Serotyping and RAPD profiles of Salmonella enterica isolates from Mauritius

Abstract

Aims: The genus Salmonella is a common agent of gastroenteritis in Mauritius, generating more cases of the disease during summer than during winter. The aims of this study were to assess the genetic diversity of isolates of Salmonella enterica by RAPD fingerprinting, and to establish the relationship between human and chicken isolates. Methods: Twenty‐six isolates were obtained from hospital laboratories and commercial poultry producers locally. Results: The RAPD profiles, biochemical and serological analyses showed that two of the chicken isolates were mistakenly identified as Salmonella. The genetic diversity of the remaining 24 isolates (five chicken and 19 human), confirmed as Salmonella, was analysed using four arbitrary primers, OPA‐10, OPR‐03, OPI‐06 and OPJ‐09, chosen from an initial set of 10 decamers. Seventy RAPD markers were generated in four individual DNA profiles. Significance and Impact of the Study: Cluster analysis (UPGMA) performed using the NTSYS‐pc V 1.8 computer software, confirmed that some strains of Salmonella isolated from chicken were genetically similar to those isolated from humans. Furthermore, a 1 kbp band amplified using primer OPA‐10 was specific for the Salmonella genus as it was not amplified in any of the control bacteria.

Genetic diversity of the bacterial wilt pathogen Ralstonia solanacearum using a RAPD marker

“…RAPD markers were used because they have the potential to detect polymorphism in the entire genome as compared to other techniques [23]. RAPD markers have been used for strain differentiation [24], identification and other genetic analysis for several bacterial genera during the last two decades [23,[25][26][27][28]. Taken together, the present study was undertaken to assess the potentiality of RAPD as a marker for analysing the diversity of R. solanacearum collected from some fields of some selected locations.…”

Molecular typing of Salmonella from Sergipe, Northeastern Brazil, showing the epidemiological relationship between poultry and human infection

“…PCR-based fingerprinting using RAPD has been used widely for genome identification, and the RAPD technique using up to six primers has been reported to be a good tool for molecular typing, permitting the execution of public health measures necessary to control infection (Chansiripornchai et al, 2000;De Cesare et al, 2001;Khoodoo et al, 2002;Quintaes et al, 2002). During this study, we found that the best amplification conditions for RAPD-PCR were an annealing temperature of 35ºC with 3 µL primer.…”

Genotypic diversity in a localized population of Ralstonia solanacearum as revealed by random amplified polymorphic DNA markers

“…RAPD was used as it has the potential to detect polymorphism in the entire genome as compared to other techniques (Hilton and Penn 1998). RAPD markers have been used for strain differentiation (Mesquita et al 1998), identification and other genetic analysis for several bacterial genera (Akopyanz et al 1992;Mazurier et al 1992;Parent et al 1996;Hilton and Penn 1998;Khoodoo et al 2002).…”