Serotypes of Cryptococcus neoformans isolated from patients prior to and during the AIDS era in Thailand

Sukroongreung, S; Nilakul, C; Ruangsomboon, Onlak; Chuakul, Wongsatit; Eampokalap, Boonchuay

doi:10.1007/bf00436454

Cited by 41 publications

(26 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While VNI and VGI molecular types were equally predominant in the earlier cases of cryptococcosis (40.9 and 54.4%, respectively), VNI was the most predominant type isolated in recent cases of cryptococcosis (84.6%). This changing pattern of cryptococcal isolates has also been documented for serotypes of the C. neoformans from Thailand [24], another Southeast Asian country, prior to and during the AIDS era.…”

Section: Resultsmentioning

confidence: 89%

Determination of molecular types and genetic heterogeneity ofCryptococcus neoformansandC. gattiiin Malaysia

et al. 2006

View full text Add to dashboard Cite

The molecular types and genetic heterogeneity of Cryptococcus neoformans and C. gattii clinical isolates in Malaysia were determined in this study. Of 44 C. neoformans collected between 1980 and 2003, 42 (95.5%) were molecular type VNI, 2 (4.5%) were molecular type VNII. Of 17 C.gattii isolates, 13 (76.5%) were molecular type VGI, and 4 (23.5%) were molecular type VGII. A difference was noted when comparing the molecular types of cryptococcal isolates in the earlier and recent cases of cryptococcosis. While both molecular types VNI and VGI were equally predominant in the earlier cases of cryptococcosis, VNI was the most predominant molecular type isolated from the recent cases. VNII was a new molecular type, isolated from 5.1% of the recent cases. All the bird dropping isolates were molecular type VNI. The genetic heterogeneity of the two predominant molecular types, i.e., VNI, VGI clinical isolates and bird dropping isolates of C. neoformans were further determined by polymerase chain reaction (PCR) fingerprinting method, using (GTG)5 as single primer. Two clusters of cryptococcal isolates were distinguished at 68.5% of similarity, with cluster I consisting of VNI isolates and cluster II consisting of VGI isolates. Each cluster was further subdivided into three subtypes at >/=80% of similarity. Fourteen bird dropping isolates were grouped into a subtype within VN1, sharing 82.7% of similarity with the clinical isolates. A higher degree of similarities, ranging from 93.4-97.6% was noted between 3 bird dropping isolates with the clinical isolates in another subtype. This study demonstrated the existence of various molecular types of C. neoformans isolates in Malaysia and the genetic heterogeneity within the predominant molecular types. The study also provides evidence for genetic relatedness of clinical isolates with bird dropping isolates in the environment.

show abstract

Section: Resultsmentioning

confidence: 89%

Determination of molecular types and genetic heterogeneity ofCryptococcus neoformansandC. gattiiin Malaysia

et al. 2006

View full text Add to dashboard Cite

show abstract

“…It was subsequently demonstrated that the most prevalent serotype in the United States was A (25) and that the dominant serotype in Thailand changed with the emergence of AIDS (378). The usefulness of serotyping C. neoformans has been confirmed by the large number of studies employing the method-more than 150 studies were identified in a MedLine search of the bibliography database in 1998, and that represented an underestimate.…”

Section: Biotyping Is Inadequate As a Dna Fingerprinting Methodsmentioning

confidence: 99%

“…Indeed, a change in the dominant serotype of C. neoformans accompanied the emergence of AIDS in Thailand (378). In addition, there is evidence of rapidly emerging pathogens such as C. glabrata (136) and C. dubliniensis (79,376) and changes in the proportions of dominant strains of C. albicans within a geographical locale over time (D. R. Soll, unpublished observations).…”

Section: Problems Of Collectionmentioning

confidence: 99%

The Ins and Outs of DNA Fingerprinting the Infectious Fungi

Soll

2000

Clinical Microbiology Reviews

283

317

View full text Add to dashboard Cite

“…Most clinical serotype C strains were isolated from immunocompetent patients in southern California [3]; in addition, there are reports of the isolation of serotype C from immunocompetent patients in Canada [3], the United Kingdom [3], Thailand [4], Mexico [5], Brazil [6], and Colombia [7]. On the basis of molecular fingerprinting, all serotype C strains have been associated with either of 2 genetically isolated molecular types, VGIII and VGIV [5].…”

mentioning

confidence: 96%

Prevalence of Clinical Isolates ofCryptococcus gattiiSerotype C among Patients with AIDS in Sub‐Saharan Africa

Litvintseva¹,

et al. 2005

View full text Add to dashboard Cite

Cryptococcus gattii is a group of exogenous, neurotropic yeasts that possess the capsular serotype B or C. Isolates of serotype C are extremely rare and, until recently, were known to infect only immunocompetent individuals. We genotyped 176 isolates of Cryptococcus from patients in sub-Saharan Africa who had AIDS; 22 (13.7%) of 161 isolates from Botswana and 2 (13.3%) of 15 isolates from Malawi were C. gattii serotype C strains. All of these serotype C strains belong to the rare VGIV genotype, possess the MAT alpha mating-type allele, and exhibit little genetic diversity.

show abstract

Serotypes of Cryptococcus neoformans isolated from patients prior to and during the AIDS era in Thailand

Cited by 41 publications

References 21 publications

Determination of molecular types and genetic heterogeneity ofCryptococcus neoformansandC. gattiiin Malaysia

Determination of molecular types and genetic heterogeneity ofCryptococcus neoformansandC. gattiiin Malaysia

The Ins and Outs of DNA Fingerprinting the Infectious Fungi

Prevalence of Clinical Isolates ofCryptococcus gattiiSerotype C among Patients with AIDS in Sub‐Saharan Africa

Contact Info

Product

Resources

About