Serovar determination, drug resistance patterns and plasmid profiles of Pseudomonas aeruginosa isolated from burn patients at two hospitals of Tehran (IRAN)

Shahcheraghi, F.; Feizabadi, Mehdi; Yamin, V; Abiri, Ramin; Abedian, Z

doi:10.1016/s0305-4179(03)00142-6

Cited by 24 publications

(26 citation statements)

References 16 publications

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“…Drug susceptibility: Multidrug-Resistant (MDR) bacteria have commonly been reported as the cause of nosocomial outbreaks of infection in burn units or as colonizers of the wounds of burn patients [1,14,15,19,20] . Antimicrobial resistance has been reached to a troubling point in P. aeruginosa isolated from burn patients in Iran.…”

Section: Discussionmentioning

confidence: 99%

“…RAPD-PCR mixtures were set up and incubated as previously described [16] . Reactions mixtures (25 µL) were made optimum for P. aeruginosa and contained 40 ng of genomic DNA, 40 p mol of primer 272 (MWG Biotech, Germany, AGCGGGCCAA) [19] , 1 unit of Taq DNA polymerase (MBI Fermentas, Vilnius, Lithuania), 250 mM of each deoxynucleoside triphosphate (MBI Fermentas), 1x reaction buffer supplied by manufacturer and 3 mM MgCl 2 (MBI Fermentas). DNA was amplified in ASTEC PCR Thermal Cycler PC 707-02 (ASTEC, Fukuoka, Japan) with the cycling conditions as follows: (i) 94°C for 5 min, 36°C for 5 min and 72°C 5 min, for 4 cycles and (ii) 94°C for 1 min, 36°C for 1 min and 72°C for 2 min, for 30 cycles, followed by a final extension step at 72°C for 10 min.…”

Section: Rapd Analysismentioning

confidence: 99%

“…P. aeruginosa is a gram-negative human opportunistic pathogen that infects burn patients with immunological system defects and causes a wide range of infections. Since this bacterium is naturally resistant to many drugs and is able to get resistance to all effective antibiotics, the infection with this organism is particularly problematic condition for patients [6,8,14,15,19] . Multidrug-Resistant (MDR) bacteria have commonly been reported as the cause of nosocomial outbreaks of infection in burn units or as colonizers of the wounds of burn patients.…”

Section: Introductionmentioning

confidence: 99%

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Drug Susceptibility and Molecular Epidemiology of Pseudomonas aeruginosa Isolated in a Burn Unit

Salimi¹,

Owlia²,

Yakhchali³

et al. 2009

American J. of Infectious Diseases

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Problem statement:Pseudomonas aeruginosa is an opportunistic pathogen that still develops life-threatening infections in patients with immunological system defects, like burn patients, especially in developing countries. Since this bacterium is naturally resistant to many drugs and is able to get resistance to all effective antibiotics, the infection with this organism is particularly problematic condition for patients. This study was design to evaluated drug susceptibility for determination of multidrug-resistant isolates and molecular epidemiology of P. aeruginosa colonization for investigation of the isolates routes in the burn unit of Shahid Motahhari Hospital in Tehran. Approach: About 127 clinical and 2 environmental P. aeruginosa isolates were collected during 6 months. All P. aeruginosa isolates were analyzed for drug susceptibility by disk diffusion method and molecular epidemiology assessment were done by RAPD-PCR analysis. Results: Drug susceptibility tests were shown high resistance for ceftizoxime (86.8%), aztreonam (80.6%), kanamycin (79.8%) and tetracycline (78.3%); furthermore, low resistance for some antibiotics like imipenem (30.2%), piperacillin/tazobactam (34.1%) and amikacin (41.1%) was showed. In addition, 42 multidrug-resistant P. aeruginosa isolates were recovered from clinical specimens and one isolate from environmental samples. Molecular typing revealed eight different profiles that include two profiles, RAPD1 and RAPD4, with environmental resource. The major RAPD profile was RAPD1 profile (n = 64, 50.4%), which includes 31 (72.1%) multidrug-resistant isolates with an environmental reservoir. Conclusion: In summery, we were found three different profiles for multidrug-resistant strains. Different RAPD profiles suggested the different resources of infection, two environmental resources were found, that one of them was multidrug-resistant strain. These findings highlighted the need for further attention to disinfection inanimate hospital environment and controlled contact between staff with patients to limit transfer of P. aeruginosa in this BU; moreover, use of some antimicrobial agents must be restricted due to existence of high resistance and using of combined effective antibiotics is recommend.

