Serpentine ecotypic differentiation in a polyploid plant complex: shared tolerance to Mg and Ni stress among di- and tetraploid serpentine populations of Knautia arvensis (Dipsacaceae)

Kolář, Filip; Dortová, Markéta; Lepš, Jan; Pouzar, Miloslav; Krejčová, Anna; Štech, Milan

doi:10.1007/s11104-013-1813-y

Cited by 16 publications

(24 citation statements)

References 38 publications

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“…Finally, our design also does not allow us to pinpoint the particular edaphic factor responsible for the differential ploidy response to the substrate. As K. serpentinicola does not exhibit any obvious ploidy‐driven response to serpentine toxicity (Kolář et al ., ), we consider differential concentrations of key nutrients (such as P, K and Ca that are markedly elevated in the nonserpentine substrate) as the most likely explanation.…”

Section: Discussionmentioning

confidence: 99%

“…Previous work has shown that both cytotypes of K . serpentinicola are well adapted to serpentine challenge via their physiology (Kolář et al ., ) and symbiosis with arbuscular mycorrhizal fungi (Doubková et al ., ). Although hydroponic cultivation has shown a similar response of both cytotypes to serpentine chemistry at the level of seedlings (manipulated Ca 2+ /Mg 2+ and Ni + , Kolář et al ., ), the performance of adult plants in their native serpentine substrate was unknown.…”

Section: Methodsmentioning

confidence: 99%

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Ploidy‐altered phenotype interacts with local environment and may enhance polyploid establishment inKnautia serpentinicola(Caprifoliaceae)

et al. 2018

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Whole genome duplication is a key process in plant evolution and has direct phenotypic consequences. However, it remains unclear whether ploidy-related phenotypic changes can significantly alter the fitness of polyploids in nature and thus contribute to establishment of new polyploid mutants in diploid populations. We addressed this question using a unique natural system encompassing a diploid and its sympatric locally established autotetraploid derivative. By setting a common garden experiment with two manipulated environmental factors (presence/absence of serpentine substrate and competition), we tested whether these two locally important factors differently shape the phenotypic response of the two ploidy levels. Tetraploids attained significantly higher values of both above- and below-ground biomass, and root : shoot ratio compared to their diploid progenitors. Tetraploid superiority in vegetative fitness indicators was most prominent when they were cultivated together with a competitor in nutrient-rich nonserpentine substrate. We show that even genetically very closely related diploids and tetraploids can respond differently to key environmental factors. Provided there are sufficient nutrients, tetraploids can be more successful in tolerating interspecific competition than their diploid progenitors. Such superior performance might have provided an adaptive advantage for the newly established tetraploid promoting colonisation of new (micro-)habitats, which was indeed observed at the natural site.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Ploidy‐altered phenotype interacts with local environment and may enhance polyploid establishment inKnautia serpentinicola(Caprifoliaceae)

et al. 2018

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“…Quintela-Sabarís et al (2010) and Kolář et al (2014b) report no evidence of genetic differentiation between serpentinite and non-serpentinite populations of target vascular plants. Kolář et al (2014a), by contrast, report Mg stress as an adaptive character of Knautia arvensis. The reasons for these differences may be the different scales at which the studies were carried out, different physiological effect of magnesium on individual species and the effect of other factors that mask the magnesium effect, such as geography or drought.…”

Section: Magnesium Toxicity As An Evolutionary Trigger For Wetland Brmentioning

confidence: 84%

“…Magnesium-rich environments generally act as an important evolutionary trigger for vascular plants and ferns (Rajakaruna 2004, Kolář et al 2012, 2014a, Vicić et al 2014, but for bryophytes the evidence for serpentinite or dolomite ecotypes is poor (Shaw 1991). We aimed to advance the understanding of the genetic background of calciumtolerance in bryophytes by answering the following questions: (i) Will the significant genetic variation in S. warnstorfii reported by Mikulášková et al (2015) be confirmed using a new independent data set?…”

Section: Introductionmentioning

confidence: 97%

Microsatellite variation in three calcium-tolerant species of peat moss detected specific genotypes of Sphagnum warnstorfii on magnesium-rich bedrock

