Serotypes, antibiotic resistance, and virulence genes of <i>Salmonella</i> in children with diarrhea

Yue, Meina; Li, Xiaoyü; Liu, Di; Hu, Xue

doi:10.1002/jcla.23525

Cited by 29 publications

(40 citation statements)

References 18 publications

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“…25 , 27 Extended-spectrum cephalosporins, especially ceftriaxone, have previously been suggested as the drugs of first-line choice for treating children with serious nontyphoidal Salmonella infections. However, the drug resistance rates of ceftriaxone remained at a high level in the past ten years, which was significantly higher than Guangdong 12 and Hangzhou 14 in China. Therefore, the drug resistance of ceftriaxone in southwest China is alarming.…”

Section: Discussionmentioning

confidence: 93%

“…Other studies in China also displayed that Salmonella Typhimurium was the most common serotype. 14 , 26 , 44 , 45 And Salmonella Typhimurium was also the most common serotype in North America and Oceania. 46 However, some other studies showed that Salmonella Enteritidis was the most common serotype in China.…”

Section: Discussionmentioning

confidence: 99%

“… 7 The pathogenicity of Salmonella is mainly related to the virulence factors that it carries, including Salmonella pathogenicity islands (SPIs), virulence plasmids, pili, and enterotoxins. 14 The pathogenic factors of NTS will be released to induce the host to develop a mucosal inflammation response after NTS infections. 15 When the host tries to eliminate the bacteria, it may cause “collateral damage” that destroys the human intestinal microecological balance, resulting in clinical symptoms like diarrhea.…”

Section: Introductionmentioning

confidence: 99%

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Prevalence, Clinical Characteristics and Changes of Antibiotic Resistance in Children with Nontyphoidal Salmonella Infections from 2009–2018 in Chongqing, China

Wu¹,

Luo²,

Shi³

et al. 2021

IDR

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Purpose Nontyphoidal Salmonella (NTS) is a leading bacterial cause for acute gastroenteritis in children. With the increasing use of antibiotics worldwide, antibiotic resistance has become a global problem. However, data on NTS infections and changes in antibiotic resistance among children remain limited in China. We aimed to characterize the prevalence, clinical feature, serotype and the changes of antibiotic resistance of NTS in children in Chongqing. Methods 501 hospitalized children with NTS infections (confirmed by positive NTS culture) in Children’s Hospital of Chongqing Medical University from January 2009 to December 2018 were included. The clinical data and drug sensitivity test results were retrospectively reviewed and analyzed. Antibiotic resistance in NTS infections was compared between 2009–2013 and 2014–2018. Results A total of 501 isolates were detected. Most NTS infections occurred in children under three years old, which mainly occurred between July and October. The numbers of patients with diarrhea, fever, and vomiting were 472 (94.2%), 422 (84.2%) and 146 (29.1%), respectively. Serogroup B (67.5%) was the predominant serogroup isolates. And Salmonella Typhimurium was the most common serotype (79.2%). The study compared the drug resistance of NTS from 2009 to 2013 with that from 2014 to 2018. We found the drug resistance rates of NTS to cefazolin, cefotaxime, ciprofloxacin, levofloxacin and imipenem showed an upward trend. The drug resistance rates of NTS to chloramphenicol, ampicillin, ceftriaxone, cefepime and compound sulfamethoxazole decreased slightly, but still showed high drug resistance rates. And drug resistance rates of NTS to piperacillin/tazobactam and ceftazidime decreased significantly in the last ten years. Multi-drug resistance (MDR) isolates, were detected among 69 cases (13.7%) of 501 children with NTS infections. Conclusion The overall antibiotic resistance rates remained at a high level in Chongqing. Continuous surveillance of antibiotic resistance in NTS and control measures such as avoiding unnecessary antibiotic therapy in general NTS gastroenteritis are important. For severe or invasive infections caused by NTS infection in Southwest China, the use of ceftazidime is recommended until antibiotic sensitivity test results are available. And the choice of antibiotics should be based on the curative effect and the antibiotics sensitivity results.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prevalence, Clinical Characteristics and Changes of Antibiotic Resistance in Children with Nontyphoidal Salmonella Infections from 2009–2018 in Chongqing, China

Wu¹,

Luo²,

Shi³

et al. 2021

IDR

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“…Six pairs of antibiotic-resistant gene and eleven pairs of SPI1-6 virulence gene amplification primers were designed based on the gene sequences in GenBank and the literature (5,(11)(12)(13)(14)(15)(16)(17)(18). All PCR products were sequenced using primer walking, starting with 3CS and 5CS primers (Table 1).…”

