SERS-based ultrafast and sensitive detection of luteinizing hormone in human serum using a passive microchip

Gjergjizi, Belma; Çoğun, Ferah; Yıldırım, Ender; Eryılmaz, Merve; Selbes, Yeşim; Sağlam, Necdet; Tamer, Uğur

doi:10.1016/j.snb.2018.05.001

Cited by 26 publications

(7 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The acute opening and the intersection of the microchannels and the chambers generate a capillary pinning barrier, so that the liquid loaded in a previous chamber does not overflow into the successive chamber. The details of the working principles of the microfluidic chip have been described in our previous works [ 42 , 43 ].…”

Section: Methodsmentioning

confidence: 99%

Escherichia coli Enumeration in a Capillary-Driven Microfluidic Chip with SERS

et al. 2022

Self Cite

View full text Add to dashboard Cite

Pathogen detection is still a challenging issue for public health, especially in food products. A selective preconcentration step is also necessary if the target pathogen concentration is very low or if the sample volume is limited in the analysis. Plate counting (24–48 h) methods should be replaced by novel biosensor systems as an alternative reliable pathogen detection technique. The usage of a capillary-driven microfluidic chip is an alternative method for pathogen detection, with the combination of surface-enhanced Raman scattering (SERS) measurements. Here, we constructed microchambers with capillary microchannels to provide nanoparticle–pathogen transportation from one chamber to the other. Escherichia coli (E. coli) was selected as a model pathogen and specific antibody-modified magnetic nanoparticles (MNPs) as a capture probe in a complex milk matrix. MNPs that captured E. coli were transferred in a capillary-driven microfluidic chip consisting of four chambers, and 4-aminothiophenol (4-ATP)-labelled gold nanorods (Au NRs) were used as the Raman probe in the capillary-driven microfluidic chip. The MNPs provided immunomagnetic (IMS) separation and preconcentration of analytes from the sample matrix and then, 4-ATP-labelled Au NRs provided an SERS response by forming sandwich immunoassay structures in the last chamber of the capillary-driven microfluidic chip. The developed SERS-based method could detect 101–107 cfu/mL of E. coli with the total analysis time of less than 60 min. Selectivity of the developed method was also tested by using Salmonella Enteritidis (S. Enteritidis) and Staphylococcus aureus (S. aureus) as analytes, and very weak signals were observed.

show abstract

Section: Methodsmentioning

confidence: 99%

Escherichia coli Enumeration in a Capillary-Driven Microfluidic Chip with SERS

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…The use of antibiotics and hormones in the industry remains controversial because of their potential negative health effects on consumers (Gjergjizi et al., 2018). The microfluidic systems combined with other detection methods have shown advantages in detecting hormones and antibodies with the simulation software as an auxiliary tool.…”

Section: Simulation Applications In Food Analysismentioning

confidence: 99%

Computer simulation of submicron fluid flows in microfluidic chips and their applications in food analysis

Xie

Sun

2021

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

In recent years, countries around the world have maintained a zero‐tolerance attitude toward safety problems in the food industry. In order to ensure human health, a fast, sensitive, and high‐throughput analysis of food contaminants is necessary to ensure safe food products on the market. Microfluidics, as a high‐efficiency and sensitive detection technology, has many advantages in the detection of food contaminants, including foodborne pathogens, pesticides, heavy metal ions, toxic substances, and so forth, especially in conjunction with a variety of submicron fluid driving methods, making food detection and analysis more efficient and accurate. This review introduces the principle of submicron fluid driving modes and discusses the driving simulation of submicron fluid in microfluidic chips. In addition, the latest developments in the application of simulation in food analysis from 2006 to 2020 are discussed, and the computer simulation of submicron fluid flow in microfluidic chips and its application and development trend in food analysis are also highlighted. The review indicates that microfluidic technology, using numerical simulation as an auxiliary tool, combined with traditional methods has greatly improved the detection and analysis of food products. In addition, microfluidics combined with a variety of control methods embodies the ability of specific, multifunctional, and sensitive detection and analysis of food products. The development of high‐sensitivity, high‐throughput, portable, integrated microfluidic chips will enable the technology to be applied in practice.

show abstract

“…Such SERS biosensors have also been developed for early diagnosis of colorectal [238] and breast cancer [239] as well as for monitoring the therapeutic effects during cancer treatment [240,241]. AuNPs [242,243] and AgNPs [244] have frequently been exploited in SERS-based hormone investigations. Functionalisation of Au shell -Ag core with DNA enhanced the sensitivity of SERS assays for 17β-estradiol by 10-folds [245] in comparison to 4-mercaptobenzoic acid [246].…”

Section: Reflectometric Interference Spectroscopy Biosensorsmentioning

confidence: 99%

Recent Progress in Optical Sensors for Biomedical Diagnostics

Pirzada

Altıntaş

2020

Micromachines

View full text Add to dashboard Cite

In recent years, several types of optical sensors have been probed for their aptitude in healthcare biosensing, making their applications in biomedical diagnostics a rapidly evolving subject. Optical sensors show versatility amongst different receptor types and even permit the integration of different detection mechanisms. Such conjugated sensing platforms facilitate the exploitation of their neoteric synergistic characteristics for sensor fabrication. This paper covers nearly 250 research articles since 2016 representing the emerging interest in rapid, reproducible and ultrasensitive assays in clinical analysis. Therefore, we present an elaborate review of biomedical diagnostics with the help of optical sensors working on varied principles such as surface plasmon resonance, localised surface plasmon resonance, evanescent wave fluorescence, bioluminescence and several others. These sensors are capable of investigating toxins, proteins, pathogens, disease biomarkers and whole cells in varied sensing media ranging from water to buffer to more complex environments such as serum, blood or urine. Hence, the recent trends discussed in this review hold enormous potential for the widespread use of optical sensors in early-stage disease prediction and point-of-care testing devices.

show abstract

SERS-based ultrafast and sensitive detection of luteinizing hormone in human serum using a passive microchip

Cited by 26 publications

References 42 publications

Escherichia coli Enumeration in a Capillary-Driven Microfluidic Chip with SERS

Escherichia coli Enumeration in a Capillary-Driven Microfluidic Chip with SERS

Computer simulation of submicron fluid flows in microfluidic chips and their applications in food analysis

Recent Progress in Optical Sensors for Biomedical Diagnostics

Contact Info

Product

Resources

About