Serum 14-3-3η protein is associated with clinical and serologic features of Sjögren’s syndrome in patients with systemic lupus erythematosus: a cross-sectional analysis

Hammam, Nevin; Gamal, Nada; Elzohri, Mona H.; Elsonbaty, Amira; Rashed, Ahmed M.; Eldaly, Zeiad H; Tarik, Dalia; Gheita, Tamer A.

doi:10.1007/s10067-020-05033-3

Cited by 6 publications

(6 citation statements)

References 27 publications

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“…The relationships of the 14-3-3 protein isoforms with neoplastic diseases, RA, and secondary PSS are well known. 25,[29][30][31][32][33][34][35][36][37][38][39][40][41][43][44][45][46] In this study, we investigated the utility of the 14-3-3η protein as a diagnostic marker, disease activity indicator, and lymphoma predictor in PSS. Our results demonstrated that plasma 14-3-3η protein levels were higher in PSS than healthy controls (P < 0.0001), and the cut-off value of 1.741 for the 14-3-3η protein had a sensitivity of 51.9% and a specificity of 84.2% in the diagnosis of PSS (AUC [95% Cl] = 0.758 [0.669-0.848], P < 0.0001).…”

Section: Discussionmentioning

confidence: 99%

“…23,42 The 14-3-3η proteins are also associated with RA, joint erosion in RA, and secondary Sjogren's syndrome (SSS) due to systemic lupus erythematosus (SLE). 25,[43][44][45][46] It is essential to predict the development of lymphoma, the most severe complication resulting from PSS. Great efforts are being made to search for new markers that may predict lymphoma in PSS.…”

Section: Introductionmentioning

confidence: 99%

“… 23 , 42 The 14-3-3η proteins are also associated with RA, joint erosion in RA, and secondary Sjogren’s syndrome (SSS) due to systemic lupus erythematosus (SLE). 25 , 43 - 46 …”

Section: Introductionmentioning

confidence: 99%

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14-3-3η Proteins as a Diagnostic Marker, Disease Activation Indicator, and Lymphoma Predictor in Patients with Primary Sjögren Syndrome

Kor,

Yalçın,

Erten

et al. 2023

Arch Iran Med

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Background: Primary Sjögren syndrome (PSS) is a chronic, autoimmune, and lymphoproliferative disease of the connective tissue. In patients with PSS, the risk of developing B-cell non-Hodgkin lymphoma (NHL) increases dramatically, with a prevalence of approximately 5%. The 14-3-3 protein isoforms are phospho-serin/phospho-threonine binding proteins associated with many malignant diseases. This study aimed to evaluate the relationship between disease activity parameters and markers predicting lymphoma development in patients with PSS and 14-3-3η proteins. Methods: This study was designed as an analytical case-control study. A total of 57 PSS patients and 54 healthy volunteers were included in the study. The European League Against Rheumatism (EULAR) Sjögren syndrome disease activity index (ESSDAI) was used to assess systemic disease activity in PSS. Receiver operating characteristic (ROC) analysis was used to test the diagnostic accuracy measures of the analytical results. Multivariable linear regression analysis was used to evaluate the effects of independent variables on the 14-3-3η protein. Results: The 14-3-3η protein serum levels were found to be significantly higher in PSS (2.72 [2.04-4.07]) than healthy controls (1.73 [1.41-2.43]) (P<0.0001). A significant relationship was found between 14-3-3η protein levels and ESSDAI group (β=0.385, 95%CI=0.318-1.651, P=0.005), hypocomplementemia (C3 or C4) (β=0.223, 95% CI=0.09-1.983, P=0.048) and purpura (β=0.252, 95% CI=0.335-4.903, P=0.022), which are accepted as lymphoma predictors. A significant correlation was found between PSS disease activity score ESSDAI and 14-33η protein (β=0.496, 95% CI=0.079-0.244, P=0.0002). Conclusion: 14-3-3η proteins are potential candidates for diagnostic marker, marker of disease activity, and predictor of lymphoma in PSS patients.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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14-3-3η Proteins as a Diagnostic Marker, Disease Activation Indicator, and Lymphoma Predictor in Patients with Primary Sjögren Syndrome

Kor,

Yalçın,

Erten

et al. 2023

Arch Iran Med

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“…Tear proteins detected with the highest number of protein-protein interactions from IPA include 14-3-3 protein zeta/delta (YWHAZ), fibronectin (FN1), and heat shock protein family A members 8 (HSPA8) and 5 (HSPA5). High expression of the 14-3-3 family has been associated with Sjögren's syndrome, although their exact role in the eye is still unclear [66]. Additionally, the expression of both YWHAZ and HSPA8 has been shown to negatively correlate with fluorescein tear break-up time (FTBUT), suggesting these proteins may play a role in dry eye symptoms [66].…”

Section: Discussionmentioning

confidence: 99%

“…High expression of the 14-3-3 family has been associated with Sjögren's syndrome, although their exact role in the eye is still unclear [66]. Additionally, the expression of both YWHAZ and HSPA8 has been shown to negatively correlate with fluorescein tear break-up time (FTBUT), suggesting these proteins may play a role in dry eye symptoms [66]. HSPA5 levels have been shown to increase in tears in response to successful glaucoma treatment [66].…”

Section: Discussionmentioning

confidence: 99%

Comparison of Different Mass Spectrometry Workflows for the Proteomic Analysis of Tear Fluid

Jones

Lee

Glass

et al. 2022

IJMS

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The tear film is a multi-layer fluid that covers the corneal and conjunctival epithelia of the eye and provides lubrication, nutrients, and protection from the outside environment. Tear fluid contains a high concentration of proteins and has thus been recognized as a potential source of biomarkers for ocular disorders due to its proximity to disease sites on the ocular surface and the non-invasive nature of its collection. This is particularly true in the case of dry eye disease, which directly impacts the tear film and its components. Proteomic analysis of tear fluid is challenging mainly due to the wide dynamic range of proteins and the small sample volumes. However, recent advancements in mass spectrometry have revolutionized the field of proteomics enabling unprecedented depth, speed, and accuracy, even with small sample volumes. In this study using the Orbitrap Fusion Tribrid mass spectrometer, we compared four different mass spectrometry workflows for the proteomic analysis of tear fluid collected via Schirmer strips. We were able to establish a method of in-strip protein digestion that identified >3000 proteins in human tear samples from 11 healthy subjects. Our method offers a significant improvement in the number of proteins identified compared to previously reported methods without pooling samples.

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14–3-3 η ETA protein as a potential marker of joint damage in gout

Doğan

Kor

Güven

et al. 2023

Clinical Biochemistry

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Serum 14-3-3η protein is associated with clinical and serologic features of Sjögren’s syndrome in patients with systemic lupus erythematosus: a cross-sectional analysis

Cited by 6 publications

References 27 publications

14-3-3η Proteins as a Diagnostic Marker, Disease Activation Indicator, and Lymphoma Predictor in Patients with Primary Sjögren Syndrome

14-3-3η Proteins as a Diagnostic Marker, Disease Activation Indicator, and Lymphoma Predictor in Patients with Primary Sjögren Syndrome

Comparison of Different Mass Spectrometry Workflows for the Proteomic Analysis of Tear Fluid

14–3-3 η ETA protein as a potential marker of joint damage in gout

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