ionizing Radiation in Human Peripheral Blood

Abstract

In this study, the effect of ionizing radiation on 8-hydroxy-2′-deoxyguanosine (8-OHdG) in human peripheral blood was investigated. Blood samples were collected from 230 radiation workers and 8 patients who underwent radiotherapy for population study. Blood samples from 2 healthy individuals were irradiated with different X-ray doses for in vitro experiment, and levels of 8-OHdG in serum and cell culture supernatants were assessed by enzyme-linked immunosorbent assay. Observations demonstrated the positive relationships between serum 8-OHdG level and radiation dose and working period were observed, and serum 8-OHdG levels were higher among interventional radiation workers than among other hospital radiation workers. In addition, 8-OHdG yields in supernatants increased, peaked at 3 Gy of radiation dose, and then decreased with further increases in radiation; the dose–response curve obtained fitted a polynomial function. By contrast, a similar trend was not found in radiotherapy patients. The present study suggests that 8-OHdG may be a useful biomarker reflecting oxidative damage among workers occupationally exposed to low-dose radiation.

8-Hydroxy-2’-Deoxyguanosine and 8-Nitroguanine Production and Detection in Blood Serum of Breast Cancer Patients in Response to Postoperative Complementary External Ionizing Irradiation of Normal Tissues

“…Gao and his colleagues 23 demonstrated that serum 8-OHdG levels were significantly elevated in interventional radiologists compared with other categories of radiation workers, highlighting that the extend of oxidative DNA damage is proportional to the radiation dose. In other words, increasing radiation doses result in a more severe oxidative DNA damage, thereby indicating a distinct dose-response relationship.…”

“…By contrast, there is another point of view regarding prolonged irradiation which primarily concerns cancer patients receiving radiotherapy; 8-OHdG levels not only can be decreased but also can be found lower than those of healthy subjects due to DNA repair mechanisms. 22,23 Gao and his colleagues 23 demonstrated that serum 8-OHdG levels were significantly elevated in interventional radiologists compared with other categories of radiation workers, highlighting that the extend of oxidative DNA damage is proportional to the radiation dose. In other words, increasing radiation doses result in a more severe oxidative DNA damage, thereby indicating a distinct dose-response relationship.…”

“…By contrast, there is another point of view regarding prolonged irradiation which primarily concerns cancer patients receiving radiotherapy; 8-OHdG levels not only can be decreased but also can be found lower than those of healthy subjects due to DNA repair mechanisms. 22,23…”

“…Further studies implied the reduction of urinary 8-OHdG levels upon radiotherapy as well as absence of a linear correlation of 8-OHdG with the accumulated radiation dose. 23 There are different ways for explaining the declined 8-OHdG levels upon radiation exposure. First of all, DNA repair systems negate DNA damage induced by radiation and consequently such lesions cannot be accumulated.…”

OGG1 DNA Repair Gene Polymorphism As a Biomarker of Oxidative and Genotoxic DNA Damage

“…An oxidized form of guanosine, 8-OHdG, is a biomarker of oxidative DNA damage and is involved in the pathology of many diseases such as cancer, atherosclerosis, and diabetes [ 3 ] . Base excision DNA repair pathway recognizes 8-OHdG as a threatening lesion, which results in its excretion in human urine without further metabolism [ 4 ] .…”