Serpentine release by suspension cultures of Catharanthus roseus

Goodbody, Anne E.; Lambe, C. A.; Rosevear, A.

doi:10.1007/bf01033200

Cited by 7 publications

(1 citation statement)

References 8 publications

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“…In these studies, the accumulation of alkaloids was more pronounced with 21 day old cells as compared to 7 day old cells. Similarly, C. roseus cells in suspension cultures released serpentine when transferred to fresh medium (31). In contrast, the highest increase of rosmarinic acid content was obtained when 6 day old cell cultures of Lithospermum erythrorhizon were treated with elicitor (32).…”

Section: Resultsmentioning

confidence: 99%

Influence of Fungal Elicitors on Production of Ajmalicine by Cell Cultures of Catharanthus roseus

Namdeo¹,

Patil

Fulzele

2002

Biotechnology Progress

119

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Suspension cultures of Catharanthus roseus (C. roseus) were elicited with fungal cell wall fragments of Aspergillus niger (A. niger), Fusarium moniliforme (F. moniliforme), and Trichoderma viride (T. viride). The effects of elicitor dosage, exposures time, and age of subculture on ajmalicine accumulation were studied. A higher concentration of elicitor extract responded positively to C. roseus suspension cultures. Ajmalicine accumulation increased by about 3-fold when cells were treated with A. niger, F.moniliforme, and T. viride. The maximum ajmalicine production (75 microg g(-1) dry weight (DW)) was observed in cells treated with T. viride. Cell cultures were elicited with 5% preparation of A. niger, F. moniliforme, and T. viride and exposed for 24, 48, 72, and 96 h. for elicitation. Suspension cultures elicited with T. viride for 48 h showed a 3-fold increase (87 microg g(-1) DW) in ajmalicine contents, whereas A. niger and F. moniliforme synthesized a 2-fold increase in alkaloid and yielded 52 and 56 microg g(-1) DW ajmalicine, respectively. C. roseus cells of different age (5,10, 15, 20, and 25 days old) were treated with a 5% elicitor of A. niger, F. moniliforme, and T. viride and investigated elicitors activity at different age of cell cultures. Maximum yield 166 microg g(-1) DW of ajmalicine was synthesized in 20 day old suspension cultures treated with T. viride. A longer period of incubation of cell cultures with elicitors adversely affected the ajmalicine synthesis.

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Section: Resultsmentioning

confidence: 99%

Influence of Fungal Elicitors on Production of Ajmalicine by Cell Cultures of Catharanthus roseus

Namdeo¹,

Patil

Fulzele

2002

Biotechnology Progress

119

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Catharanthus Species

2020

Secondary Metabolites of Medicinal Plants

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Production of indole alkaloids by surface immobilized C. roseus cells

Archambault

Volesky

Kurz

1990

Biotech & Bioengineering

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A technique was developed to surface immobilize plant cells and was scaled up in laboratory size bioreactors. This technique was shown not to hinder the biosynthetic potential of Catharanthus roseus immobilized cells and to induce a partial release (300 microg/L) of serpentine into the culture medium contrary to suspension cultured cells. The release pattern seemed to follow the biosynthesis trends of the product. This release mechanism could be stimulated by a factor of 10 within 2 h by increasing the pH of the culture from 5.0 to 5.5.

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Serpentine release by suspension cultures of Catharanthus roseus

Cited by 7 publications

References 8 publications

Influence of Fungal Elicitors on Production of Ajmalicine by Cell Cultures of Catharanthus roseus

Influence of Fungal Elicitors on Production of Ajmalicine by Cell Cultures of Catharanthus roseus

Catharanthus Species

Production of indole alkaloids by surface immobilized C. roseus cells

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