SERS detection of indirect viral DNA capture using colloidal gold and methylene blue as a Raman label

Gold Nanoparticles in Chemical and Biological Sensing

“…have demonstrated an indirect capture model assay for SERS-based detection of DNA using AuNPs. 993 SERS based approach-utilizing AuNP probes for identifying and quantifying multiple gene segments extracted from cells has been described by Irudayaraj et al . 994 Multiple AuNP-on-wire systems as a SERS sensing platform for viral DNA have been developed by Kang and coworkers.…”

A versatile SERS-based immunoassay for immunoglobulin detection using antigen-coated gold nanoparticles and malachite green-conjugated protein A/G

“…The real-time monitoring of WNV E antigen deposition onto Au plated quartz sensor crystals and subsequent interactions with Rb anti-WNV E and MG-pA/G was conducted using conditions similar to those described elsewhere (Harpster et al, 2009). Briefly, sensor crystals were washed with antigen solubilization buffer containing urea, followed by the delivery of 30 g/ml of antigen in the same buffer until changes in resonant frequency ( f) plateaued.…”

“…Research developments in biosensor applications for surface enhanced Raman spectroscopy (SERS) have demonstrated a broad range of solutions for the sensitive and selective detection of nucleic acids (Cao et al, 2002;Hering et al, 2008;Harpster et al, 2009), proteins (Xu et al, 2005;Porter et al, 2008;Han et al, 2009a), protein-DNA interactions (Bonham et al, 2007) and in vivo tumor tissue (Qian et al, 2008). Although technical strategies can vary widely, SERS-based assays for analyte detection are largely predicated on the interdependence of target molecule capture and positioning of an appropriate Raman scattering label, at, or in close proximity to roughened noble metal surfaces.…”

Self-assembled plasmonic templates produced by microwave annealing: applications to surface-enhanced Raman scattering

“…In particular, the study of the Localized Surface Plasmon Resonance (LSPR), a collective oscillation of the metals' conduction electrons accompanied by extreme near-field enhancement, led to myriads of publications and promises groundbreaking applications in everyday life, such as biosensing [1,2], photonic and microelectronic devices [3][4][5], and optical storage of information [6]. Among these applications, surface-enhanced Raman scattering (SERS) and surfaceenhanced resonant Raman scattering (SERRS) were extensively studied [7][8][9][10][11][12][13] because of both the fundamental science lying behind them, as well as due to the tremendous social impact of their application to early medical diagnosis, as well as to toxicology and forensics [14][15][16].…”