SERS speciation of the electrochemical oxidation–reduction of riboflavin

Bailey, Matthew R.; Schultz, Zachary D.

doi:10.1039/c6an01054g

Cited by 23 publications

(7 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Raman analysis was focused on these two regions (Figure 4d,e). The crystal part presents the typical sharp peaks of crystal structures, with peaks attributable to the characteristic signals of riboflavin (750, 1345, 1410 cm −1 ) and ascorbic acid (605 and 632 cm −1 ) (Figure 4d), both present in the MD product [25,26]. The fibrillary part was mainly composed of collagen due to the presence of characteristic peaks at 536, 858, 919, 1065, 1343, 1454 and 1674 cm −1 (Figure 4e) [27].…”

Section: Analysis and Characterization Of The Coatingmentioning

confidence: 99%

The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes

Randelli

Sartori

Carlomagno

et al. 2020

Cells

View full text Add to dashboard Cite

Mechanotransduction is the ability of cells to translate mechanical stimuli into biochemical signals that can ultimately influence gene expression, cell morphology and cell fate. Tenocytes are responsible for tendon mechanical adaptation converting mechanical stimuli imposed during mechanical loading, thus affecting extracellular matrix homeostasis. Since we previously demonstrated that MD-Tissue, an injectable collagen-based medical compound containing swine-derived collagen as the main component, is able to affect tenocyte properties, the aim of this study was to analyze whether the effects triggered by MD-Tissue were based on mechanotransduction-related mechanisms. For this purpose, MD-Tissue was used to coat Petri dishes and cytochalasin B was used to deprive tenocytes of mechanical stimulation mediated by the actin cytoskeleton. Cell morphology, migration, collagen turnover pathways and the expression of key mechanosensors were analyzed by morphological and molecular methods. Our findings confirm that MD-Tissue affects collagen turnover pathways and favors cell migration and show that the MD-Tissue-induced effect represents a mechanical input involving the mechanotransduction machinery. Overall, MD-Tissue, acting as a mechanical scaffold, could represent an effective medical device for a novel therapeutic, regenerative and rehabilitative approach to favor tendon healing in tendinopathies.

show abstract

Section: Analysis and Characterization Of The Coatingmentioning

confidence: 99%

The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes

Randelli

Sartori

Carlomagno

et al. 2020

Cells

View full text Add to dashboard Cite

show abstract

“…This signal is consistent with previous reports of SERS spectra of riboflavin as characteristic peaks are observed at 738 cm -1 (C-C bending of benzene ring), 1158 cm -1 (ring bending of pyrazine and pyrimidine rings), 1250 cm -1 (C=O bending, pyrimidine ring stretch), 1313 cm -1 (C-N-C stretch of pyrazine ring), 1353 cm -1 (C-N stretching of pyrazine ring), 1462 cm -1 (C-N, C-C stretching of pyrazine and pyrimidine rings), and 1525 cm -1 (stretching of benzene ring). [50][51][52] Additional methods of cleaning bare substrates are shown in the Supplemental Material. Figure S6 (Supplemental Material) shows bare substrates that were cleaned using argon plasma and water, however, these were not effective at removing the contamination as the SERS signal from contaminants was still observed.…”

Section: Resultsmentioning

confidence: 99%

Bleach Cleaning of Commercially Available Gold Nanopillar Arrays for Surface-Enhanced Raman Spectroscopy (SERS)

Morder,

Schorr,

Balss

et al. 2023

Appl Spectrosc

Self Cite

View full text Add to dashboard Cite

Surface-enhanced Raman spectroscopy (SERS) is a highly sensitive technique that can assist in trace analysis for biomedical, diagnostic, and environmental applications. However, a major limitation of SERS is surface contamination of the substrates used, which can complicate the spectral reproducibility, limits of detection, and detection of unknown analytes. This is especially prevalent with commercially available substrates as shipping under a controlled and clean environment is difficult. Here we report a method using dilute bleach solutions to remove surface contamination from commercially available substrates consisting of gold-coated nanopillar arrays that maintains functionality. The results show that this method can be used to remove background signals associated with typical surface contamination in commercially available substrates as well as remove thiolated self-assembled monolayers (SAMs). Results indicate the bleach oxidizes the surface contaminants, which can then be easily washed away. Although the metallic surface also becomes oxidized in this process, the surface can be reduced without loss of SERS activity. The SERS intensity of SAMs improved following bleach treatment across all concentrations studied.

show abstract

“…This is a challenging but feasible goal, given that oxidized and reduced flavins can be differentiated according to their spectroscopic signatures. Raman spectroscopy, for example, has been used to detect changes in the spectral features of riboflavin during electrochemical measurements (Bailey & Schultz, 2016). In combination with studies of mutants, such an integrated spectroelectrochemical approach could link the genetic basis of electrode taxis to both the driving molecular gradient and cellular motility response.…”

Section: Future Directionsmentioning

confidence: 99%

Electrolocation? The evidence for redox‐mediated taxis in Shewanella oneidensis

Starwalt-Lee

El‐Naggar

Bond

et al. 2020

Molecular Microbiology

View full text Add to dashboard Cite

Shewanella oneidensis is a dissimilatory metal reducing bacterium and model for extracellular electron transfer (EET), a respiratory mechanism in which electrons are transferred out of the cell. In the last 10 years, migration to insoluble electron acceptors for EET has been shown to be nonrandom and tactic, seemingly in the absence of molecular or energy gradients that typically allow for taxis. As the ability to sense, locate, and respire electrodes has applications in bioelectrochemical technology, a better understanding of taxis in S. oneidensis is needed. While the EET conduits of S. oneidensis have been studied extensively, its taxis pathways and their interplay with EET are not yet understood, making investigation into taxis phenomena nontrivial. Since S. oneidensis is a member of an EET‐encoding clade, the genetic circuitry of taxis to insoluble acceptors may be conserved. We performed a bioinformatic analysis of Shewanella genomes to identify S. oneidensis chemotaxis orthologs conserved in the genus. In addition to the previously reported core chemotaxis gene cluster, we identify several other conserved proteins in the taxis signaling pathway. We present the current evidence for the two proposed models of EET taxis, “electrokinesis” and flavin‐mediated taxis, and highlight key areas in need of further investigation.

show abstract

SERS speciation of the electrochemical oxidation–reduction of riboflavin

Cited by 23 publications

References 49 publications

The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes

The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes

Bleach Cleaning of Commercially Available Gold Nanopillar Arrays for Surface-Enhanced Raman Spectroscopy (SERS)

Electrolocation? The evidence for redox‐mediated taxis in Shewanella oneidensis

Contact Info

Product

Resources

About