Serum 25-hydroxyvitamin D levels in relation to lipids and clinical outcomes in pregnant women with gestational diabetes mellitus: an observational cohort study

Chen, Wenqing; Li, Yuan; Gao, Bo; Li, Jie; Zheng, Mingming; Chen, Xiaotian

doi:10.1136/bmjopen-2020-039905

Cited by 6 publications

(3 citation statements)

References 25 publications

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“…Recently, several studies investigated the upstream regulatory mechanism for the expression of CBS and CSE, and the production of H 2 S, and the results demonstrated that high fat (46,47), high salt (48), hypoxia (49) and oxidative stress (50) inhibited the expression of CBS and CSE, and the production of H 2 S. Conversely, vitamin D supplementation increased CSE expression and the production of H 2 S (51). Other clinical studies have reported that high-fat and high-salt diet, vitamin D deficiency during pregnancy (52)(53)(54), hypoxia and oxidative stress in the placenta (55,56) are associated with the pathogenesis of GDM. Taken together, these results suggest that high-fat and high-salt diet, vitamin D, hypoxia and oxidative stress may be upstream regulatory factors for the expression of CBS and CSE, and the production of H 2 S in GDM.…”

Section: Discussionmentioning

confidence: 90%

NLRP3 inflammasome activation in gestational diabetes mellitus placentas is associated with hydrogen sulfide synthetase deficiency

Tan

et al. 2021

Exp Ther Med

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The placenta may play a key role in the activation of inflammation and initiation of insulin resistance (IR) during gestational diabetes mellitus (GDM) pathogenesis. Interleukin (IL)-1β and IL-18, regulated by NLR family pyrin domain containing-3 (NLRP3) inflammasome, are important inflammatory cytokines in the initiation of maternal IR during GDM. However, the mechanism responsible for the regulatory of NLRP3 inflammasome in placenta remains unknown. Hydrogen sulfide (H 2 S) exerts anti-inflammatory function partially via suppressing the activation of the NLPR3 inflammasome. The present study aimed to investigate the role of NLRP3 inflammasome, H 2 S synthetase cystathionine-γ-lyase (CSE) and cystathionine-β-synthetase (CBS) in placenta in the pathogenesis of GDM. Clinical placenta samples were collected from pregnant women with GDM (n=16) and healthy pregnant women at term (n=16). Western blot analysis was performed to detect the protein expression levels of NLRP3, cleaved caspase-1, CBS and CSE in the placenta samples. Pearson's correlation analysis was performed to assess the correlation between NLRP3 inflammasome and H 2 S synthetase. Human placental cells were cultured and treated with different concentrations of NaHS (0, 10, 25 and 50 nmol/l) or L-cysteine (0, 0.25, 0.50 and 1.00 mmol/l). In addition, western blot analysis was performed to detect the protein expression levels of NLRP3 and cleaved caspase-1, while ELISA was performed to measure the production of IL-1β and IL-18 in the culture media. The results demonstrated that the expression levels of NLRP3 and cleaved caspase-1 increased, while the expression levels of CBS and CSE decreased in the placenta samples. In addition, the expression levels of NLRP3 and cleaved caspase-1 were inversely correlated with the expression levels of CBS and CSE. Notably, NaHS and L-cysteine significantly suppressed the expression levels of NLRP3 and cleaved caspase-1, and the production of IL-1 and IL-18 in human placental cells. Taken together, the results of the present study suggest that H 2 S synthetase deficiency in placenta may contribute to excessive activation of NLRP3 inflammasome in GDM.

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Section: Discussionmentioning

confidence: 90%

NLRP3 inflammasome activation in gestational diabetes mellitus placentas is associated with hydrogen sulfide synthetase deficiency

Tan

et al. 2021

Exp Ther Med

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“…Vitamin D is well known in regulating calcium and phosphorus metabolism. It also plays an important role in glucose and lipid metabolism, immune response and so on 1 . Vitamin D deficiency during pregnancy is a global public health problem 2 .…”

Section: Introductionmentioning

confidence: 99%

“…It also plays an important role in glucose and lipid metabolism, immune response and so on. 1 Vitamin D deficiency during pregnancy is a global public health problem. 2 25(OH) D is commonly used to reflect the state of vitamin D in human.…”

Section: Introductionmentioning

confidence: 99%

The association between vitamin D levels in the second trimester of pregnancy and gestational diabetes mellitus

Wang

Jiao

et al. 2022

J of Obstet and Gynaecol

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Background and aims: 25-hydroxyvitamin D (25(OH)D) affects glucose metabolism by increasing insulin secretion and insulin receptor expression. However, whether 25(OH)D deficiency will increase the risk of gestational diabetes mellitus (GDM) has not been clearly reported. The purpose of this study is to assess the relationship between vitamin D levels in the second trimester of pregnancy and the risk of GDM. Methods: According to the inclusion and exclusion criteria, 247 pregnant women came to the fourth hospital of Shijiazhuang (The affiliated obstetrics and gynecology hospital of Hebei Medical University) for obstetrics were investigated during the period of January 1, 2019 to December 31, 2020. The levels of 25(OH)D in the second trimester (16-20 weeks) and oral 75 g glucose tolerance test (OGTT) at 24-28 weeks of pregnancy were reviewed. The sociodemographic data were collected from questionnaire. Multivariate logistic regression was used to analyze the relationship between vitamin D levels and GDM. Results: The incidence of GDM in the observation group (25(OH)D ≤ 26 ng/ml) was higher than that in the control group (25(OH)D > 26 ng/ml) (p = 0.039). Compared with control group, the observation group had significantly higher level of fasting plasma glucose (FPG) (4.7 [4.5-5.0] mmol/L vs. 4.6 [4.4-4.8] mmol/L, p = 0.012). In the whole study, the level of 25(OH)D was negatively correlated with FPG (r = À 0.164， p = 0.010). After adjusting for age, pre-pregnancy BMI, parity and adverse pregnancy history, compared with the observation group (25 (OH) D ≤ 26 ng/ml), the risk of developing GDM decreased by 50.9% in control group (25(OH)D > 26 ng/ml) (odds ratio [OR] = 0.491, 95% confidence interval [CI] = 0.243-0.989, p = 0.047). Conclusion: Adequate vitamin D levels during the second trimester of pregnancy may reduce the risk of GDM.

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Mediating Effect of Insulin-Like Growth Factor-I Underlying the Link Between Vitamin D and Gestational Diabetes Mellitus

Cui,

Li,

Yang

et al. 2024

Reprod. Sci.

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Serum 25-hydroxyvitamin D levels in relation to lipids and clinical outcomes in pregnant women with gestational diabetes mellitus: an observational cohort study

Cited by 6 publications

References 25 publications

NLRP3 inflammasome activation in gestational diabetes mellitus placentas is associated with hydrogen sulfide synthetase deficiency

NLRP3 inflammasome activation in gestational diabetes mellitus placentas is associated with hydrogen sulfide synthetase deficiency

The association between vitamin D levels in the second trimester of pregnancy and gestational diabetes mellitus

Mediating Effect of Insulin-Like Growth Factor-I Underlying the Link Between Vitamin D and Gestational Diabetes Mellitus

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