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Section: Discussionmentioning

confidence: 99%

Section: Rapd Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Drug Susceptibility and Molecular Epidemiology of Pseudomonas aeruginosa Isolated in a Burn Unit

Salimi¹,

Owlia²,

Yakhchali³

et al. 2009

American J. of Infectious Diseases

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“…Plasmid DNA for electroporation was prepared using a Wizard Plus mini-prep kit (Promega). Plasmid DNA for profiling was prepared using a modification of published methods (21,43). Cells from overnight growth on horse blood agar plates were suspended in 50 l of TE buffer (10 mM Tris-HCl, 1 mM EDTA [pH 8.5]), and 200 l of lysis solution (3% [wt/vol] sodium dodecyl sulfate, 50 mM Tris [pH 12.5]) was added.…”

Section: Methodsmentioning

confidence: 99%

Horizontal Gene Transfer in a Polyclonal Outbreak of Carbapenem-ResistantAcinetobacter baumannii

et al. 2007

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In the last few years, phenotypically carbapenem resistant Acinetobacter strains have been identified throughout the world, including in many of the hospitals and intensive care units (ICUs) of Australia. Genotyping of Australian ICU outbreak-associated isolates by pulsed-field gel electrophoresis of whole genomic DNA indicated that different strains were cocirculating within one hospital. The carbapenem-resistant phenotype of these and other Australian isolates was found to be due to carbapenem-hydrolyzing activity associated with the presence of the bla OXA-23 gene. In all resistant strains examined, the bla OXA-23 gene was adjacent to the insertion sequence ISAba1 in a structure that has been found in Acinetobacter baumannii strains of a similar phenotype from around the world; bla OXA-51 -like genes were also found in all A. baumannii strains but were not consistently associated with ISAba1, which is believed to provide the promoter required for expression of linked antibiotic resistance genes. Most isolates were also found to contain additional antibiotic resistance genes within the cassette arrays of class 1 integrons. The same cassette arrays, in addition to the ISAba1-bla OXA-23 structure, were found within unrelated strains, but no common plasmid carrying these accessory genetic elements could be identified. It therefore appears that antibiotic resistance genes are readily exchanged between cocirculating strains in epidemics of phenotypically indistinguishable organisms. Epidemiological investigation of major outbreaks should include whole-genome typing as well as analysis of potentially transmissible resistance genes and their vehicles.Acinetobacter spp. are emerging opportunistic nosocomial pathogens, with increasing prevalence worldwide. Their epidemiology is complex, and genotypic methods or a combination of genotypic and phenotypic methods are required for species identification (28). Differentiation of Acinetobacter baumannii and Acinetobacter genomic species 3 and 13TU (the three most clinically important members of the genus) and the environmental species A. calcoaceticus is impractical in the routine laboratory, and these four species are generally grouped phenotypically as the A. calcoaceticus-A. baumannii complex. DNA fingerprinting by ApaI digestion of total DNA and pulsed-field gel electrophoresis (PFGE) is regarded as the typing method of choice for outbreak investigation and can be used to compare outbreaks in different locations when the methodology is uniform (41).Studies at the species level have shown that the problems of epidemic spread of antibiotic-resistant Acinetobacter strains are mostly due to bacteria belonging to the A. calcoaceticus-A. baumannii complex (3). The potential for acquisition of transferable carbapenem resistance has long been recognized (37a), and the adjusted mortality risk for intensive-care patients infected by carbapenem resistant Acinetobacter may be increased more than threefold (33).Carbapenem resistance in strains of the A. calcoaceticus-A.baumannii comp...

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“…In previous studies, gentamicin resistance rate from 63% up to 93.7%, amikacin from 25% up to 93.4%, I mipenem from 14% up to 50% was reported (Shahcheraghi et al, 2003,Kianpouret al, 2010,Anil and Shahid,2013,Nikokar et al, 2013,Chaudhar et al, 2013. In this study, ceftazidime and cefepime showed resistance rate of 77.4% and 75.4%; respectively.…”

Section: Resultsmentioning

confidence: 90%

Molecular Study of Efflux Genes in Pseudomonas aeruginosa Isolated from Clinical Samples

Zaki¹,

Elewa²,

Kasaby³

2017

Int.J.Curr.Microbiol.App.Sci

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Serovar determination, drug resistance patterns and plasmid profiles of Pseudomonas aeruginosa isolated from burn patients at two hospitals of Tehran (IRAN)

Cited by 24 publications

References 16 publications

Drug Susceptibility and Molecular Epidemiology of Pseudomonas aeruginosa Isolated in a Burn Unit

Drug Susceptibility and Molecular Epidemiology of Pseudomonas aeruginosa Isolated in a Burn Unit

Horizontal Gene Transfer in a Polyclonal Outbreak of Carbapenem-ResistantAcinetobacter baumannii

Molecular Study of Efflux Genes in Pseudomonas aeruginosa Isolated from Clinical Samples

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