SERS-ELISA using silica-encapsulated Au core-satellite nanotags for sensitive detection of SARS-CoV-2

Yu, Qian; Trinh, Hoa Duc; Lee, Yeonji; Kang, Taejoon; Chen, Lingxin; Yoon, Sangwoon; Choo, Jaebum

doi:10.1016/j.snb.2023.133521

Cited by 22 publications

(19 citation statements)

References 51 publications

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“…As shown in Figure , AuNPs formed a uniform self-assembly via butanol dehydration, and Cy3-DNAs were enriched around the AuNPs. The SERS enhancement and DNA enrichment factors were estimated to increase by 10 7 and 280, , respectively, due to butanol dehydration. Therefore, the DNA–AuNP assembly prepared through butanol dehydration can be used as a SERS detection platform for highly sensitive DNA assays.…”

Section: Resultsmentioning

confidence: 99%

Highly Uniform Self-Assembly of Gold Nanoparticles by Butanol-Induced Dehydration and Its SERS Applications in SARS-CoV-2 Detection

Wu,

Yu,

Joung

et al. 2023

Anal. Chem.

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We report the development of a reproducible and highly sensitive surface-enhanced Raman scattering (SERS) substrate using a butanol-induced self-assembly of gold nanoparticles (AuNPs) and its application as a rapid diagnostic platform for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The butanol-induced self-assembly process was used to generate a uniform assembly of AuNPs, with multiple hotspots, to achieve high reproducibility. When an aqueous droplet containing AuNPs and target DNAs was dropped onto a butanol droplet, butanol-induced dehydration occurred, enriching the target DNAs around the AuNPs and increasing the loading density of the DNAs on the AuNP surface. The SERS substrate was evaluated by using Raman spectroscopy, which showed strong electromagnetic enhancement of the Raman signals. The substrate was then tested for the detection of SARS-CoV-2 using SERS, and a very low limit of detection (LoD) of 3.1 × 10–15 M was obtained. This provides sufficient sensitivity for the SARS-CoV-2 screening assay, and the diagnostic time is significantly reduced as no thermocycling steps are required. This study demonstrates a method for the butanol-induced self-assembly of AuNPs and its application as a highly sensitive and reproducible SERS substrate for the rapid detection of SARS-CoV-2. The results suggest the potential of this approach for developing rapid diagnostic platforms for other biomolecules and infectious diseases.

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Section: Resultsmentioning

confidence: 99%

Highly Uniform Self-Assembly of Gold Nanoparticles by Butanol-Induced Dehydration and Its SERS Applications in SARS-CoV-2 Detection

Wu,

Yu,

Joung

et al. 2023

Anal. Chem.

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“…We have successfully demonstrated a similar scheme using AuNS@AuNS for the detection of SARS-CoV-2. 21 The extraordinarily large SERS signal intensity from AuNC@ AuNC is expected to lower the detection limit, thereby significantly increasing sensitivity. We believe that our innovative assembly method and the combinatorial approach to selecting the most effective plasmonic nanoassembly combinations will make a great contribution to the advancement of nanotechnology.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Realizing the highest level of enhancement is a critical factor in the practical implementation of SERS, as it directly affects the sensitivity of sensing and diagnostic devices based on plasmonic nanoparticles . In this context, nanoparticle assemblies offer a highly promising architectural platform for achieving a significant SERS enhancement primarily due to the presence of nanogaps within their structure.…”

Section: Introductionmentioning

confidence: 99%

Combinatorial Approach to Find Nanoparticle Assemblies with Maximum Surface-Enhanced Raman Scattering

Trinh,

Kim,

Yun

et al. 2023

ACS Appl. Mater. Interfaces

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Plasmonic nanoparticles exhibit unique properties that distinguish them from other nanomaterials, including vibrant visible colors, the generation of local electric fields, the production of hot charge carriers, and localized heat emission. These properties are particularly enhanced in the narrow nanogaps formed between nanostructures. Therefore, creating nanogaps in a controlled fashion is the key to achieving a fundamental understanding of plasmonic phenomena originating from the nanogaps and developing advanced nanomaterials with enhanced performance for diverse applications. One of the most effective approaches to creating nanogaps is to assemble individual nanoparticles into a clustered structure. In this study, we present a fast, facile, and highly efficient method for preparing core@satellite (CS) nanoassembly structures using gold nanoparticles of various shapes and sizes, including nanospheres, nanocubes (AuNCs), nanorods, and nanotriangular prisms. The sequential assembly of these building blocks on glass substrates allows us to obtain CS nanostructures with a 100% yield within 4 h. Using 9 different building blocks, we successfully produce 16 distinct CS nanoassemblies and systematically investigate the combinations to search for the highest Raman enhancement. We find that the surface-enhanced Raman scattering (SERS) intensity of AuNC@AuNC CS nanoassemblies is 2 orders of magnitude larger than that of other CS nanoassemblies. Theoretical analyses reveal that the intensity and distribution of the electric field induced in the nanogaps by plasmon excitation, as well as the number of molecules in the interfacial region, collectively contribute to the unprecedentedly large SERS enhancement observed for AuNC@AuNC. This study not only presents a novel assembly method that can be extended to produce many other nanoassemblies but also identifies a highly promising SERS material for sensing and diagnostics through a systematic search process.

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“…According to previous studies, core–satellite nanoassemblies have been widely used as SERS probes for the detection of biomolecules such as biotoxins, SARS-CoV-2, etc. by virtue of their multiple hot spots between the core and satellite nanoparticles on a structural unit as well as narrow nanogaps of each hot spot for significantly enhanced SERS signals. − Considering the high sensitivity of SERS, the high spatial resolution of SPR, and the targeting specificity of aptamers, here, we develop aptamer-based core–satellite plasmonic nanoassemblies as SPR/SERS dual-mode sensors for the sensitive detection of RAC in complex media. In the core–satellite nanoassemblies, gold nanoparticles of 15 nm in diameter acting as the satellites (sGNPs) were comodified by RAC aptamer and 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB), and gold nanoparticles of 62 nm in diameter acting as the core (cGNPs) were comodified by complementary DNA (cDNA) and 4-mercaptobenzoic acid (4MBA) (Scheme A).…”

Section: Introductionmentioning

confidence: 99%

Core–Satellite Nanoassemblies as SPR/SERS Dual-Mode Plasmonic Sensors for Sensitively Detecting Ractopamine in Complex Media

Zheng,

Hu,

Zhao

et al. 2023

J. Agric. Food Chem.

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SERS-ELISA using silica-encapsulated Au core-satellite nanotags for sensitive detection of SARS-CoV-2

Cited by 22 publications

References 51 publications

Highly Uniform Self-Assembly of Gold Nanoparticles by Butanol-Induced Dehydration and Its SERS Applications in SARS-CoV-2 Detection

Highly Uniform Self-Assembly of Gold Nanoparticles by Butanol-Induced Dehydration and Its SERS Applications in SARS-CoV-2 Detection

Combinatorial Approach to Find Nanoparticle Assemblies with Maximum Surface-Enhanced Raman Scattering

Core–Satellite Nanoassemblies as SPR/SERS Dual-Mode Plasmonic Sensors for Sensitively Detecting Ractopamine in Complex Media

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