SERS Labels for Red Laser Excitation: Silica‐Encapsulated SAMs on Tunable Gold/Silver Nanoshells

Küstner, Bernd; Gellner, Magdalena; Schütz, Max; Schöppler, Friedrich; Marx, Alexander; Ströbel, Philipp; Adam, Patrick; Schmuck, Carsten; Schlücker, Sebastian

doi:10.1002/anie.200804518

Cited by 213 publications

(228 citation statements)

References 31 publications

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“…1B and Scheme 1A). [17][18][19][20] The benets of using MEG-OH and TEG-CO 2 H as hydrophilic spacers attached to aromatic Raman labels include the increased stability of the SERS labels due to the EG termini and the controllable bioconjugation via the MEG-OH/TEG-CO 2 H stoichiometry, together with the advantages of a SAM (maximum surface coverage with Raman reporters, uniform molecular orientation, and minimal coadsorption of other molecules from the surrounding onto the metal surface). SERS immunoassays have been reported for the detection of cell surface antigens, 21 immunoglobulin G (IgG), 8,13 protein A,…”

Section: Introductionmentioning

confidence: 99%

Microspectroscopic SERS detection of interleukin-6 with rationally designed gold/silver nanoshells

Wang

Salehi

Schütz

et al. 2013

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Rationally designed gold/silver nanoshells (Au/Ag-NS) with plasmon resonances optimized for red laser excitation in order to minimize autofluorescence from clinical samples exhibit scattering cross-sections, which are ca. one order of magnitude larger compared with solid quasi-spherical gold nanoparticles (Au-NPs) of the same size. Hydrophilic stabilization and sterical accessibility for subsequent bioconjugation of Au/Ag-NS is achieved by coating their surface with a self-assembled monolayer (SAM) of rationally designed Raman reporter molecules comprising terminal mono-and tri-ethylene glycol (EG) spacers, respectively. The stability of the hydrophilically stabilized metal colloid was tested under different conditions. In contrast to metal colloids coated with a SAM without terminal EG spacers, the hydrophilically stabilized SERS particles do not aggregate under physiologically relevant conditions, i.e., buffer solutions with high ionic strength. Using these rationally designed SERS particles in conjunction with a microspectroscopic acquisition scheme, a sandwich immunoassay for the sensitive detection of interleukin-6 (IL-6) was developed. Several control experiments demonstrate the high specificity of the assay towards IL-6, with a lowest detectable concentration of ca. 1 pg mL À1 . The signal strength of the Au/Ag-NS is at least one order of magnitude higher compared with hydrophilically stabilized, non-aggregated solid quasi-spherical Au-NPs of the same size.

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Section: Introductionmentioning

confidence: 99%

Microspectroscopic SERS detection of interleukin-6 with rationally designed gold/silver nanoshells

Wang

Salehi

Schütz

et al. 2013

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“…In general, they can involve one or more nanoparticles conjugated to (1) a highly polarizable Raman reporter molecule, (2) an affinity ligand such as an aptamer or antibody, and (3) a steric or electrostatic capping agent for stabilization in high salt environments [150,151]. Oligonucleotides, antibodies, protein antigens, small molecules, and dyes can all be immobilized onto metallic nanoparticles using thiol end groups, bifunctional PEG linkers, or sequential click chemistry [152][153][154][155]. Extensive work has been done by groups such as Graham et al who utilize various oligonucleotide and resonant dye-coated nanoprobes to form SERRS active nanoassembly complexes for multiplexed DNA detection [156].…”

Section: Molecularly Mediated Colloidal Sersmentioning

confidence: 99%

Surface enhanced Raman spectroscopy (SERS) for in vitro diagnostic testing at the point of care

et al. 2017

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Point-of-care (POC) device development is a growing field that aims to develop low-cost, rapid, sensitive in-vitro diagnostic testing platforms that are portable, selfcontained, and can be used anywhere -from modern clinics to remote and low resource areas. In this review, surface enhanced Raman spectroscopy (SERS) is discussed as a solution to facilitating the translation of bioanalytical sensing to the POC. The potential for SERS to meet the widely accepted "ASSURED" (Affordable, Sensitive, Specific, Userfriendly, Rapid, Equipment-free, and Deliverable) criterion provided by the World Health Organization is discussed based on recent advances in SERS in vitro assay development. As SERS provides attractive characteristics for multiplexed sensing at low concentration limits with a high degree of specificity, it holds great promise for enhancing current efforts in rapid diagnostic testing. In outlining the progression of SERS techniques over the past years combined with recent developments in smart nanomaterials, high-throughput microfluidics, and low-cost paper diagnostics, an extensive number of new possibilities show potential for translating SERS biosensors to the POC.

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“…[25][26][27][28] In SERS NP labels/nanotags, [17][18][19][20][21][22][23][24][25][26][27][28] Raman labels/reporter molecules are permanently adsorbed onto the surface of the metal colloid (Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

“…24,38,39 First, solid silver NPs are produced in a polyol process, using silver nitrate dissolved in ethylene glycol together with polyvinylpyrrolidone (PVP) as both reducing and stabilizing agent. The silver NPs then serve as a template for the formation of hollow Au/Ag nanoshells upon addition of Au 3+ ions.…”

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confidence: 99%

Rational design and synthesis of SERS labels

Wang

Schlücker

2013

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SERS labels are a new class of nanotags for optical detection based on Raman scattering. Central advantages include their spectral multiplexing capacity due to the small line width of vibrational Raman bands, quantification based on spectral intensities, high photostability, minimization of autofluorescence from biological specimens via red to near-infrared (NIR) excitation, and the need for only a single laser excitation line. Current concepts for the rational design and synthesis of SERS labels are summarized in this review. Chemical constituents of SERS labels are the plasmonically active metal colloids for signal enhancement upon resonant laser excitation, organic Raman reporter molecules for adsorption onto the metal surface for identification, and an optional protective shell. Different chemical approaches towards the synthesis of rationally designed SERS labels are highlighted, including also their subsequent bioconjugation.

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SERS Labels for Red Laser Excitation: Silica‐Encapsulated SAMs on Tunable Gold/Silver Nanoshells

Cited by 213 publications

References 31 publications

Microspectroscopic SERS detection of interleukin-6 with rationally designed gold/silver nanoshells

Microspectroscopic SERS detection of interleukin-6 with rationally designed gold/silver nanoshells

Surface enhanced Raman spectroscopy (SERS) for in vitro diagnostic testing at the point of care

Rational design and synthesis of SERS labels

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