SERS-active Au/SiO_2 clouds in powder for rapid ex vivo breast adenocarcinoma diagnosis

Abstract

Abstract:In the present work, we report a dry-based application technique of Au/SiO 2 clouds in powder for rapid ex vivo adenocarcinoma diagnosis through surface-enhanced Raman scattering (SERS); using low laser power and an integration time of one second. Several characteristic Raman peaks frequently used for the diagnosis of breast adenocarcinoma in the range of the amide III are successfully enhanced by breading the tissue with Au/SiO 2 powder. The SERS activity of these Au/SiO 2 powders is attributed to their rapid rehydration upon contact with the wet tissues, which promotes the formation of gold nanoparticle aggregates. The propensity of the Au/SiO 2 cloud structures to adsorb biomolecules in the vicinity of the gold nanoparticle clusters promotes the necessary conditions for SERS detection. In addition, electron microscopy, together with elemental analysis, have been used to confirm the structure of the new Au/SiO 2 cloud material and to investigate its distribution in breast tissues. References and links1. R. Manoharan, Y. Wang, and M. S. Feld, "Histochemical analysis of biological tissues using Raman spectroscopy," Spectrochim. Acta A Mol. Biomol. Spectrosc. 52(2), 215-249 (1996). 2. Y. Liu, H. Miyoshi, and M. Nakamura, "Nanomedicine for drug delivery and imaging: a promising avenue for cancer therapy and diagnosis using targeted functional nanoparticles," Int. J. Cancer 120(12), 2527-2537 (2007). 3. J. Oh, M. D. Feldman, J. Kim, C. Condit, S. Emelianov, and T. E. Milner, "Detection of magnetic nanoparticles in tissue using magneto-motive ultrasound," Nanotechnology 17(16), 4183-4190 (2006) A. Walton, J. D. Brenton, and C. Caldas, "Astronomical algorithms for automated analysis of tissue protein expression in breast cancer," Br.

Raman spectroscopy: techniques and applications in the life sciences

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Superhydrophobic silver film as a SERS substrate for the detection of uric acid and creatinine

“…Surface-enhanced Raman scattering (SERS) [6,7] is a sensitive spectroscopic technique capable of providing a characteristic molecular vibrational fingerprint, even at trace concentrations. Since the first report on SERS in 1977, many efforts have been made to achieve higher sensitivity in chemical and biological detection [8,9]. It is generally regarded that SERS enhancement stems from two mechanism [10,11]: One is chemical enhancement, which is realized by charge transfer between the analytes and the SERS substrate; the other is electromagnetic enhancement, which plays the dominant role in SERS enhancement [12,13].…”

Imaging and SERS Study of the Au Nanoparticles Interaction with HPV and Carcinogenic Cervical Tissues

“…The search for new diagnostic tools with better specificity and sensitivity has led to the consideration of different spectroscopic techniques capable of detection at the molecular level. Over the past years, Raman spectroscopy has provided excellent results in the diagnosis of a wide range of cancers, including breast, prostate, esophageal, colon, lung, oral and cervical [ 8 , 9 , 10 , 11 , 12 , 13 , 14 ]. Raman spectroscopy can be implemented microscopically to study cellular components such as nucleus, nucleoli, or chromatin [ 10 , 15 ]; due to its high spatial resolution, specific biomolecular complexes such as phenylalanine, tyrosine, and adenine can be analyzed [ 16 , 17 , 18 ].…”