SERS-based immunoassay for monitoring cortisol-related disorders

Grand Challenge in Adrenal Endocrinology: Is the Legacy of the Past a Challenge for the Future of Precision Medicine?

“…Finally, the possibility of measuring cortisol and cortisone with LC-MS/MS can consent to evaluate the degree of peripheral activation of cortisone in cortisol, which could become an important marker to evaluate the cortisol milieu in the individual patient (14,45). Other methods for measuring cortisol will probably be available, such as surface-enhanced Raman spectroscopy, in addition to immunoassay, which has recently been suggested to be even better than LC-MS/MS in terms of sensitivity for in situ monitoring of human stress levels and cortisol-related disorders (46).…”

Stabile SERS Encoded Silver Silica Nanocomposites for Industrial Labeling – The Case of COVID-19 Diagnosis

“…SERS as a non-destructive technology provides chemical information in aqueous environments. Thus SERS has been an effective tool to realize qualitative and quantitative detection of biological species, including micro RNA analysis (11), enzyme (12), hydrogen peroxide (13), staphylococcal enterotoxin B (14) and other diseases biomarkers (15) , (16) with gold nanowire, gold-silver alloy NPs and gold-MnO2 core-shell, hollow gold nanospheres and gold nanostar and nanosphere separately. However, producing homogenous, sensitive and reproducible SERS platforms is the main difficulty which hampers SERS bioapplications (17), with many efforts made in the design and synthesis of uniform nanomaterials (18) , (19) , (20).…”

AuNP array coated substrate for sensitive and homogeneous SERS-immunoassay detection of human immunoglobulin G

“…8 Immunoassay based on the specic interaction between an antigen and antibody is a powerful analytical method, which has become a popular tool for clinical diagnosis. [9][10][11][12] Based on different labelling techniques, many immunoassay methods have been developed for the detection of biomarkers in human serum, such as uorescence immunoassay (FIA), 13 enzymelinked immunosorbent assay (ELISA) 14 and chemiluminescence immunoassay (CLIA). Although effective, these methods still have some limitations.…”

“…Compared with the ordinary glass slide, the Au-sputtered solid substrate provided additional signal enhancement via coupling with SERS tags. 9 In this study, we developed a SERS-immunoassay method for the detection of HIgG. The method includes the preparation of the SERSIA tag with 4-aminothiophenol (4-ATP) as Raman prober (Scheme 1A) and the AuNP array (AuA)-coated solid SERSIA substrate (Scheme 1B).…”