Section: Determination Of Antibiotic Resistance Genes and Spi1-6 Genesmentioning

confidence: 99%

Serotypes, Antibiotic Resistance Genes, and Salmonella Pathogenicity Island Genes of Salmonella from Patients in a Hospital in Weifang, China

Liu

et al. 2022

Jundishapur J Microbiol

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Background: Salmonella is an important foodborne pathogen that causes diarrhea in humans worldwide. Objectives: This study aimed to determine the serotype distribution, antibiotic-resistant genes, and Salmonella pathogenicity island (SPI) genes of clinical isolates of Salmonella in Weifang. Methods: A total of 111 Salmonella strains were collected from Weifang People’s Hospital between 2018 and 2020 and subjected to serotyping using the Kauffmann-White antigen table. Meanwhile, the polymerase chain reaction detected eleven SPI1-6 genes and six antibiotic resistance genes. Results: Among the 111 Salmonella strains, 17 serotypes were identified, with S. Typhimurium, S. Typhi, and S. Enteritidis being the most prevalent. The hilA, ssaB, sseC, marT, siiE, pipB, sopB, and pagN SPI1-6 genes were all found during analysis. The InvA, misL, and siiD genes were detected at 98.2, 97.30, and 97.30% rates, respectively. Also, sul2 and blaTEM were the most prevalent antibiotic resistance genes in this investigation, accounting for 68.47 and 21.62% of the total, respectively. Conclusions: Salmonella isolated from the clinical samples was found to have a diversity of serotypes and possessed various SPI and antibiotic resistance genes.

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“…Salmonella is an important foodborne pathogen that causes acute diarrhea in humans worldwide 1 . In approximately 5%–10% of patients, the condition is further complicated by bacteremia, which can be life threatening.…”

Section: Introductionmentioning

confidence: 99%

Multilocus sequence typing analysis and second‐generation sequencing analysis of Salmonella Wandsworth

Zhang

Wang

et al. 2021

Clinical Laboratory Analysis

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Background Salmonella Wandsworth is a rare serotype of Salmonella. This study analyzed the genotyping, genome structure, and molecular biological functions of Salmonella Wandsworth based on the results of multilocus sequence typing and next‐generation sequencing genome assembly analysis. Methods Serological typing was performed using the slide‐agglutination method. The micro broth dilution method was used to test antibiotic susceptibility. Multilocus sequence typing (MLST) was used to perform the homology analysis, while the second‐generation sequencing genome analysis was used to analyze the whole genome of the bacteria. Results Salmonella Wandsworth is Group Q Salmonella. The MLST of this strain was ST1498. Salmonella Wandsworth was sensitive to antibiotics, such as ceftriaxone, imipenem, chloramphenicol, and colistin, but was resistant to ampicillin, cefalotin, gentamicin, and ciprofloxacin. The second‐generation sequencing results showed that the genome sequence length of the bacteria was 5109457bp. Annotated COG library analysis generated 3,746 corresponding genes. After the comparison with the KEGG library, 1,340 genes, which participate in 19 types of metabolic pathways, were obtained. A total of 249 pathogenic factors and 2 disease islands were predicted. 2 CRISPR sites and 8 Cas sites were predicted. It can be seen from the evolutionary tree that Salmonella Wandsworth MLST1498 and Paratyphi B str.SPB7 are gathered together. We identified one resistance gene, namely, aac(6’)‐Iaa accounting for aminoglycoside resistance. Conclusion Salmonella Wandsworth isolated in this study is Salmonella group Q. Consequently, it is necessary to strengthen the understanding of clinical infections of Salmonella Wandsworth and carry out continuous monitoring and research.

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Serotypes, antibiotic resistance, and virulence genes of Salmonella in children with diarrhea

Abstract: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

Cited by 29 publications

References 18 publications

Prevalence, Clinical Characteristics and Changes of Antibiotic Resistance in Children with Nontyphoidal Salmonella Infections from 2009–2018 in Chongqing, China

Prevalence, Clinical Characteristics and Changes of Antibiotic Resistance in Children with Nontyphoidal Salmonella Infections from 2009–2018 in Chongqing, China

Serotypes, Antibiotic Resistance Genes, and Salmonella Pathogenicity Island Genes of Salmonella from Patients in a Hospital in Weifang, China

Multilocus sequence typing analysis and second‐generation sequencing analysis of Salmonella Wandsworth

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