Serratia marcescens resistance profile and its susceptibility to photodynamic antimicrobial chemotherapy

Parente, Ticiana Mont Alverne Lopes; Rebouças, Emanuela de Lima; Santos, Vitor Coutinho Vieira dos; Barbosa, Francisco César Barroso; Zanin, Iriana Carla Junqueira

doi:10.1016/j.pdpdt.2016.04.007

Cited by 13 publications

(8 citation statements)

References 22 publications

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“…A high proportion of these species of bacteria are known to show intrinsic or acquired resistance to many antimicrobial drugs, including those commonly used in initial treatment protocols for foals with sepsis. [24][25][26][27][28][29] These bacterial species were responsible for a large proportion of the HAIs in a large study in humans: Enterococcus spp., 13.9%; Klebsiella spp., 8%; Pseudomonas spp., 7.5%; Serratia spp., 2.1%; and Acinetobacter spp., 1.8%. 30 In equine medicine, there also are several reports on the role of these bacteria in HAIs.…”

Section: Isolation Frequencymentioning

confidence: 99%

Differences in isolation rate and antimicrobial susceptibility of bacteria isolated from foals with sepsis at admission and after ≥48 hours of hospitalization

Theelen

Wilson

Byrne

et al. 2020

Veterinary Internal Medicne

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Background Antimicrobial treatment protocols for foals with sepsis that do not improve clinically often are adjusted based on bacteriological and antimicrobial susceptibility testing results from samples collected at hospital admission. Objectives To evaluate whether hospitalization for ≥48 hours affects bacteriological and antimicrobial susceptibility testing results. Animals Two‐hundred sixty‐seven foals <30 days of age admitted to a neonatal intensive care unit and diagnosed with sepsis. Methods Medical records were reviewed retrospectively to identify foals with sepsis and positive bacteriological cultures. Results from samples collected at hospital admission were compared to those collected ≥48 hours after admission. Logistic regression for clustered data and exact logistic regression were used for statistical analysis. Results Three‐hundred fifty‐three unique bacterial isolates were obtained from 231 foals at hospital admission and 92 unique bacterial isolates were obtained from 57 foals after ≥48 hours of hospitalization. Relative isolation frequency after ≥48 hours of hospitalization increased for Acinetobacter spp., 0.6% versus 3.3% (odds ratio [OR], 7.63; 95% confidence interval [CI], 1.28‐45.45); Enterococcus spp., 4.8% versus 19.6% (OR, 5.37; 95% CI, 2.64‐10.90); Klebsiella spp., 5.1% versus 10.9% (OR, 2.27; 95% CI, 1.05‐4.89); Pseudomonas spp., 3.0% versus 7.6% (OR, 3.49; 95% CI, 3.49‐240.50); and Serratia spp., 3.0% versus 5.4% (OR, 20.23; 95% CI, 2.20‐186.14). Bacteria isolated after ≥48 hours of hospitalization were less susceptible to all tested antimicrobial drugs, except for imipenem. Conclusions and Clinical Importance Decreased antimicrobial susceptibility of bacteria isolated after ≥48 hours of hospitalization provides a rationale for repeated bacteriological culture and susceptibility testing in hospitalized foals with sepsis.

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Section: Isolation Frequencymentioning

confidence: 99%

Differences in isolation rate and antimicrobial susceptibility of bacteria isolated from foals with sepsis at admission and after ≥48 hours of hospitalization

Theelen

Wilson

Byrne

et al. 2020

Veterinary Internal Medicne

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“…14 While low-level light is an effective inhibitor of microbes, utilisation of photodynamic therapy (PDT) has been shown to have increased microbial inhibition compared to LLLT alone for certain organisms. 15 PDT, the combination of non-toxic photosensitising dyes with focused wavelengths of visible light, produces destructive reactive oxygen species that kill the cell by triggering apoptosis or necrosis. 16 Methylene blue (MB) is a photosensitiser that has a strong absorption band in the red light wavelength range, presents low toxicity towards humans and is relatively inexpensive.…”

Section: Introductionmentioning

confidence: 99%

In vitro fungicidal effects of methylene blue at 625‐nm

Guffey

Payne

Roegge

2017

Mycoses

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The aim of the study is to confirm the effectiveness of photodynamic therapy (PDT) as a significant inhibitor of Trichophyton rubrum (T. rubrum) and to determine the most appropriate dose and rate of delivery. Trichophyton rubrum is the most common dermatophyte worldwide, responsible for the majority of superficial fungal infections. The traditional treatment of T. rubrum has known adverse effects. An alternative treatment is warranted. Photosensitised T. rubrum specimens were treated with 625-nm light at doses of 3, 12, 24, 40 and 60 J/cm . Colony counts were performed and compared to untreated controls. Doses of 24, 40 and 60 J/cm all produced kill rates of over 94%. A lower rate of delivery (7.80 mW/cm ) was shown to be a greater inhibitor of T. rubrum than a higher rate of delivery (120 mW/cm ). Photodynamic therapy with methylene blue (MB) at 625 nm using a low rate of delivery at doses of 24, 40 and 60 J/cm is an effective inhibitor of T. rubrum. A rate of delivery of 7.80 mW/cm is a significantly greater inhibitor of T. rubrum than a rate of 120 mW/cm when applying 625-nm light in PDT using MB.

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“…Interestingly, several Serratia sp. are also antibiotic-resistant bacteria [ 23 ]. Overall, antibiotic-producing bacteria that are capable of inhibiting growth of both gram positive and negative bacteria (i.e.…”

Section: Main Textmentioning

confidence: 99%

Bioprospecting saline gradient of a Wildlife Sanctuary for bacterial diversity and antimicrobial activities

2017

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ObjectiveAntibiotic-resistant bacteria are becoming a global crisis, causing death of thousands of people and significant economic impact. The discovery of novel antibiotics is crucial to saving lives and reducing healthcare costs. To address the antibiotic-resistant crisis, in collaboration the Small World Initiative, which aims to crowdsource novel antibiotic discovery, this study aimed to identify antimicrobial producing bacteria and bacterial diversity in the soil of the Stimpson Wildlife Sanctuary, an inland area with a soil salt gradient.ResultsApproximately 4500 bacterial colonies were screened for antimicrobial activity and roughly 100 bacteria were identified as antimicrobial producers, which belong to Entrococcaceae (74%), Yersiniaceae (19%), and unidentified families (7%). Several bacterial isolates showed production of broad spectrum inhibitory compounds, while others were more specific to certain pathogens. The data obtained from the current study provide a resource for further characterization of the soil bacteria with antimicrobial activity, with an aim to discover novel ones. The study showed no correlation between soil salt level and the presence of bacteria with antimicrobial activities. However, most of the identified antimicrobial producing bacteria do not belong to actinomycetes, the most common phyla of antibiotic producing bacteria and this could potentially lead to the discovery of novel antibiotics.Electronic supplementary materialThe online version of this article (doi:10.1186/s13104-017-2711-9) contains supplementary material, which is available to authorized users.

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Serratia marcescens resistance profile and its susceptibility to photodynamic antimicrobial chemotherapy

Cited by 13 publications

References 22 publications

Differences in isolation rate and antimicrobial susceptibility of bacteria isolated from foals with sepsis at admission and after ≥48 hours of hospitalization

Differences in isolation rate and antimicrobial susceptibility of bacteria isolated from foals with sepsis at admission and after ≥48 hours of hospitalization

In vitro fungicidal effects of methylene blue at 625‐nm

Bioprospecting saline gradient of a Wildlife Sanctuary for bacterial diversity and antimicrobial activities

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