Serratia marcescens Induces Apoptotic Cell Death in Host Immune Cells via a Lipopolysaccharide- and Flagella-dependent Mechanism

Ishii, Kenichi; Adachi, Tatsuo; Imamura, Katsutoshi; Takano, Shinya; Usui, Kimihito; Suzuki, Kazushi; Hamamoto, Hiroshi; Watanabe, Takeshi; Sekimizu, Kazuhisa

doi:10.1074/jbc.m112.399667

Cited by 45 publications

(35 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1B). We and others have demonstrated that fewer than 10 colony-forming units of S. marcescens kills insects, including silkworms (15,25). We therefore hypothesized that the above phenomenon, suppression of the injurydependent reduction in hemocyte numbers by S. marcescens, is responsible for the high virulence of S. marcescens against silkworms.…”

Section: Decrease In Silkworm Hemocyte Number By Injury Stimulation Amentioning

confidence: 94%

“…In the course of our studies of S. marcescens pathogenesis using the silkworm infection model (15), we found that the number of freely circulating hemocytes in silkworms increased immediately after infection. We speculated that the bacteria inhibited the adhesion of hemocytes to the body cavity and tissues.…”

mentioning

confidence: 91%

“…Strips were then subjected to SDS-PAGE using 12.5% gels (12 ϫ 14 cm), and the gels were stained with Coomassie Brilliant Blue. For analysis of mouse peritoneal macrophages, cells were obtained from C57BL/6JJc1 female mice as described previously (15). Approximately 0.5 ϫ 10 8 cells obtained from 15 mice were treated with serralysin, and the membrane fraction was prepared and analyzed as described above.…”

mentioning

confidence: 99%

“…Construction of S. marcescens-disrupted Mutant of the ser Gene-Gene disruption of S. marcescens was performed according to previous reports (15,21,22). Briefly, the 145-309th nucleotides of the internal region within the ser open reading frame (1536 bp) was amplified by PCR using forward (5Ј-AAGAATTCGACG-ACCTGCTGCATTATCA-3Ј) and reverse (5Ј-AAGAATTCAT-CCGGGAAGGAGAAGGTTA-3Ј) primers and S. marcescens 2170 genome DNA as a template.…”

mentioning

confidence: 99%

“…LD 50 values (at 16 h post-infection of S. marcescens or 24-h post-infection of S. aureus) were determined as described previously (15). In the mouse infection experiments, C57BL/6JJc1 female mice (7-8 weeks old) were intravenously injected with 200 l of S. marcescens (3 ϫ 10…”

mentioning

confidence: 99%

See 4 more Smart Citations

Serratia marcescens Suppresses Host Cellular Immunity via the Production of an Adhesion-inhibitory Factor against Immunosurveillance Cells

Ishii

Adachi

Hamamoto

et al. 2014

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Decrease In Silkworm Hemocyte Number By Injury Stimulation Amentioning

confidence: 94%

mentioning

confidence: 91%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Serratia marcescens Suppresses Host Cellular Immunity via the Production of an Adhesion-inhibitory Factor against Immunosurveillance Cells

Ishii

Adachi

Hamamoto

et al. 2014

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

Paralytic Peptide: An Insect Cytokine That Mediates Innate Immunity

Ishii

Hamamoto

Sekimizu

2014

Arch Insect Biochem Physiol

Self Cite

View full text Add to dashboard Cite

Host animals combat invading pathogens by activating various immune responses. Modulation of the immune pathways by cytokines is critical for efficient pathogen elimination. Insects and mammals possess common innate immune systems, and individual immune pathways have been intensively studied over the last two decades. Relatively less attention, however, has been focused on the functions of cytokines in insect innate immunity. Here, we summarize our recent findings from studies of the insect cytokine, paralytic peptide, in the silkworm Bombyx mori. The content of this report was presented at the First Asian Invertebrate Immunity Symposium. Acute activation of paralytic peptide occurs via proteolysis after stimulation with the cell wall components of pathogens, leading to the induction of a wide range of cellular and humoral immune responses. The pathogenic bacterium Serratia marcescens suppresses paralytic peptide-dependent immune activation, which impairs host resistance. Studies of insect cytokines will broaden our understanding of the basic mechanisms underlying the interaction between host innate immunity and pathogenic agents.

show abstract

Serratia marcescens induces apoptosis in Diaphorina citri gut cells via reactive oxygen species‐mediated oxidative stress

Hu,

Zhao,

Zheng

et al. 2023

Pest Management Science

View full text Add to dashboard Cite

BACKGROUNDAsian citrus psyllid, Diaphorina citri, is a notorious pest in citrus industry because it transmits Candidatus Liberibacter asiaticus, which causes an uncurable, devastating disease in citrus worldwide. Serratia marcescens is widely distributed in various environments that exhibits toxic effects to many insects. To develop strategies for enhancing the efficiency of pathogen‐induced host mortality, a better understanding of the toxicity mechanism of S. marcescens on D. citri is critical.RESULTSS. marcescens KH‐001 successfully colonized D. citri gut by feeding artificial diets, resulting in the damage of cells including nucleus, mitochondria, vesicles, and microvilli. Oral ingestion of S. marcescens KH‐001 strongly induced apoptosis in gut cells by enhancing levels of Cyt c, p53 and caspase‐1 and decreasing levels of inhibitors of apoptosis (IAP) and Bax inhibitor‐1 (BI‐1). The expression of dual oxidase (Duox) and nitric oxide synthase (Nos) was up‐regulated by S. marcescens KH‐001, which increased hydrogen peroxide (H2O2) levels in the gut. Injection of abdomen of D. citri with H2O2 accelerated the death of the adults and induced apoptosis in the gut cells by activating Cyt c, p53 and caspase‐1 and suppressing IAP and BI‐1. Pretreatment of infected D. citri with vitamin c (Vc) increased the adult survival and diminished the apoptosis‐inducing effect.CONCLUSIONSThe colonization of S. marcescens KH‐001 in the guts of D. citri increased H2O2 accumulation, leading to severe changes and apoptosis in intestinal cells, which enhanced a higher mortality level of D. citr. This study identifies the underlying virulence mechanism of S. marcescens KH‐001 on D. citri that contributes to a widespread application in the integrated management of citrus psyllid.This article is protected by copyright. All rights reserved.

show abstract

Serratia marcescens Induces Apoptotic Cell Death in Host Immune Cells via a Lipopolysaccharide- and Flagella-dependent Mechanism

Cited by 45 publications

References 57 publications

Serratia marcescens Suppresses Host Cellular Immunity via the Production of an Adhesion-inhibitory Factor against Immunosurveillance Cells

Serratia marcescens Suppresses Host Cellular Immunity via the Production of an Adhesion-inhibitory Factor against Immunosurveillance Cells

Paralytic Peptide: An Insect Cytokine That Mediates Innate Immunity

Serratia marcescens induces apoptosis in Diaphorina citri gut cells via reactive oxygen species‐mediated oxidative stress

Contact Info

Product

Resources

About