Mikulášková¹,

Veleba²,

Šmerda³

et al. 2017

Preslia

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Peat mosses are a key functional group in peatlands, driving biogeochemical cycles, habitat development and changes in species composition. They are generally intolerant of calcium and magnesium bicarbonate, but some species are adapted to mineral-rich fens. A previous study found a coincidence between genetic variation and the ability to tolerate high pH/calcium levels in Sphagnum warnstorfii. Here we compare its microsatellite variation with that of two rarer calcium-tolerant species (Sphagnum subnitens, S. contortum), using a novel data set from Eurasia. Because physiological experiments indicate that S. warnstorfii can tolerate high magnesium levels, we included also samples from dolomite and serpentinite. Genetic diversity of S. warnstorfii was higher than that of other species. The Bayesian analysis in program Structure resulted in two population groups of S. warnstorfii. One group coincided with dolomite (Italy, Austria, Estonia) and moderately magnesium-rich (but calcium-poor) rocks (serpentinite, metadolerite, cordieritebearing migmatite on the Bohemian Massif), while the second one coincided with magnesiumpoor bedrock across Eurasia. The principal coordinate analysis revealed a cline between populations from magnesium-rich and magnesium-poor bedrocks, with populations from dolomite and serpentinite forming one extreme. Populations from magnesium-poor bedrock located far from any dolomite or serpentinite formed the opposite extreme of the cline. We demonstrate for the first time that magnesium toxicity may drive bryophyte microevolution, as has repeatedly been shown for vascular plants, including ferns. K e y w o r d s: Bryophyta,

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“…Their serpentine counterparts occupy stable communities such as open forests and rocky grasslands (e.g. Š těpánek 1997) and exhibit tolerance to extreme chemical properties of serpentine soil (Kolář et al 2014). K. arvensis has been reported to possess the ability to resprout from roots after injury on arable land (Wehsarg 1954), but no information exists on how this ability varies in different soil lines or ploidy levels.…”

Section: Introductionmentioning

confidence: 99%

Root sprouting in Knautia arvensis (Dipsacaceae): effects of polyploidy, soil origin and nutrient availability

et al. 2015

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Plants able to resprout from roots have a potential bud bank that gets initiated after injury to overcome meristem limitation after loss of all stem parts and to facilitate regeneration. Knautia arvensis is reportedly able to sprout from its roots on arable land, but information is missing regarding such ability in serpentine populations or how it might differ between diploids and tetraploids. We hypothesized that (1) 'ruderal' non-serpentine populations better tolerate severe disturbance than relic, serpentine ones; (2) tetraploid populations resprout more readily than diploids due to enhanced growth of higher ploidy levels; and (3) plants of different ploidy levels from serpentine soils are, for evolutionary reasons, more similar in their response to disturbances than plants from non-serpentine soils. To test these hypotheses, we conducted a pot experiment. Its results do not support our hypothesis that the ability to sprout from roots is a factor driving the spread of new weedy taxa into central Europe or the hypothesis that it is related to polyploidization in the genus Knautia. Both tetraploids and plants from non-serpentine populations regenerated less vigorously than diploids and plants from serpentine populations. However, the genetically closer populations of serpentine origin were more similar in their response to experimental manipulations than their genetically distinct non-serpentine counterparts. The success of non-serpentine taxa in disturbed habitats of central Europe might be related to traits other than the ability to resprout.

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Serpentine ecotypic differentiation in a polyploid plant complex: shared tolerance to Mg and Ni stress among di- and tetraploid serpentine populations of Knautia arvensis (Dipsacaceae)

Cited by 16 publications

References 38 publications

Ploidy‐altered phenotype interacts with local environment and may enhance polyploid establishment inKnautia serpentinicola(Caprifoliaceae)

Ploidy‐altered phenotype interacts with local environment and may enhance polyploid establishment inKnautia serpentinicola(Caprifoliaceae)

Microsatellite variation in three calcium-tolerant species of peat moss detected specific genotypes of Sphagnum warnstorfii on magnesium-rich bedrock

Root sprouting in Knautia arvensis (Dipsacaceae): effects of polyploidy, soil origin and nutrient availability

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