Sertraline inhibits stress-induced tumor growth through regulating CD8+ T cell-mediated anti-tumor immunity

Abstract

Materials and methods Chemicals and reagentsSertraline was purchased from MedChemExpress, USA; serotonin and Collagenase I were purchased from Sigma, USA. DNase I was purchased from BIOFROXX, USA; Collagenase IV was purchased from Gibco, USA.

Neurotransmitters: promising immune modulators in the tumor microenvironment

“…Sertraline recovered the T cell stressinduced deficiency, including strengthening the infiltration of CD8(+) T cells in the TME, upregulating the expression of IFN-g and GzmB, and reducing the expression of PD-1 on CD8(+) T cells (199). Fluoxetine reduced macrophage polarization in vivo by reversing tumorinduced oxidative damage to macrophages and consequent oxidative stress in thymocytes (196,197). Fluvoxamine significantly suppressed the migration and proliferation of tumor cells and prompted infiltration of T lymphocytes and M1-type macrophages with reduced expression of PD-L1 in colon cancer murine models (198).…”

“…Moreover, fluvoxamine significantly suppressed the migration and proliferation of tumor cells and prompted infiltration of T lymphocytes and M1-type macrophages with reduced PD-L1 molecules in colon cancer murine models (196). Sertraline recovered the T cell stress-induced deficiency by strengthening CD8(+) T cell infiltration, upregulating IFN-g and Granular enzyme B(GzmB) levels, and reducing PD-1 on CD8(+) T cells, indicating its potential to raise the efficacy of ICB immunotherapy (197).…”