Serotyping of Brunei pneumococcal clinical strains and the investigation of their capability to adhere and invade a brain endothelium model

Rahman, Nurul Adhwa; Sharudin, Aishah; Diah, Suwarni; Muharram, Siti Hanna

doi:10.1016/j.micpath.2017.07.021

Cited by 2 publications

(1 citation statement)

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“…Streptococcus pneumoniae ATCC 49619 was used as the control strain. All of the strains in this study carried the same serotype 19F (Rahman et al 2017).…”

Section: Bacterial Strains Used In This Studymentioning

confidence: 99%

Streptococcus pneumoniae Infection Increases Expression of von Willebrand Factor (vWF) in a Mouse Brain Endothelium Model

Rahman¹

2018

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Faktor von Willebrand (vWF) adalah glikoprotein multimerik besar yang dikeluarkan secara normal oleh sel-sel endotelial otak dan ianya wujud sebagai protein beredar di dalam darah tetapi struktur asal dan matang vWF tidak terbahagi. Jangkitan pneumokokus boleh menyebabkan pembelahan protein, sehingga membentuk beberapa multimer dengan saiz yang berbeza. Kajian ini telah menguji hipotesis adakah jangkamasa jangkitan Streptococcus pneumoniae boleh menentukan pembentukan saiz vWF. Sel-sel endotelial otak tikus (bEnd.3) telah dijangkiti pada waktu tertentu dengan Streptococcus pneumoniae (serotype 19F). Mikroskopi immunofluoresensi telah dilakukan dan dianalisa dengan sewajarnya. Sel-sel juga diekstrak dan kandungan protein telah dipisahkan ke dalam saiz yang berbeza dengan menggunakan Western blot. Ekspresi vWF kemudian dianalisa dan dibandingkan antara sampel dan antara jangkamasa jangkitan tertentu. Pengeluaran vWF adalah didapati meningkat semasa jangkitan. Walaupun pelbagai perkara tidak spesifik boleh menyebabkan pembahagian vWF, kajian ini menunjukkan bahawa bentuk pembahagian vWF adalah berkemungkinan bergantung pada jangkamasa jangkitan bakteria ini.

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“…Streptococcus pneumoniae ATCC 49619 was used as the control strain. All of the strains in this study carried the same serotype 19F (Rahman et al 2017).…”

Section: Bacterial Strains Used In This Studymentioning

confidence: 99%

Streptococcus pneumoniae Infection Increases Expression of von Willebrand Factor (vWF) in a Mouse Brain Endothelium Model

Rahman¹

2018

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In Vitro Blood-Brain Barrier Models for Neuroinfectious Diseases: A Narrative Review

Badawi,

Mohamad,

Stanslas

et al. 2024

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The blood-brain barrier (BBB) is a complex, dynamic, and adaptable barrier between the peripheral blood system and the central nervous system. While this barrier protects the brain and spinal cord from inflammation and infection, it prevents most drugs from reaching the brain tissue. With the expanding interest in the pathophysiology of BBB, the development of in vitro BBB models has dramatically evolved. However, due to the lack of a standard model, a range of experimental protocols, BBB-phenotype markers, and permeability flux markers was utilized to construct in vitro BBB models. Several neuroinfectious diseases are associated with BBB dysfunction. To conduct neuro infectious disease research effectively, there stems a need to design representative in vitro human BBB models that mimic the BBB's functional and molecular properties. The highest necessity is for an in vitro standardised BBB model that accurately represents all the complexities of an intact brain barrier. Thus, this in-depth review aims to describe the optimization and validation parameters for building BBB models and to discuss previous research on neuroinfectious diseases that have utilized in vitro BBB models. The findings in this review may serve as a basis for more efficient optimisation, validation, and maintenance of a structurally- and functionally intact BBB model, particularly for future studies on neuroinfectious diseases.

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Serotyping of Brunei pneumococcal clinical strains and the investigation of their capability to adhere and invade a brain endothelium model

Cited by 2 publications

References 50 publications

Streptococcus pneumoniae Infection Increases Expression of von Willebrand Factor (vWF) in a Mouse Brain Endothelium Model

Streptococcus pneumoniae Infection Increases Expression of von Willebrand Factor (vWF) in a Mouse Brain Endothelium Model

In Vitro Blood-Brain Barrier Models for Neuroinfectious Diseases: A Narrative Review

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