Serpins in Hemostasis as Therapeutic Targets for Bleeding or Thrombotic Disorders

Bianchini, Elsa P.; Auditeau, Claire; Razanakolona, Mahita; Vasse, Marc; Borgel, Delphine

doi:10.3389/fcvm.2020.622778

Cited by 11 publications

(8 citation statements)

References 52 publications

(59 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An increased expression in injured tissues of swine 2 and swine 3 after EMF application suggests the beneficial role of EMF in promoting oligodendrocyte proliferation and myelination, both required for the repair of the injured tissue. SERPINE 1 (serine proteinase inhibitor (serpin) family E member 1) encoding for protein plasminogen activator inhibitor 1 (PAI-1) is involved in hemostasis (normal blood clotting) after an injury as a powerful procoagulant by inhibiting tPA/uPA, thrombomodulin, and activated protein C [ 19 , 20 ]. PAI-1 also regulates immune cell infiltration [ 21 ].…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic Analysis of Gene Expression and Effect of Electromagnetic Field in Brain Tissue after Traumatic Brain Injury

Rai,

Mendoza-Mari,

Brazdzionis

et al. 2024

J Biotechnol Biomed

View full text Add to dashboard Cite

Traumatic brain injury (TBI) due to a direct blow or penetrating injury to the head damages the brain tissue and affects brain function. Primary and secondary damage to the brain tissue increases disability, morbidity, and mortality and costs millions of dollars in treatment. Injury to the brain tissue results in the activation of various inflammatory and repair pathways involving many cellular and molecular factors. Increased infiltration of immune cells to clear the debris and lesion healing, activation of Schwann cells, myelination, oligodendrocyte formation, and axonal regeneration occur after TBI to regenerate the tissue. However, secondary damage to brain tissue results in behavioral symptoms. Repair and regeneration are regulated by a complex cascade involving various cells, hormones, and proteins. A change in the expression of various proteins due to altered gene expression may be the cause of impaired repair and the sequelae in TBI. In this pilot study, we used a Yucatan miniswine model of TBI with and without electromagnetic field (EMF) stimulation and investigated the differential gene expression between injured and non-injured cortex tissues. We found several differentially expressed genes including INSC, TTR, CFAP126, SEMA3F, CALB1, CDH19, and SERPINE1. These genes are associated with immune cell infiltration, myelination, reactive oxygen species regulation, thyroid hormone transportation, cell proliferation, and cell migration. There was a time-dependent effect of EMF stimulation on the gene and protein expression. The findings support the beneficial effect of EMF stimulation in the repair process following TBI.

show abstract

Section: Resultsmentioning

confidence: 99%

Transcriptomic Analysis of Gene Expression and Effect of Electromagnetic Field in Brain Tissue after Traumatic Brain Injury

Rai,

Mendoza-Mari,

Brazdzionis

et al. 2024

J Biotechnol Biomed

View full text Add to dashboard Cite

show abstract

“…Sometimes the homeostatic process is not able to recover, and a pathological condition is generated. For this reason, in the literature, many papers that try to explain this mechanism have been published; however, for historical and cultural reasons, most of these articles are clinical and therefore explain how homeostasis is implemented at the cellular level (see, e.g., [35][36][37]). On the other hand, in the last twenty years, there has also been an increasing number of papers that try to describe the mechanism through mathematical models (see, among others, [32,[38][39][40][62][63][64]).…”

Section: Discussionmentioning

confidence: 99%

“…The proposed model is composed of a set of two nonlinear ordinary differential equations (ODEs), where each term of the equations has a well-defined function in the feedback mechanism underlying the physiological process and a precise mathematical characterisation. It is well-known that many papers, dealing with the modelling of physiological systems, have been published in the literature [32,[35][36][37][38][39][40]. However, they have some drawbacks, such as, for example, to investigate only the anatomical issues; hence, they can only describe specific cases.…”

Section: Introductionmentioning

confidence: 99%

A General Approach for the Modelling of Negative Feedback Physiological Control Systems

et al. 2023

View full text Add to dashboard Cite

Mathematical models can improve the understanding of physiological systems behaviour, which is a fundamental topic in the bioengineering field. Having a reliable model enables researchers to carry out in silico experiments, which require less time and resources compared to their in vivo and in vitro counterparts. This work’s objective is to capture the characteristics that a nonlinear dynamical mathematical model should exhibit, in order to describe physiological control systems at different scales. The similarities among various negative feedback physiological systems have been investigated and a unique general framework to describe them has been proposed. Within such a framework, both the existence and stability of equilibrium points are investigated. The model here introduced is based on a closed-loop topology, on which the homeostatic process is based. Finally, to validate the model, three paradigmatic examples of physiological control systems are illustrated and discussed: the ultrasensitivity mechanism for achieving homeostasis in biomolecular circuits, the blood glucose regulation, and the neuromuscular reflex arc (also referred to as muscle stretch reflex). The results show that, by a suitable choice of the modelling functions, the dynamic evolution of the systems under study can be described through the proposed general nonlinear model. Furthermore, the analysis of the equilibrium points and dynamics of the above-mentioned systems are consistent with the literature.

show abstract

“…Many serpin E2 functions are related to its inhibitory effect on plasminogen activator (PA), including regulation of hemostasis, cell adhesion, and promotion of tumor metastasis ( Czekay and Loskutoff, 2009 ; Bharadwaj et al, 2021 ; Bianchini et al, 2021 ). There are two types of PA in humans: uPA and tPA ( Danø et al, 1985 ; Vassalli et al, 1991 ).…”

Section: Serpin E2 Expression and Structurementioning

confidence: 99%

Serpin peptidase inhibitor, clade E, member 2 in physiology and pathology: recent advancements

Wu,

Yang,

Zhang

et al. 2024

Front. Mol. Biosci.

View full text Add to dashboard Cite

Serine protease inhibitors (serpins) are the most numerous and widespread multifunctional protease inhibitor superfamily and are expressed by all eukaryotes. Serpin E2 (serpin peptidase inhibitor, clade E, member 2), a member of the serine protease inhibitor superfamily is a potent endogenous thrombin inhibitor, mainly found in the extracellular matrix and platelets, and expressed in numerous organs and secreted by many cell types. The multiple functions of serpin E2 are mainly mediated through regulating urokinase-type plasminogen activator (uPA, also known as PLAU), tissue-type plasminogen activator (tPA, also known as PLAT), and matrix metalloproteinase activity, and include hemostasis, cell adhesion, and promotion of tumor metastasis. The importance serpin E2 is clear from its involvement in numerous physiological and pathological processes. In this review, we summarize the structural characteristics of the Serpin E2 gene and protein, as well as its roles physiology and disease.

show abstract

Serpins in Hemostasis as Therapeutic Targets for Bleeding or Thrombotic Disorders

Cited by 11 publications

References 52 publications

Transcriptomic Analysis of Gene Expression and Effect of Electromagnetic Field in Brain Tissue after Traumatic Brain Injury

Transcriptomic Analysis of Gene Expression and Effect of Electromagnetic Field in Brain Tissue after Traumatic Brain Injury

A General Approach for the Modelling of Negative Feedback Physiological Control Systems

Serpin peptidase inhibitor, clade E, member 2 in physiology and pathology: recent advancements

Contact Info

Product

Resources

About