SERS-Based Aptasensor for Rapid Quantitative Detection of SARS-CoV-2

Abstract

During the COVID-19 pandemic, the development of sensitive and rapid techniques for detection of viruses have become vital. Surface-enhanced Raman scattering (SERS) is an appropriate tool for new techniques due to its high sensitivity. SERS materials modified with short-structured oligonucleotides (DNA aptamers) provide specificity for SERS biosensors. Existing SERS-based aptasensors for rapid virus detection are either inapplicable for quantitative determination or have sophisticated and expensive construction and implementation. In this paper, we provide a SERS-aptasensor based on colloidal solutions which combines rapidity and specificity in quantitative determination of SARS-CoV-2 virus, discriminating it from the other respiratory viruses.

Recent Advances and Applications in Paper-Based Devices for Point-of-Care Testing

“…Zavyalova’s group developed a paper-based device for detection of viruses with SERS. SARS-CoV-2 virus can be measured rapidly with better selevtivity [ 264 ]. In 2017, a plasmonic filter was used by Wang’s group to filtrate, capture and identify Streptomycete spores with SERS method.…”

Aptameric nanobiosensors for the diagnosis of COVID-19: An update

“…A comprehensive overview of the aptameric nanosensors developed for COVID-19 diagnosis are given in Table 1 . Vlamir et al devised an aptamer-based nanosensor containing silver nanoparticles as SERS substrate to detect SARS-CoV-2 ( Figure 1 ) [11] . It is a one-step protocol that demonstrated aggregation of silver nanoparticles after the binding of labeled aptamer with incubated virus and also showed an intense SERS signal at 587 cm -1 .…”

“… Sensor Principle Aptamer Nanomaterials Recognition element (SARS-CoV-2) Analytical Sensitivity Limit of Detection References 1. Surface Enhanced Raman Scattering Biotinylated DNA aptamer Silver Nanoparticles Inactivated whole virus 5.5 x 10 4 to 1.4 x 10 6 TCID 50 /mL 5.5 x 10 4 TCID 50 /mL [11] 2. SLIP- Surface Enhanced Raman Scattering Thiolated DNA aptamer Silver Nanoparticles Spike protein 1 nM to 1 pM 1 fM [12] 3.…”

Nanobiotechnology enabled approaches for wastewater based epidemiology

“…SERS spectra of adenovirus, rhinovirus, and human immunodeficiency virus (HIV) were collected previously by dropping small volumes (0.5-1 μL) of these viruses on a substrate consisting of Ag nanorod arrays [ 39 ]. A SERS-based aptasensor was developed by functionalizing colloidal AgNPs with olegonucleotides for detection of SARS-CoV-2 in water at 5.5 × 10 4 TCID50/mL level [ 40 ]. A portable handheld Raman system was used to detect influenza A virus using 10 μL of sample in water applied to Ag nanorod substrates [ 41 ].…”