Serpulina hyodysenteriae challenge of fattening pigs vaccinated with an adjuvanted bivalent bacterin against swine dysentery

Diego, Rafael Gómez de; Lanza, I; Carvajal, Ana; Rubio, Pedro; Cármenes, Pedro

doi:10.1016/0264-410x(94)00018-i

Cited by 21 publications

(14 citation statements)

References 15 publications

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“…However, attempts to date have met with limited success. Tested vaccines have included whole-cell bacterins [106,107,108,109] and orally administered attenuated strains [34,110,111,112]. Bacterin vaccines may provide some level of protection but they do not provide adequate cross-protective immunity against strains of different serogroups, which would require the use of autogenous or multivalent bacterins.…”

Section: The Fight Against Sdmentioning

confidence: 99%

Swine Dysentery: Aetiology, Pathogenicity, Determinants of Transmission and the Fight against the Disease

Álvarez‐Ordóñez

Martínez-Lobo

Argüello

et al. 2013

IJERPH

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Swine Dysentery (SD) is a severe mucohaemorhagic enteric disease of pigs caused by Brachyspira hyodysenteriae, which has a large impact on pig production and causes important losses due to mortality and sub-optimal performance. Although B. hyodysenteriae has been traditionally considered a pathogen mainly transmitted by direct contact, through the introduction of subclinically infected animals into a previously uninfected herd, recent findings position B. hyodysenteriae as a potential threat for indirect transmission between farms. This article summarizes the knowledge available on the etiological agent of SD and its virulence traits, and reviews the determinants of SD transmission. The between-herds and within-herd transmission routes are addressed. The factors affecting disease transmission are thoroughly discussed, i.e., environmental survival of the pathogen, husbandry factors (production system, production stage, farm management), role of vectors, diet influence and interaction of the microorganism with gut microbiota. Finally, prophylactic and therapeutic approaches to fight against the disease are briefly described.

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Section: The Fight Against Sdmentioning

confidence: 99%

Swine Dysentery: Aetiology, Pathogenicity, Determinants of Transmission and the Fight against the Disease

Álvarez‐Ordóñez

Martínez-Lobo

Argüello

et al. 2013

IJERPH

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“…Previous vaccination trials using killed or attenuated live cells as vaccines have not stimulated immunoprotection against swine dysentery (15,16), while commercially available bacterin vaccines fail to provide complete protection (8). An alternative approach may be to generate subunit vaccines that might be delivered by the expression of recombinant protein on the outer membrane of a bacterial delivery vector, such as Salmonella enterica serovar Typhimurium (9,17).…”

mentioning

confidence: 99%

Differential Expression of the Bhmp39 Major Outer Membrane Proteins of Brachyspira hyodysenteriae

et al. 2006

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The enteric, anaerobic spirochete Brachyspira hyodysenteriae is the causative agent of swine dysentery, a severe mucohemorrhagic diarrheal disease of pigs that has economic significance in every major porkproducing country. Recent investigation into potential vaccine candidates has focused on the outer membrane proteins of B. hyodysenteriae. Bhmp39 (formerly Vsp39) is the most abundant surface-exposed outer membrane protein of B. hyodysenteriae; its predicted gene sequence has previously been shown to share sequence similarity to eight genes divided evenly between two paralogous loci. The peptide sequence suggested that Bhmp39 is encoded by one of these genes, bhmp39h. The biological significance of maintaining eight homologous bhmp39 genes is unclear, though it has been proposed that this may play a role in antigenic variation. In this study, real-time, reverse transcription-PCR was used to demonstrate that bhmp39f and bhmp39h were the transcripts most abundantly expressed by B. hyodysenteriae strain B204 cultured under in vitro growth conditions. Mass spectrometry data of the purified 39-kDa membrane protein showed that both Bhmp39f and Bhmp39h were present. Northern blot analysis across predicted Rho-independent terminators demonstrated that the genes of the bhmp39efgh locus result in monocistronic transcripts.

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“…Previously, bacterin vaccines against B. hyodysenteriae have been reported to offer variable and generally incomplete protection against swine dysentery in pigs ( Fernie et al, 1983, Hampson et al, 1993, Diego et al, 1995and Waters et al, 1999, whilst a bacterin against B. pilosicoli did not prevent colonization with this spirochaete in experimentally infected pigs (Hampson et al, 2000). Hence the failure of the current bacterin to offer protection in laying chickens is not altogether surprising.…”

Section: Discussionmentioning

confidence: 98%

Vaccination with an autogenous bacterin fails to prevent colonization by Brachyspira intermedia in experimentally infected laying chickens

Amin¹,

Phillips²,

La³

et al. 2009

Veterinary Microbiology

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Avian intestinal spirochaetosis (AIS) is a disease complex affecting adult laying and breeding chickens associated with infection by anaerobic intestinal spirochaetes of the genus Brachyspira.Options for control of AIS are limited, as few effective antimicrobial agents are registered for use in laying chickens. One of the two most commonly encountered pathogenic species in AIS is B.intermedia, and the aim of the current study was to determine whether a B. intermedia bacterin vaccine would help control AIS caused by this species. An autogenous bacterin was prepared from B.intermedia strain HB60 and given twice intramuscularly at a 3-week interval to 12 laying chickens housed in individual cages. Twelve non-vaccinated control chickens were placed in adjacent cages in the same room. Two weeks after the second vaccination all the chickens were experimentally challenged with B. intermedia HB60 by crop tube. Subsequently faeces were cultured for spirochaetes every 2-3 days, faecal water content and chicken weight were measured weekly, and egg numbers and weights were recorded daily. Serum was taken prior to both vaccinations, at the time of challenge and at euthanasia. The chickens were killed 6 weeks post-challenge. The vaccinated chickens showed seroconversion to the vaccine, but antibody levels declined significantly post-infection. In comparison, the non-vaccinated chickens showed seroconversion post-infection. The reason for the reduction in the antibody levels in the vaccinated chickens after infection was not explained. At some point all the chickens excreted spirochaetes in their faeces, and the duration of excretion was not different between vaccinated and non-vaccinated chickens. There were no differences in faecal water content, chicken weights, egg production, or gross and microscopic caecal lesions between vaccinated

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Serpulina hyodysenteriae challenge of fattening pigs vaccinated with an adjuvanted bivalent bacterin against swine dysentery

Cited by 21 publications

References 15 publications

Swine Dysentery: Aetiology, Pathogenicity, Determinants of Transmission and the Fight against the Disease

Swine Dysentery: Aetiology, Pathogenicity, Determinants of Transmission and the Fight against the Disease

Differential Expression of the Bhmp39 Major Outer Membrane Proteins of Brachyspira hyodysenteriae

Vaccination with an autogenous bacterin fails to prevent colonization by Brachyspira intermedia in experimentally infected laying chickens

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