Serotypes and clones causing invasive pneumococcal disease before the use of new conjugate vaccines in Catalonia, Spain

Muñoz-Almagro, Carmen; Ciruela, Pilar; Esteva, Cristina; Marco, Francesc; Navarro, Marian; Bartolomé, Rosa; Sauca, Goretti; Gallés, C; Morta, M.; Ballester, Frédéric; Raga, Xavier; Selva, Laura

doi:10.1016/j.jinf.2011.06.002

Cited by 43 publications

(34 citation statements)

References 34 publications

(25 reference statements)

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“…Although these numbers represent the most rigorous estimate of child deaths caused by S. pneumoniae, they are probably underestimated [1]. Many of these deaths could be prevented with vaccination [4]. S. pneumoniae includes more than 90 serotypes.…”

Section: Introductionmentioning

confidence: 99%

Safety and immunogenicity of 13-valent pneumococcal conjugate vaccine in infants: A meta-analysis

Ruiz-Aragón

Peláez

Molina-Linde

et al. 2013

Vaccine

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Section: Introductionmentioning

confidence: 99%

Safety and immunogenicity of 13-valent pneumococcal conjugate vaccine in infants: A meta-analysis

Ruiz-Aragón

Peláez

Molina-Linde

et al. 2013

Vaccine

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“…18 Criteria for SNPs selection were: (1) proteins in TIR pathway (which are critical points in signaling cascade pathway); (2) high frequency in general population (>5%); (3) clinical relevance in infectious or inflammatory diseases described in the literature and (4) location of SNPs in the corresponding gene (SNPs in exons or in promoter or regulatory regions). Finally, selected SNPs were: (1) IRAK1: rs1059701, rs1059702, rs1059703 8,15; (2) IRAKM: rs1624395, rs1370128 8,16; (3) IRAK4: rs1141168, rs4251513, rs1461567 7-9,14,17 and (4) MyD88: rs7744, rs6853 10.…”

Section: Methodsmentioning

confidence: 99%

“…IPD rates are especially high in infants and children less than 2 years old and adults older than 65 years of age. 2 The imbalance between host factors and virulence of the pathogen is partly responsible for the development of IPD. The main virulence factor of pneumococcus is the polysaccharide capsule, with 94 serotypes, with differences in pathogenicity.…”

mentioning

confidence: 99%

Association of Polymorphisms in IRAK1, IRAK4 and MyD88, and Severe Invasive Pneumococcal Disease

Carrasco-Colom

Jordán

Alsina

et al. 2015

Pediatric Infectious Disease Journal

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Background: Severe invasive pneumococcal disease (SIPD) has high morbidity and mortality, conditioned by pneumococcus and host factors, such as Toll-like receptors and their Toll-IL1R common signaling pathway. The objectives of this study are (1) to correlate single nucleotide polymorphisms (SNPs) involved in some Toll-IL1R signaling pathway proteins (IRAK1, IRAK4, IRAKM and MyD88) with SIPD by comparing patients versus healthy controls. (2) To determine whether these SNPs influence SIPD outcome. Methods: Case-control prospective observational study: 60 pediatric patients with IPD and systemic inflammatory response syndrome, and 120 healthy volunteers. Well-known immunodeficiencies were excluded. Independent variables: SNPs genotypes and alleles. Other variables: demographic, previous infections, and clinical, analytical and microbiological evolution data. Results: We have detected significant disequilibrium of SNPs frequencies between SIPD patients and controls in rs1059701-CC (IRAK1; P = 0.0067), rs4251513-CC (IRAK4; P < 0.0001), rs1461567-T (IRAK4; P = 0.0158) and rs6853-AA (MyD88; P < 0.0001). SIPD patients showed significant association between: leukocytosis > 15,000/mmc and rs1059702-nonTT (IRAK1; P = 0.0460), pleuropneumonia and rs1624395-G (IRAKM; P = 0.0147), and rs1370128-C (IRAKM; P = 0.0055), sequelae, and rs4251513-nonGG (IRAK4; P = 0.0055), death and rs6853-nonAA (P = 0.0054) and rs6853-G (P = 0.0065; MyD88). Conclusions: This is the first study to show an association between SNPs in IRAK1, IRAK4 and MyD88, and the presence of SIPD. Our data showed that some SNPs may lead to a higher risk of developing SIPD while other are related with the outcome in SIPD patients. Following PIRO score (predisposition, insult, response, organ dysfunction), identifying SNPs predisposing to infectious diseases, such as SIPD might help stratify patients with severe infectious diseases and design specific treatments.

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“…The department performs molecular surveillance of pneumococci from patients attending 30 health centers throughout the Catalonia region. These 30 health centers captured 40.5% of all hospital admissions and, specifically, 63% of pediatric hospital admissions during 2009 (27).…”

Section: Methodsmentioning

confidence: 99%

Rapid and Easy Identification of Capsular Serotypes of Streptococcus pneumoniae by Use of Fragment Analysis by Automated Fluorescence-Based Capillary Electrophoresis

et al. 2012

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The purpose of this study was to develop a high-throughput method for the identification of pneumococcal capsular types. Multiplex PCR combined with fragment analysis and automated fluorescent capillary electrophoresis (FAF-mPCR) was utilized. Only 11 results were discordant with the Quellung reaction. However, latex reaction and Quellung results of the second reference laboratory agreed with FAF-mPCR for 9 of these 11 strains (82%). Therefore, we considered that only 2 of 394 strains (0.5%) were not properly characterized by the new assay. The automation of the process allowed the typing of 30 isolates in a few hours with a lower cost than that of the Quellung reaction. These results indicate that FAF-mPCR is a good method to determine the capsular serotype of Streptococcus pneumoniae.

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Serotypes and clones causing invasive pneumococcal disease before the use of new conjugate vaccines in Catalonia, Spain

Cited by 43 publications

References 34 publications

Safety and immunogenicity of 13-valent pneumococcal conjugate vaccine in infants: A meta-analysis

Safety and immunogenicity of 13-valent pneumococcal conjugate vaccine in infants: A meta-analysis

Association of Polymorphisms in IRAK1, IRAK4 and MyD88, and Severe Invasive Pneumococcal Disease

Rapid and Easy Identification of Capsular Serotypes of Streptococcus pneumoniae by Use of Fragment Analysis by Automated Fluorescence-Based Capillary Electrophoresis

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